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Strain Name:
C57BL/6J-MtgxR0463Btlr/Mmmh
Stock Number:
038663-MU
Citation ID:
RRID:MMRRC_038663-MU
Other Names:
R0463 (G1), C57BL/6J-MtgxR0463Btlr
Major Collection:

Strain Information

Kif5a
Name: kinesin family member 5A
Synonyms: Kns, Kif5, D10Bwg0738e, Khc
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16572
VEGA: 10
HGNC: HGNC:6323
Homologene: 55861
Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Mcoln3
Name: mucolipin 3
Synonyms: varitint-waddler, Va, 6720490O21Rik, TRPML3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 171166
Homologene: 10118
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Nrcam
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041
Itk
Name: IL2 inducible T cell kinase
Synonyms: Emt, Tsk, Tcsk
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16428
HGNC: HGNC:6171
Homologene: 4051
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 45,819,602 bp
  • T to C, chromosome 1 at 87,419,077 bp
  • A to C, chromosome 1 at 96,867,920 bp
  • C to A, chromosome 1 at 135,452,207 bp
  • C to T, chromosome 2 at 66,675,740 bp
  • A to G, chromosome 2 at 67,514,918 bp
  • T to A, chromosome 2 at 85,438,286 bp
  • T to C, chromosome 2 at 85,689,839 bp
  • A to T, chromosome 2 at 112,661,701 bp
  • T to A, chromosome 2 at 120,023,151 bp
  • C to A, chromosome 2 at 128,877,511 bp
  • A to G, chromosome 2 at 152,324,513 bp
  • T to A, chromosome 2 at 163,730,351 bp
  • A to T, chromosome 3 at 67,180,861 bp
  • A to G, chromosome 3 at 90,180,211 bp
  • A to G, chromosome 3 at 95,121,304 bp
  • T to A, chromosome 3 at 107,105,160 bp
  • A to G, chromosome 3 at 115,928,703 bp
  • A to G, chromosome 3 at 129,706,233 bp
  • A to G, chromosome 3 at 129,737,549 bp
  • A to G, chromosome 3 at 133,486,666 bp
  • A to T, chromosome 3 at 141,857,430 bp
  • T to A, chromosome 3 at 146,140,576 bp
  • A to T, chromosome 3 at 154,328,171 bp
  • G to A, chromosome 4 at 98,674,308 bp
  • C to T, chromosome 4 at 109,890,941 bp
  • T to C, chromosome 4 at 127,122,772 bp
  • C to T, chromosome 4 at 141,043,656 bp
  • A to G, chromosome 5 at 109,737,060 bp
  • T to C, chromosome 5 at 135,076,541 bp
  • T to C, chromosome 5 at 145,007,994 bp
  • T to C, chromosome 6 at 28,724,956 bp
  • A to T, chromosome 6 at 106,731,890 bp
  • T to C, chromosome 6 at 128,780,403 bp
  • T to C, chromosome 7 at 4,776,024 bp
  • T to A, chromosome 7 at 15,757,646 bp
  • T to A, chromosome 7 at 24,055,501 bp
  • T to A, chromosome 7 at 62,378,030 bp
  • C to T, chromosome 7 at 64,220,254 bp
  • T to C, chromosome 7 at 97,729,021 bp
  • T to C, chromosome 7 at 100,419,638 bp
  • C to T, chromosome 7 at 132,610,677 bp
  • T to C, chromosome 7 at 133,974,416 bp
  • A to G, chromosome 7 at 142,512,335 bp
  • T to A, chromosome 8 at 3,632,559 bp
  • T to C, chromosome 8 at 13,409,279 bp
  • T to C, chromosome 8 at 15,104,123 bp
  • T to C, chromosome 8 at 15,921,759 bp
  • T to A, chromosome 8 at 33,280,815 bp
  • G to A, chromosome 8 at 110,506,627 bp
  • G to A, chromosome 9 at 38,209,064 bp
  • T to A, chromosome 9 at 103,426,655 bp
  • A to T, chromosome 9 at 106,958,506 bp
  • ACCC to ACC, chromosome 9 at 114,421,744 bp
  • CCCTCCTCCTCCTCCTCCTC to CCCTCCTCCTCCTCCTC, chromosome 9 at 119,972,775 bp
  • A to G, chromosome 10 at 33,466,421 bp
  • A to G, chromosome 10 at 40,040,616 bp
  • A to G, chromosome 10 at 54,074,498 bp
  • A to T, chromosome 10 at 63,052,471 bp
  • A to G, chromosome 10 at 92,874,943 bp
  • A to T, chromosome 10 at 127,235,652 bp
  • A to G, chromosome 10 at 129,681,839 bp
  • A to T, chromosome 11 at 46,331,989 bp
  • G to A, chromosome 11 at 50,145,067 bp
  • A to T, chromosome 11 at 55,262,829 bp
  • A to T, chromosome 11 at 59,061,530 bp
  • A to T, chromosome 11 at 69,423,126 bp
  • T to C, chromosome 11 at 72,351,952 bp
  • G to A, chromosome 11 at 87,893,196 bp
  • A to G, chromosome 11 at 100,349,305 bp
  • C to T, chromosome 11 at 101,331,606 bp
  • A to T, chromosome 11 at 102,864,771 bp
  • T to A, chromosome 12 at 44,551,341 bp
  • G to A, chromosome 12 at 76,418,967 bp
  • A to T, chromosome 12 at 102,562,951 bp
  • A to T, chromosome 13 at 8,203,188 bp
  • C to T, chromosome 13 at 63,520,307 bp
  • T to C, chromosome 13 at 64,778,876 bp
  • A to T, chromosome 14 at 20,448,563 bp
  • A to C, chromosome 14 at 21,423,536 bp
  • A to T, chromosome 14 at 70,522,670 bp
  • A to G, chromosome 14 at 70,532,860 bp
  • T to C, chromosome 15 at 35,597,409 bp
  • C to A, chromosome 15 at 36,092,938 bp
  • T to A, chromosome 15 at 66,380,474 bp
  • T to C, chromosome 15 at 83,001,483 bp
  • C to T, chromosome 15 at 91,159,124 bp
  • T to C, chromosome 17 at 32,975,962 bp
  • T to C, chromosome 17 at 35,822,108 bp
  • T to A, chromosome 17 at 43,625,561 bp
  • A to G, chromosome 17 at 56,659,554 bp
  • A to G, chromosome 17 at 67,896,225 bp
  • A to T, chromosome 18 at 24,254,525 bp
  • T to C, chromosome 18 at 46,178,456 bp
  • A to T, chromosome 18 at 82,609,282 bp
  • A to G, chromosome 19 at 9,009,407 bp
  • T to C, chromosome 19 at 37,916,504 bp
  • A to G, chromosome 19 at 42,749,416 bp
  • T to C, chromosome 19 at 44,550,345 bp
  • C to T, chromosome 19 at 47,143,841 bp
  • T to A, chromosome 19 at 58,738,196 bp
  • A to G, chromosome X at 106,578,655 bp
  • A to T, chromosome X at 133,962,187 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0463 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038663-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.