Strain Name:
C57BL/6J-MtgxR0606Btlr/Mmmh
Stock Number:
038795-MU
Citation ID:
RRID:MMRRC_038795-MU
Other Names:
R0606 (G1), C57BL/6J-MtgxR0606Btlr
Major Collection:

Gene Information

Gene Symbol: Atrn [MGI:1341628] (Mus musculus (mouse))
Name: attractin
Synonyms: Mgca
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Pdia3 [MGI:95834] (Mus musculus (mouse))
Name: protein disulfide isomerase associated 3
Synonyms: ERp60, Erp, ERp61, Plca, PDI-Q2, ERp57, Grp58, PDI
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Trnt1 [MGI:1917297] (Mus musculus (mouse))
Name: tRNA nucleotidyl transferase, CCA-adding, 1
Synonyms: CGI-47, 2610044E04Rik, 2410043H24Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Chst5 [MGI:1931825] (Mus musculus (mouse))
Name: carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5
Synonyms: I-GlcNAc6ST, GST-4
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Fryl [MGI:1919563] (Mus musculus (mouse))
Name: FRY like transcription coactivator
Synonyms: 2510002A14Rik, 2310004H21Rik, 9030227G01Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Lmna [MGI:96794] (Mus musculus (mouse))
Name: lamin A
Synonyms: Dhe, lamin A/C
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Cdk18 [MGI:97518] (Mus musculus (mouse))
Name: cyclin-dependent kinase 18
Synonyms: Pctk3
Chromosome: 1
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 38,048,893 bp
  • T to A, chromosome 1 at 38,059,123 bp
  • T to C, chromosome 1 at 82,672,586 bp
  • A to T, chromosome 1 at 83,280,424 bp
  • G to T, chromosome 1 at 132,117,617 bp
  • T to C, chromosome 1 at 135,959,901 bp
  • T to C, chromosome 1 at 139,423,610 bp
  • A to G, chromosome 1 at 149,840,704 bp
  • G to T, chromosome 2 at 30,104,797 bp
  • G to T, chromosome 2 at 88,185,280 bp
  • A to G, chromosome 2 at 91,682,073 bp
  • A to T, chromosome 2 at 120,773,872 bp
  • G to A, chromosome 2 at 121,432,377 bp
  • A to G, chromosome 2 at 130,906,856 bp
  • A to T, chromosome 2 at 150,570,519 bp
  • G to A, chromosome 3 at 86,906,004 bp
  • T to C, chromosome 3 at 88,482,578 bp
  • T to C, chromosome 4 at 33,132,696 bp
  • T to C, chromosome 4 at 43,547,756 bp
  • C to T, chromosome 4 at 44,042,244 bp
  • T to A, chromosome 4 at 143,949,883 bp
  • A to G, chromosome 5 at 29,590,928 bp
  • T to A, chromosome 5 at 31,088,723 bp
  • A to G, chromosome 5 at 31,254,313 bp
  • G to T, chromosome 5 at 72,580,543 bp
  • T to A, chromosome 5 at 73,124,734 bp
  • C to T, chromosome 5 at 76,312,481 bp
  • A to T, chromosome 5 at 102,897,220 bp
  • T to C, chromosome 5 at 109,943,481 bp
  • T to C, chromosome 5 at 118,259,089 bp
  • A to G, chromosome 5 at 149,588,130 bp
  • G to A, chromosome 6 at 24,501,261 bp
  • A to C, chromosome 6 at 29,753,604 bp
  • G to A, chromosome 6 at 37,871,234 bp
  • A to G, chromosome 6 at 54,629,820 bp
  • A to T, chromosome 6 at 91,026,929 bp
  • T to A, chromosome 6 at 106,777,908 bp
  • G to T, chromosome 6 at 119,926,683 bp
  • A to T, chromosome 6 at 131,220,224 bp
  • T to C, chromosome 7 at 35,394,963 bp
  • G to A, chromosome 7 at 97,347,601 bp
  • C to T, chromosome 8 at 110,549,798 bp
  • A to G, chromosome 8 at 111,890,919 bp
  • G to A, chromosome 8 at 122,892,832 bp
  • A to T, chromosome 8 at 128,722,372 bp
  • T to C, chromosome 9 at 53,556,481 bp
  • A to T, chromosome 9 at 64,179,300 bp
  • A to G, chromosome 9 at 73,530,983 bp
  • A to G, chromosome 9 at 121,749,290 bp
  • T to A, chromosome 9 at 124,028,802 bp
  • A to T, chromosome 10 at 36,832,588 bp
  • G to A, chromosome 10 at 127,444,444 bp
  • A to G, chromosome 11 at 4,782,194 bp
  • A to G, chromosome 11 at 50,874,185 bp
  • T to G, chromosome 11 at 60,830,746 bp
  • T to C, chromosome 11 at 65,841,333 bp
  • T to C, chromosome 11 at 75,514,348 bp
  • A to G, chromosome 11 at 83,863,984 bp
  • C to T, chromosome 11 at 85,345,877 bp
  • G to T, chromosome 11 at 96,034,973 bp
  • T to A, chromosome 11 at 100,702,251 bp
  • A to G, chromosome 11 at 118,263,028 bp
  • A to T, chromosome 12 at 80,174,647 bp
  • C to T, chromosome 12 at 98,943,459 bp
  • A to T, chromosome 13 at 27,314,194 bp
  • A to T, chromosome 13 at 38,016,494 bp
  • A to G, chromosome 13 at 41,767,405 bp
  • T to C, chromosome 13 at 59,702,431 bp
  • T to C, chromosome 13 at 112,504,272 bp
  • T to A, chromosome 14 at 12,154,131 bp
  • G to A, chromosome 14 at 20,391,389 bp
  • G to T, chromosome 14 at 51,906,217 bp
  • A to G, chromosome 14 at 57,638,208 bp
  • G to A, chromosome 14 at 69,191,006 bp
  • A to T, chromosome 14 at 77,029,621 bp
  • A to G, chromosome 15 at 34,345,150 bp
  • T to C, chromosome 15 at 48,457,662 bp
  • T to A, chromosome 15 at 58,899,827 bp
  • C to T, chromosome 16 at 18,935,047 bp
  • G to A, chromosome 16 at 35,856,760 bp
  • T to C, chromosome 16 at 37,204,521 bp
  • T to C, chromosome 17 at 19,678,844 bp
  • A to T, chromosome 17 at 32,527,843 bp
  • T to C, chromosome 17 at 33,433,598 bp
  • T to A, chromosome 17 at 33,658,390 bp
  • C to A, chromosome 17 at 84,416,303 bp
  • T to C, chromosome 17 at 90,565,373 bp
  • G to A, chromosome 18 at 53,917,028 bp
  • A to T, chromosome 19 at 4,361,964 bp
  • A to T, chromosome 19 at 11,752,294 bp
  • A to G, chromosome 19 at 58,999,940 bp
  • T to A, chromosome X at 64,151,653 bp
  • A to G, chromosome X at 93,488,087 bp
  • A to G, chromosome X at 136,453,511 bp
  • A to G, chromosome X at 141,192,223 bp
  • C to T, chromosome X at 150,398,364 bp
  • T to A, chromosome X at 152,347,040 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0606 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038795-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.