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Strain Name:
C57BL/6J-MtgxR0442Btlr/Mmmh
Stock Number:
038643-MU
Citation ID:
RRID:MMRRC_038643-MU
Other Names:
R0442 (G1), C57BL/6J-MtgxR0442Btlr
Major Collection:

Strain Information

Pds5b
Name: PDS5 cohesin associated factor B
Synonyms: AS3, Aprin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100710
Homologene: 41001
Plekhm1
Name: pleckstrin homology domain containing, family M (with RUN domain) member 1
Synonyms: D330036J23Rik, AP162, B2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 353047
Homologene: 8871
Fdft1
Name: farnesyl diphosphate farnesyl transferase 1
Synonyms: squalene synthase, SQS, Ss
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 14137
VEGA: 14
HGNC: HGNC:3629
Homologene: 3281
Cep128
Name: centrosomal protein 128
Synonyms: 5430424K18Rik, 4930534B04Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 75216
Homologene: 35315
Iqgap3
Name: IQ motif containing GTPase activating protein 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 404710
Homologene: 26400
Snrnp40
Name: small nuclear ribonucleoprotein 40 (U5)
Synonyms: 0610009C03Rik, Wdr57
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66585
Homologene: 3538
Hjurp
Name: Holliday junction recognition protein
Synonyms: C330011F01Rik, A730008H23Rik, 6430706D22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381280
Homologene: 10184
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 4,346,747 bp
  • T to A, chromosome 1 at 25,396,470 bp
  • AT to A, chromosome 1 at 88,212,371 bp
  • GT to GTT, chromosome 1 at 88,266,524 bp
  • A to G, chromosome 1 at 161,032,348 bp
  • A to T, chromosome 2 at 25,587,522 bp
  • T to C, chromosome 2 at 70,238,961 bp
  • T to C, chromosome 2 at 122,289,332 bp
  • T to C, chromosome 2 at 153,870,952 bp
  • T to A, chromosome 2 at 181,232,209 bp
  • A to G, chromosome 3 at 15,802,546 bp
  • C to T, chromosome 3 at 88,115,959 bp
  • A to T, chromosome 3 at 89,151,540 bp
  • G to A, chromosome 4 at 11,609,480 bp
  • C to A, chromosome 4 at 11,610,362 bp
  • C to G, chromosome 4 at 130,378,043 bp
  • T to G, chromosome 5 at 37,135,553 bp
  • T to A, chromosome 5 at 121,323,982 bp
  • T to C, chromosome 5 at 150,716,544 bp
  • G to T, chromosome 6 at 48,678,066 bp
  • T to G, chromosome 6 at 125,339,137 bp
  • T to C, chromosome 6 at 130,372,872 bp
  • T to C, chromosome 7 at 6,394,999 bp
  • T to C, chromosome 7 at 119,843,285 bp
  • C to T, chromosome 8 at 21,963,058 bp
  • T to C, chromosome 8 at 71,367,058 bp
  • T to A, chromosome 8 at 71,491,491 bp
  • T to C, chromosome 10 at 18,677,815 bp
  • A to G, chromosome 10 at 21,126,196 bp
  • A to G, chromosome 10 at 23,920,482 bp
  • T to A, chromosome 10 at 107,876,855 bp
  • T to C, chromosome 10 at 129,659,824 bp
  • T to A, chromosome 11 at 58,831,431 bp
  • A to T, chromosome 11 at 59,002,174 bp
  • A to G, chromosome 11 at 59,874,619 bp
  • C to A, chromosome 11 at 69,448,542 bp
  • T to C, chromosome 11 at 74,084,495 bp
  • A to G, chromosome 11 at 103,397,174 bp
  • G to A, chromosome 12 at 24,612,170 bp
  • C to T, chromosome 12 at 91,266,771 bp
  • T to A, chromosome 13 at 100,444,516 bp
  • T to C, chromosome 14 at 20,607,209 bp
  • T to C, chromosome 14 at 55,066,900 bp
  • T to C, chromosome 14 at 63,163,349 bp
  • A to T, chromosome 15 at 78,540,397 bp
  • T to A, chromosome 16 at 45,397,155 bp
  • C to A, chromosome 16 at 58,473,453 bp
  • G to T, chromosome 16 at 58,473,455 bp
  • A to T, chromosome 18 at 5,046,870 bp
  • T to G, chromosome 19 at 13,480,048 bp
  • T to C, chromosome 19 at 32,493,948 bp
  • T to G, chromosome 19 at 44,566,012 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0442 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038643-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.