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Strain Name:
C57BL/6J-MtgxR0443Btlr/Mmmh
Stock Number:
038644-MU
Citation ID:
RRID:MMRRC_038644-MU
Other Names:
R0443 (G1), C57BL/6J-MtgxR0443Btlr
Major Collection:

Strain Information

Pknox1
Name: Pbx/knotted 1 homeobox
Synonyms: PREP1, D17Wsu76e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18771
HGNC: HGNC:9022
Homologene: 3363
Trpc2
Name: transient receptor potential cation channel, subfamily C, member 2
Synonyms: TRPC2b, TRPC2a, mTrp2, trp2, Trrp2, 3010009O07Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22064
Homologene: 135989
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Ttc17
Name: tetratricopeptide repeat domain 17
Synonyms: D2Bwg1005e, 9130020K17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74569
Homologene: 10100
Mtf1
Name: metal response element binding transcription factor 1
Synonyms: MTF-1, metal response element-binding transcription factor 1, metalloregulatory transcription factor, Thyls
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17764
HGNC: HGNC:7428
Homologene: 4347
Gapvd1
Name: GTPase activating protein and VPS9 domains 1
Synonyms: 2010005B09Rik, 4432404J10Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66691
Homologene: 32637
Golga1
Name: golgin A1
Synonyms: golgin-97, 0710001G09Rik, 2210418B03Rik, Golgi97, awag
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76899
HGNC: HGNC:4424
Homologene: 68223
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 19,104,367 bp
  • A to G, chromosome 1 at 34,294,550 bp
  • A to G, chromosome 1 at 65,196,706 bp
  • A to T, chromosome 1 at 127,822,415 bp
  • A to G, chromosome 1 at 143,765,923 bp
  • A to T, chromosome 1 at 156,681,453 bp
  • T to C, chromosome 2 at 30,403,628 bp
  • A to G, chromosome 2 at 34,704,621 bp
  • A to T, chromosome 2 at 39,018,441 bp
  • T to C, chromosome 2 at 52,161,477 bp
  • A to G, chromosome 2 at 94,378,094 bp
  • G to A, chromosome 2 at 156,016,787 bp
  • A to G, chromosome 2 at 163,858,323 bp
  • C to T, chromosome 4 at 124,824,282 bp
  • C to G, chromosome 4 at 130,378,043 bp
  • T to C, chromosome 4 at 148,948,911 bp
  • T to A, chromosome 4 at 155,269,140 bp
  • T to A, chromosome 5 at 8,447,348 bp
  • G to T, chromosome 5 at 25,848,411 bp
  • A to G, chromosome 5 at 33,388,824 bp
  • T to C, chromosome 5 at 38,200,830 bp
  • T to G, chromosome 5 at 96,091,745 bp
  • C to T, chromosome 5 at 121,887,437 bp
  • T to A, chromosome 6 at 41,771,895 bp
  • G to T, chromosome 6 at 132,951,196 bp
  • G to A, chromosome 7 at 10,748,311 bp
  • T to A, chromosome 7 at 17,095,163 bp
  • A to G, chromosome 7 at 102,093,520 bp
  • A to G, chromosome 7 at 108,124,816 bp
  • C to T, chromosome 7 at 128,081,634 bp
  • T to C, chromosome 8 at 24,629,637 bp
  • G to T, chromosome 9 at 7,167,244 bp
  • A to G, chromosome 9 at 50,621,103 bp
  • T to C, chromosome 10 at 24,595,803 bp
  • A to G, chromosome 11 at 73,425,755 bp
  • T to C, chromosome 11 at 75,544,667 bp
  • C to T, chromosome 11 at 98,657,688 bp
  • T to A, chromosome 13 at 22,328,071 bp
  • T to A, chromosome 13 at 114,992,460 bp
  • A to G, chromosome 14 at 55,753,748 bp
  • T to C, chromosome 15 at 76,639,684 bp
  • C to T, chromosome 17 at 24,505,400 bp
  • A to G, chromosome 17 at 31,592,219 bp
  • A to G, chromosome 18 at 36,644,599 bp
  • A to C, chromosome 18 at 37,412,432 bp
  • T to C, chromosome 18 at 77,955,903 bp
  • A to T, chromosome 19 at 5,272,583 bp
  • A to G, chromosome 19 at 11,521,680 bp
  • G to T, chromosome 19 at 45,008,139 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0443 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038644-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.