Strain Name:
C57BL/6J-MtgxR0549Btlr/Mmmh
Stock Number:
038741-MU
Citation ID:
RRID:MMRRC_038741-MU
Other Names:
R0549 (G1), C57BL/6J-MtgxR0549Btlr
Major Collection:

Gene Information

Sirt3
Name: sirtuin 3
Synonyms: Sir2l3, 2310003L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 64384
Homologene: 81827
Arhgap39
Name: Rho GTPase activating protein 39
Synonyms: D15Wsu169e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223666
Homologene: 27825
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Ncbp1
Name: nuclear cap binding protein subunit 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 433702
HGNC: HGNC:7658
Homologene: 1859
Smpd4
Name: sphingomyelin phosphodiesterase 4
Synonyms: neutral membrane (neutral sphingomyelinase-3), 4122402O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 77626
Homologene: 9813
Lrrc28
Name: leucine rich repeat containing 28
Synonyms: 2310058O11Rik, 2210012C09Rik, 1300004K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67867
Homologene: 16944
Tnfrsf21
Name: tumor necrosis factor receptor superfamily, member 21
Synonyms: DR6, Death receptor 6, TR7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 94185
VEGA: 17
Homologene: 8696
Prpf39
Name: pre-mRNA processing factor 39
Synonyms: Srcs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 328110
Homologene: 32377
Sidt2
Name: SID1 transmembrane family, member 2
Synonyms: CGI-40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214597
Homologene: 22943
Angptl3
Name: angiopoietin-like 3
Synonyms: hypl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 30924
HGNC: HGNC:491
Homologene: 8499
Cnpy4
Name: canopy FGF signaling regulator 4
Synonyms: 2610019P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66455
Homologene: 15196
Pcca
Name: propionyl-Coenzyme A carboxylase, alpha polypeptide
Synonyms: C79630
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110821
HGNC: HGNC:8653
Homologene: 236
Lmbrd1
Name: LMBR1 domain containing 1
Synonyms: 0910001K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 68421
Homologene: 10156
Osbpl7
Name: oxysterol binding protein-like 7
Synonyms: 4933437E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71240
Homologene: 79672
Svil
Name: supervillin
Synonyms: B430302E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225115
Homologene: 25090
Gcgr
Name: glucagon receptor
Synonyms: GR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14527
HGNC: HGNC:4192
Homologene: 131
Myh6
Name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Synonyms: alpha myosin, Myhc-a, alpha cardiac MHC, cardiomyopathy, hypertrophic 1, Myhca, A830009F23Rik, alpha-MHC, alphaMHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 17888
HGNC: HGNC:7576
Homologene: 124414
Tnik
Name: TRAF2 and NCK interacting kinase
Synonyms: 1500031A17Rik, 4831440I19Rik, C530008O15Rik, C630040K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 665113
Homologene: 77943
Adamts6
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 6
Synonyms: ADAM-TS6, A930019D11Rik, b2b2228Clo, b2b2187.1Clo, b2b2182Clo, b2b2029Clo, b2b1879.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 108154
VEGA: 13
HGNC: HGNC:222
Homologene: 82573
Ush2a
Name: usherin
Synonyms: MUSH2A, A930011D15Rik, LOC269160, LOC381317, A930037M10Rik, Ushrn, Ush2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22283
Homologene: 66151
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 672511
Homologene: 45439
Gm5316
Name: predicted gene 5316
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 102634348
Cdh7
Name: cadherin 7, type 2
Synonyms: CDH7L1, 9330156F07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241201
HGNC: HGNC:1766
Homologene: 68391
Tcp10a
Name: t-complex protein 10a
Synonyms: T66A-a, D17Leh66aa, D17Leh66A, Tcp-10a, Gm10326
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21461
Homologene: 83254
Mmp3
Name: matrix metallopeptidase 3
Synonyms: progelatinase, stromelysin 1, Str1, SLN1, SLN-1, stromelysin-1, Stmy1, STR-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17392
VEGA: 9
HGNC: HGNC:7173
Homologene: 20545
Vmn1r67
Name: vomeronasal 1 receptor 67
Synonyms: V1re10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 171263
Homologene: 128344
Dppa2
Name: developmental pluripotency associated 2
Synonyms: 2410088E07Rik, ECAT15-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 73703
VEGA: 16
Homologene: 79572
C4b
Name: complement component 4B (Chido blood group)
Synonyms: Ss, C4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12268
Homologene: 36030
Frmd4a
Name: FERM domain containing 4A
Synonyms: C230040M21Rik, 2700017I06Rik, Gm13190
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 209630
Homologene: 9971
Kif2b
Name: kinesin family member 2B
Synonyms: 1700063D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 73470
Homologene: 23775
Cfap65
Name: cilia and flagella associated protein 65
Synonyms: B230363K08Rik, Ccdc108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241116
Homologene: 28093
Tmem144
Name: transmembrane protein 144
Synonyms: 1110057I03Rik, 5730537D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 70652
Homologene: 41250
Col6a5
Name: collagen, type VI, alpha 5
Synonyms: Col6a5, Gm7455
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 665033
