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Strain Name:
C57BL/6J-MtgxR0549Btlr/Mmmh
Stock Number:
038741-MU
Citation ID:
RRID:MMRRC_038741-MU
Other Names:
R0549 (G1), C57BL/6J-MtgxR0549Btlr
Major Collection:

Strain Information

Sirt3
Name: sirtuin 3
Synonyms: Sir2l3, 2310003L23Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 64384
Homologene: 81827
Arhgap39
Name: Rho GTPase activating protein 39
Synonyms: D15Wsu169e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223666
Homologene: 27825
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Ncbp1
Name: nuclear cap binding protein subunit 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 433702
HGNC: HGNC:7658
Homologene: 1859
Smpd4
Name: sphingomyelin phosphodiesterase 4
Synonyms: neutral membrane (neutral sphingomyelinase-3), 4122402O22Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 77626
Homologene: 9813
Lrrc28
Name: leucine rich repeat containing 28
Synonyms: 2310058O11Rik, 2210012C09Rik, 1300004K21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67867
Homologene: 16944
Tnfrsf21
Name: tumor necrosis factor receptor superfamily, member 21
Synonyms: DR6, Death receptor 6, TR7
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 94185
VEGA: 17
Homologene: 8696
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 24,744,920 bp
  • T to C, chromosome 1 at 74,918,444 bp
  • T to C, chromosome 1 at 110,108,944 bp
  • A to T, chromosome 1 at 139,739,488 bp
  • T to C, chromosome 1 at 188,946,953 bp
  • A to G, chromosome 2 at 4,603,967 bp
  • T to C, chromosome 2 at 74,659,134 bp
  • T to A, chromosome 2 at 79,865,070 bp
  • T to C, chromosome 2 at 90,789,843 bp
  • A to T, chromosome 2 at 140,265,882 bp
  • T to C, chromosome 3 at 28,570,920 bp
  • T to C, chromosome 3 at 79,822,744 bp
  • T to A, chromosome 3 at 89,443,592 bp
  • A to G, chromosome 3 at 131,619,213 bp
  • T to A, chromosome 4 at 46,168,476 bp
  • A to G, chromosome 4 at 99,031,455 bp
  • A to T, chromosome 4 at 124,686,079 bp
  • A to T, chromosome 5 at 109,052,097 bp
  • T to C, chromosome 5 at 138,187,637 bp
  • T to C, chromosome 6 at 122,900,191 bp
  • A to G, chromosome 7 at 10,447,714 bp
  • T to A, chromosome 7 at 23,441,802 bp
  • A to G, chromosome 7 at 67,628,342 bp
  • A to T, chromosome 7 at 140,869,487 bp
  • A to T, chromosome 9 at 7,455,638 bp
  • A to G, chromosome 9 at 45,953,119 bp
  • C to A, chromosome 9 at 89,168,912 bp
  • A to G, chromosome 9 at 105,904,579 bp
  • T to A, chromosome 10 at 62,400,240 bp
  • G to A, chromosome 11 at 57,228,973 bp
  • T to G, chromosome 11 at 74,121,475 bp
  • T to C, chromosome 11 at 79,468,771 bp
  • T to C, chromosome 11 at 83,648,336 bp
  • A to T, chromosome 11 at 91,576,584 bp
  • G to T, chromosome 11 at 97,067,542 bp
  • C to T, chromosome 11 at 119,465,082 bp
  • G to A, chromosome 11 at 120,536,561 bp
  • A to T, chromosome 12 at 65,056,256 bp
  • T to C, chromosome 13 at 25,262,258 bp
  • A to T, chromosome 13 at 104,297,255 bp
  • A to G, chromosome 14 at 54,958,608 bp
  • A to G, chromosome 14 at 122,638,377 bp
  • C to T, chromosome 15 at 76,734,886 bp
  • T to C, chromosome 16 at 17,639,312 bp
  • G to A, chromosome 16 at 48,318,671 bp
  • A to G, chromosome 17 at 7,326,551 bp
  • G to T, chromosome 17 at 34,735,415 bp
  • C to T, chromosome 17 at 43,038,213 bp
  • A to G, chromosome 17 at 46,322,290 bp
  • T to A, chromosome 18 at 5,064,566 bp
  • T to A, chromosome 18 at 36,786,208 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0549 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038741-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.