Strain Name:
C57BL/6J-MtgxR0452Btlr/Mmmh
Stock Number:
038652-MU
Citation ID:
RRID:MMRRC_038652-MU
Other Names:
R0452 (G1), C57BL/6J-MtgxR0452Btlr
Major Collection:

Gene Information

Gene Symbol: Cds2 [MGI:1332236] (Mus musculus (mouse))
Name: CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2
Synonyms: D2Wsu127e, 5730460C18Rik, 5730450N06Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Card11 [MGI:1916978] (Mus musculus (mouse))
Name: caspase recruitment domain family, member 11
Synonyms: BIMP3, 0610008L17Rik, 2410011D02Rik, CARMA1
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Jmjd1c [MGI:1918614] (Mus musculus (mouse))
Name: jumonji domain containing 1C
Synonyms: 5430433L24Rik, TRIP8, D630035I23Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Rnf145 [MGI:1921565] (Mus musculus (mouse))
Name: ring finger protein 145
Synonyms: 3732413I11Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Il17rd [MGI:2159727] (Mus musculus (mouse))
Name: interleukin 17 receptor D
Synonyms: Sef-S, Sef, 2810004A10Rik
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Plcd3 [MGI:107451] (Mus musculus (mouse))
Name: phospholipase C, delta 3
Synonyms: 2610205J15Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Ufc1 [MGI:1913405] (Mus musculus (mouse))
Name: ubiquitin-fold modifier conjugating enzyme 1
Synonyms: 1110021H02Rik, ESTM29
Chromosome: 1
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 16,602,255 bp
  • A to G, chromosome 1 at 34,437,621 bp
  • G to A, chromosome 1 at 34,732,322 bp
  • A to T, chromosome 1 at 53,605,819 bp
  • A to C, chromosome 1 at 164,185,107 bp
  • A to G, chromosome 1 at 170,212,402 bp
  • C to T, chromosome 1 at 171,289,954 bp
  • G to T, chromosome 2 at 28,695,993 bp
  • A to T, chromosome 2 at 32,281,824 bp
  • G to A, chromosome 2 at 58,500,495 bp
  • T to A, chromosome 2 at 76,836,003 bp
  • A to G, chromosome 2 at 76,871,110 bp
  • T to A, chromosome 2 at 112,092,636 bp
  • T to C, chromosome 2 at 112,466,969 bp
  • T to A, chromosome 2 at 113,636,779 bp
  • C to T, chromosome 2 at 132,298,479 bp
  • A to T, chromosome 3 at 69,008,028 bp
  • A to T, chromosome 4 at 45,965,488 bp
  • C to T, chromosome 4 at 133,689,105 bp
  • G to A, chromosome 4 at 138,310,188 bp
  • C to A, chromosome 4 at 155,902,328 bp
  • A to T, chromosome 5 at 65,264,297 bp
  • A to G, chromosome 5 at 98,997,520 bp
  • A to T, chromosome 5 at 107,140,423 bp
  • A to T, chromosome 5 at 120,694,992 bp
  • A to G, chromosome 5 at 140,880,370 bp
  • A to G, chromosome 6 at 30,487,381 bp
  • T to A, chromosome 6 at 38,307,437 bp
  • A to G, chromosome 6 at 40,759,090 bp
  • A to G, chromosome 6 at 41,445,337 bp
  • C to G, chromosome 6 at 53,604,542 bp
  • A to G, chromosome 6 at 57,926,484 bp
  • A to T, chromosome 6 at 65,074,822 bp
  • A to G, chromosome 6 at 85,478,182 bp
  • G to T, chromosome 6 at 86,104,629 bp
  • G to A, chromosome 6 at 122,323,036 bp
  • T to C, chromosome 6 at 136,909,489 bp
  • A to G, chromosome 7 at 6,963,182 bp
  • G to A, chromosome 7 at 10,378,292 bp
  • A to G, chromosome 7 at 17,815,323 bp
  • A to C, chromosome 7 at 26,766,762 bp
  • T to C, chromosome 7 at 43,794,251 bp
  • G to A, chromosome 7 at 102,236,462 bp
  • C to T, chromosome 7 at 108,612,370 bp
  • C to A, chromosome 7 at 143,592,625 bp
  • A to G, chromosome 8 at 41,325,905 bp
  • A to T, chromosome 8 at 45,355,649 bp
  • A to T, chromosome 8 at 72,461,522 bp
  • A to G, chromosome 8 at 106,507,190 bp
  • C to T, chromosome 8 at 109,732,395 bp
  • G to T, chromosome 8 at 111,521,972 bp
  • C to T, chromosome 9 at 77,960,911 bp
  • T to A, chromosome 9 at 106,197,302 bp
  • T to C, chromosome 9 at 108,486,354 bp
  • T to A, chromosome 9 at 110,553,100 bp
  • T to A, chromosome 9 at 119,939,154 bp
  • A to T, chromosome 10 at 5,405,435 bp
  • T to C, chromosome 10 at 67,255,482 bp
  • T to C, chromosome 10 at 111,975,598 bp
  • T to C, chromosome 10 at 118,868,763 bp
  • T to A, chromosome 11 at 44,561,760 bp
  • T to A, chromosome 11 at 72,977,232 bp
  • T to A, chromosome 11 at 74,930,213 bp
  • G to T, chromosome 11 at 78,227,003 bp
  • T to C, chromosome 11 at 80,106,421 bp
  • A to G, chromosome 11 at 83,213,128 bp
  • A to T, chromosome 11 at 100,798,330 bp
  • C to A, chromosome 11 at 100,863,135 bp
  • A to T, chromosome 11 at 102,465,953 bp
  • G to A, chromosome 11 at 103,071,259 bp
  • A to T, chromosome 11 at 118,393,464 bp
  • T to A, chromosome 12 at 31,708,794 bp
  • T to A, chromosome 13 at 30,886,327 bp
  • T to A, chromosome 14 at 27,091,931 bp
  • T to A, chromosome 14 at 31,177,464 bp
  • C to A, chromosome 14 at 31,748,738 bp
  • A to T, chromosome 14 at 34,094,770 bp
  • A to T, chromosome 14 at 52,214,587 bp
  • T to C, chromosome 14 at 54,467,579 bp
  • A to G, chromosome 14 at 56,648,843 bp
  • C to A, chromosome 15 at 67,109,655 bp
  • T to G, chromosome 15 at 76,268,293 bp
  • C to T, chromosome 16 at 30,517,463 bp
  • A to T, chromosome 16 at 44,431,945 bp
  • T to C, chromosome 17 at 12,924,352 bp
  • C to A, chromosome 17 at 31,849,081 bp
  • T to C, chromosome 17 at 32,395,817 bp
  • T to C, chromosome 17 at 34,928,095 bp
  • A to T, chromosome 18 at 43,963,318 bp
  • T to A, chromosome 19 at 40,845,064 bp
  • A to G, chromosome 19 at 61,226,895 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0452 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038652-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.