Homologene: 122792
Gria1
Name: glutamate receptor, ionotropic, AMPA1 (alpha 1)
Synonyms: GluR1, GluR-A, Glr-1, Glur-1, Glur1, GluRA, Glr1, 2900051M01Rik, HIPA1, GluA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14799
HGNC: HGNC:4571
Homologene: 20226
Pde1a
Name: phosphodiesterase 1A, calmodulin-dependent
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18573
HGNC: HGNC:8774
Homologene: 21043
Cfhr4
Name: complement factor H-related 4
Synonyms: Gm4788
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 214403
Evx2
Name: even-skipped homeobox 2
Synonyms: Evx-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14029
HGNC: HGNC:3507
Homologene: 74879
Agbl2
Name: ATP/GTP binding protein-like 2
Synonyms: A430081C19Rik, Ccp2, Ccp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 271813
Homologene: 11715
Hars2
Name: histidyl-tRNA synthetase 2
Synonyms: HARSR, HO3, 4631412B19Rik, Harsl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 70791
HGNC: HGNC:4817
Homologene: 40819
Vmn2r11
Name: vomeronasal 2, receptor 11
Synonyms: EG384219
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384219
Homologene: 129606
Nlrp5
Name: NLR family, pyrin domain containing 5
Synonyms: Op1, Mater, Nalp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23968
Homologene: 65105
Abcc10
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 10
Synonyms: Mrp7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224814
HGNC: HGNC:52
Homologene: 58616
Hkdc1
Name: hexokinase domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216019
Homologene: 128937
Sel1l2
Name: sel-1 suppressor of lin-12-like 2 (C. elegans)
Synonyms: LOC228684
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228684
Homologene: 16802
4930579C12Rik
Name: RIKEN cDNA 4930579C12 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319213
Pbxip1
Name: pre B cell leukemia transcription factor interacting protein 1
Synonyms: 4732463H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229534
Homologene: 10740
Papss1
Name: 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Synonyms: SK1, Asapk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 23971
HGNC: HGNC:8603
Homologene: 81740
Utp11
Name: UTP11 small subunit processome component
Synonyms: 2700082D03Rik, Utp11l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67205
Homologene: 6349
Olfr401
Name: olfactory receptor 401
Synonyms: GA_x6K02T2P1NL-4278037-4278984, MOR255-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258701
HGNC: HGNC:8282
Homologene: 73922
Ccl3
Name: chemokine (C-C motif) ligand 3
Synonyms: MIP1-(a), MIP1-alpha, MIP-1 alpha, MIP-1alpha, macrophage inflammatory protein-1alpha, Mip1a, CCL3, Scya3, LD78alpha, G0S19-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20302
Homologene: 2242
Nrsn1
Name: neurensin 1
Synonyms: Vmp, Neuro-p24, Neurensin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 22360
Homologene: 7597
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 24,744,920 bp
  • T to C, chromosome 1 at 74,918,444 bp
  • T to C, chromosome 1 at 110,108,944 bp
  • A to T, chromosome 1 at 139,739,488 bp
  • T to C, chromosome 1 at 188,946,953 bp
  • A to G, chromosome 2 at 4,603,967 bp
  • T to C, chromosome 2 at 74,659,134 bp
  • T to A, chromosome 2 at 79,865,070 bp
  • T to C, chromosome 2 at 90,789,843 bp
  • A to T, chromosome 2 at 140,265,882 bp
  • T to C, chromosome 3 at 28,570,920 bp
  • T to C, chromosome 3 at 79,822,744 bp
  • T to A, chromosome 3 at 89,443,592 bp
  • A to G, chromosome 3 at 131,619,213 bp
  • T to A, chromosome 4 at 46,168,476 bp
  • A to G, chromosome 4 at 99,031,455 bp
  • A to T, chromosome 4 at 124,686,079 bp
  • A to T, chromosome 5 at 109,052,097 bp
  • T to C, chromosome 5 at 138,187,637 bp
  • T to C, chromosome 6 at 122,900,191 bp
  • A to G, chromosome 7 at 10,447,714 bp
  • T to A, chromosome 7 at 23,441,802 bp
  • A to G, chromosome 7 at 67,628,342 bp
  • A to T, chromosome 7 at 140,869,487 bp
  • A to T, chromosome 9 at 7,455,638 bp
  • A to G, chromosome 9 at 45,953,119 bp
  • C to A, chromosome 9 at 89,168,912 bp
  • A to G, chromosome 9 at 105,904,579 bp
  • T to A, chromosome 10 at 62,400,240 bp
  • G to A, chromosome 11 at 57,228,973 bp
  • T to G, chromosome 11 at 74,121,475 bp
  • T to C, chromosome 11 at 79,468,771 bp
  • T to C, chromosome 11 at 83,648,336 bp
  • A to T, chromosome 11 at 91,576,584 bp
  • G to T, chromosome 11 at 97,067,542 bp
  • C to T, chromosome 11 at 119,465,082 bp
  • G to A, chromosome 11 at 120,536,561 bp
  • A to T, chromosome 12 at 65,056,256 bp
  • T to C, chromosome 13 at 25,262,258 bp
  • A to T, chromosome 13 at 104,297,255 bp
  • A to G, chromosome 14 at 54,958,608 bp
  • A to G, chromosome 14 at 122,638,377 bp
  • C to T, chromosome 15 at 76,734,886 bp
  • T to C, chromosome 16 at 17,639,312 bp
  • G to A, chromosome 16 at 48,318,671 bp
  • A to G, chromosome 17 at 7,326,551 bp
  • G to T, chromosome 17 at 34,735,415 bp
  • C to T, chromosome 17 at 43,038,213 bp
  • A to G, chromosome 17 at 46,322,290 bp
  • T to A, chromosome 18 at 5,064,566 bp
  • T to A, chromosome 18 at 36,786,208 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0549 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038741-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.