Strain Name:
C57BL/6J-MtgxR0588Btlr/Mmmh
Stock Number:
038778-MU
Citation ID:
RRID:MMRRC_038778-MU
Other Names:
R0588 (G1), C57BL/6J-MtgxR0588Btlr
Major Collection:

Gene Information

Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: Cav3.2, alpha13.2, T-type Cav3.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Map4k4
Name: mitogen-activated protein kinase kinase kinase kinase 4
Synonyms: Nik, 9430080K19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 26921
HGNC: HGNC:6866
Homologene: 7442
Cabin1
Name: calcineurin binding protein 1
Synonyms: Ppp3in, Cain, A330070M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 104248
VEGA: 10
Homologene: 49307
Fas
Name: Fas (TNF receptor superfamily member 6)
Synonyms: CD95, APO-1, TNFR6, Tnfrsf6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 14102
VEGA: 19
Homologene: 27
Ankrd13c
Name: ankyrin repeat domain 13c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 433667
Homologene: 41804
Gdap2
Name: ganglioside-induced differentiation-associated-protein 2
Synonyms: D3Ertd801e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14547
Homologene: 7728
Dcaf6
Name: DDB1 and CUL4 associated factor 6
Synonyms: PC326, 1200006M05Rik, Iqwd1, NRIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74106
Homologene: 10199
Crtc1
Name: CREB regulated transcription coactivator 1
Synonyms: Mect1, TORC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 382056
Homologene: 41607
Calcb
Name: calcitonin-related polypeptide, beta
Synonyms: CGRP2, Calc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 116903
HGNC: HGNC:1438
Homologene: 137656
Atp2a3
Name: ATPase, Ca++ transporting, ubiquitous
Synonyms: Serca3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 53313
HGNC: HGNC:813
Homologene: 69131
Gprc5b
Name: G protein-coupled receptor, family C, group 5, member B
Synonyms: hypothetical protein, clone 2-63
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 64297
Homologene: 9435
Fyb
Name: FYN binding protein
Synonyms: FYB-120/130, ADAP, B630013F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 23880
HGNC: HGNC:4036
Homologene: 22664
Adamts2
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 2
Synonyms: ADAM-TS2, hPCPNI, a disintegrin and metalloproteinase with thrombospondin repeats, procollagen N-proteinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216725
HGNC: HGNC:218
Homologene: 8597
Abcc9
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 9
Synonyms: SUR2B, SUR2A, Sur2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20928
HGNC: HGNC:60
Homologene: 56521
St18
Name: suppression of tumorigenicity 18
Synonyms: Nzf3, Myt3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240690
Homologene: 8792
Shisa9
Name: shisa family member 9
Synonyms: 2700045P11Rik, CKAMP44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 72555
Homologene: 109462
Arg1
Name: arginase, liver
Synonyms: PGIF, Arg-1, AI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11846
VEGA: 10
HGNC: HGNC:663
Homologene: 29
Olfr1449
Name: olfactory receptor 1449
Synonyms: GA_x6K02T2RE5P-3264213-3265157, MOR202-34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258300
Homologene: 17184
Zdhhc7
Name: zinc finger, DHHC domain containing 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102193
Homologene: 41193
Ears2
Name: glutamyl-tRNA synthetase 2, mitochondrial
Synonyms: 3230401I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67417
Homologene: 6907
Slc26a9
Name: solute carrier family 26, member 9
Synonyms: anion transporter/exchanger-9, E030002L01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 320718
Homologene: 14179
Lrrc69
Name: leucine rich repeat containing 69
Synonyms: 1700034K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 73314
Homologene: 78018
B230325K18Rik
Name: RIKEN cDNA B230325K18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Sostdc1
Name: sclerostin domain containing 1
Synonyms: Sostl, ectodin, USAG-1, Wise
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 66042
VEGA: 12
Homologene: 9154
Npy6r
Name: neuropeptide Y receptor Y6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 18169
VEGA: 18
HGNC: HGNC:7959
Homologene: 134692
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 6,817,738 bp
  • C to A, chromosome 1 at 40,004,864 bp
  • C to A, chromosome 1 at 131,754,011 bp
  • A to G, chromosome 1 at 165,420,223 bp
  • T to A, chromosome 3 at 100,170,001 bp
  • T to C, chromosome 3 at 158,005,817 bp
  • A to G, chromosome 4 at 14,704,001 bp
  • T to A, chromosome 6 at 142,603,061 bp
  • C to T, chromosome 7 at 114,720,126 bp
  • T to A, chromosome 7 at 118,983,995 bp
  • T to C, chromosome 7 at 122,044,291 bp
  • T to C, chromosome 7 at 127,985,574 bp
  • A to G, chromosome 8 at 70,439,549 bp
  • A to G, chromosome 8 at 120,083,367 bp
  • T to C, chromosome 10 at 24,920,624 bp
  • T to C, chromosome 10 at 75,745,337 bp
  • G to T, chromosome 11 at 50,776,664 bp
  • A to T, chromosome 11 at 72,973,024 bp
  • G to T, chromosome 12 at 36,317,021 bp
  • T to C, chromosome 15 at 6,580,459 bp
  • A to G, chromosome 16 at 12,267,774 bp
  • A to T, chromosome 17 at 25,387,564 bp
  • T to A, chromosome 18 at 44,275,821 bp
  • A to G, chromosome 19 at 12,934,747 bp
  • T to C, chromosome 19 at 34,327,140 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0588 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038778-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.