Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR0436Btlr/Mmmh
Stock Number:
038637-MU
Citation ID:
RRID:MMRRC_038637-MU
Other Names:
R0436 (G1), C57BL/6J-MtgxR0436Btlr
Major Collection:

Strain Information

Zdhhc17
Name: zinc finger, DHHC domain containing 17
Synonyms: A230053P19Rik, D130071N24Rik, Hip14
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320150
VEGA: 10
Homologene: 56324
Nae1
Name: NEDD8 activating enzyme E1 subunit 1
Synonyms: Appbp1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234664
HGNC: HGNC:621
Homologene: 68370
Slc12a8
Name: solute carrier family 12 (potassium/chloride transporters), member 8
Synonyms: E330020C02Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 171286
Homologene: 11628
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Odf2l
Name: outer dense fiber of sperm tails 2-like
Synonyms: 4733401D09Rik, 9630045K08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 52184
Homologene: 12011
Gtf2a1
Name: general transcription factor II A, 1
Synonyms: TfIIAa/b, 19kDa, 37kDa, 6330549H03Rik, Tfiia1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 83602
HGNC: HGNC:4646
Homologene: 56331
Tnks2
Name: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
Synonyms: 5430432P15Rik, Parp5b
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 74493
Homologene: 11890
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 135,146,980 bp
  • T to C, chromosome 1 at 150,104,277 bp
  • A to C, chromosome 1 at 162,705,314 bp
  • A to T, chromosome 1 at 180,972,733 bp
  • A to G, chromosome 2 at 31,405,612 bp
  • A to G, chromosome 2 at 32,230,660 bp
  • A to T, chromosome 2 at 93,668,357 bp
  • T to C, chromosome 2 at 101,610,519 bp
  • G to C, chromosome 2 at 104,167,950 bp
  • A to T, chromosome 2 at 120,102,341 bp
  • A to G, chromosome 3 at 27,150,095 bp
  • A to G, chromosome 3 at 88,808,655 bp
  • T to A, chromosome 3 at 105,960,026 bp
  • C to T, chromosome 3 at 145,126,116 bp
  • T to C, chromosome 4 at 62,543,445 bp
  • T to C, chromosome 4 at 86,585,807 bp
  • T to C, chromosome 5 at 137,464,902 bp
  • T to A, chromosome 6 at 29,414,934 bp
  • A to G, chromosome 6 at 47,523,773 bp
  • T to A, chromosome 6 at 56,956,962 bp
  • A to G, chromosome 7 at 45,336,133 bp
  • G to A, chromosome 7 at 46,265,936 bp
  • A to G, chromosome 7 at 103,358,741 bp
  • G to A, chromosome 7 at 130,786,053 bp
  • T to C, chromosome 8 at 104,523,236 bp
  • C to T, chromosome 8 at 123,971,001 bp
  • C to T, chromosome 8 at 124,548,514 bp
  • C to T, chromosome 9 at 4,385,180 bp
  • T to C, chromosome 9 at 54,383,750 bp
  • T to C, chromosome 9 at 88,588,187 bp
  • C to T, chromosome 9 at 121,788,762 bp
  • A to T, chromosome 10 at 62,299,275 bp
  • G to T, chromosome 10 at 93,038,975 bp
  • A to T, chromosome 10 at 110,981,990 bp
  • C to T, chromosome 10 at 120,094,212 bp
  • T to C, chromosome 11 at 82,929,567 bp
  • C to T, chromosome 11 at 100,746,932 bp
  • A to G, chromosome 11 at 106,818,685 bp
  • T to C, chromosome 12 at 80,490,770 bp
  • A to C, chromosome 12 at 91,568,273 bp
  • A to G, chromosome 12 at 104,946,924 bp
  • C to T, chromosome 14 at 30,970,472 bp
  • T to C, chromosome 14 at 32,608,083 bp
  • C to A, chromosome 14 at 33,083,812 bp
  • T to A, chromosome 16 at 33,551,085 bp
  • T to A, chromosome 16 at 90,464,154 bp
  • A to G, chromosome 17 at 34,159,656 bp
  • A to G, chromosome 17 at 78,935,650 bp
  • C to T, chromosome 17 at 88,565,689 bp
  • A to G, chromosome 18 at 36,664,301 bp
  • G to A, chromosome 18 at 43,558,169 bp
  • A to G, chromosome 18 at 62,179,553 bp
  • A to G, chromosome 19 at 36,849,358 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0436 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038637-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.