Strain Name:
C57BL/6J-MtgxR0633Btlr/Mmmh
Stock Number:
038822-MU
Citation ID:
RRID:MMRRC_038822-MU
Other Names:
R0633 (G1), C57BL/6J-MtgxR0633Btlr
Major Collection:

Gene Information

Gene Symbol: Mitf [MGI:104554] (Mus musculus (mouse))
Name: melanogenesis associated transcription factor
Synonyms: wh, Gsfbcc2, mi, bHLHe32, BCC2
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Cpe [MGI:101932] (Mus musculus (mouse))
Name: carboxypeptidase E
Synonyms: Cph1, Cph-1, NF-alpha1, CPH, carboxypeptidase H
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Lrp2 [MGI:95794] (Mus musculus (mouse))
Name: low density lipoprotein receptor-related protein 2
Synonyms: b2b1625.2Clo, Megalin, D230004K18Rik, Gp330
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Depdc7 [MGI:2139258] (Mus musculus (mouse))
Name: DEP domain containing 7
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Rgs3 [MGI:1354734] (Mus musculus (mouse))
Name: regulator of G-protein signaling 3
Synonyms: C2pa, PDZ-RGS3, C2PA-RGS3, RGS3S, 4930506N09Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Rngtt [MGI:1329041] (Mus musculus (mouse))
Name: RNA guanylyltransferase and 5'-phosphatase
Synonyms: mouse capping enzyme
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Ckap5 [MGI:1923036] (Mus musculus (mouse))
Name: cytoskeleton associated protein 5
Synonyms: 3110043H24Rik, D730027C18Rik, 4930432B04Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 130,188,526 bp
  • C to T, chromosome 1 at 136,527,305 bp
  • T to G, chromosome 1 at 153,844,107 bp
  • C to T, chromosome 2 at 20,855,387 bp
  • T to C, chromosome 2 at 52,112,563 bp
  • A to G, chromosome 2 at 66,236,233 bp
  • C to A, chromosome 2 at 69,448,120 bp
  • C to T, chromosome 2 at 76,724,195 bp
  • T to A, chromosome 2 at 86,162,091 bp
  • C to T, chromosome 2 at 86,445,129 bp
  • T to C, chromosome 2 at 89,609,374 bp
  • T to C, chromosome 2 at 91,550,743 bp
  • T to C, chromosome 2 at 104,722,881 bp
  • T to C, chromosome 2 at 121,308,014 bp
  • T to C, chromosome 2 at 127,539,525 bp
  • A to G, chromosome 2 at 160,903,974 bp
  • G to C, chromosome 2 at 181,570,490 bp
  • A to T, chromosome 3 at 82,045,460 bp
  • G to T, chromosome 3 at 100,684,575 bp
  • C to T, chromosome 3 at 151,801,763 bp
  • T to C, chromosome 4 at 33,368,690 bp
  • G to A, chromosome 4 at 62,625,906 bp
  • A to G, chromosome 4 at 140,757,585 bp
  • T to A, chromosome 4 at 154,142,491 bp
  • C to T, chromosome 4 at 155,858,295 bp
  • A to C, chromosome 5 at 29,451,100 bp
  • A to G, chromosome 5 at 52,554,460 bp
  • T to C, chromosome 5 at 106,917,601 bp
  • A to T, chromosome 5 at 122,800,632 bp
  • T to A, chromosome 6 at 18,305,980 bp
  • C to A, chromosome 6 at 98,003,904 bp
  • A to G, chromosome 6 at 106,679,248 bp
  • A to C, chromosome 6 at 108,144,671 bp
  • C to T, chromosome 6 at 116,420,384 bp
  • A to C, chromosome 6 at 125,081,991 bp
  • G to T, chromosome 6 at 146,374,456 bp
  • A to T, chromosome 6 at 149,325,701 bp
  • A to G, chromosome 7 at 66,400,222 bp
  • T to A, chromosome 7 at 78,843,935 bp
  • G to A, chromosome 7 at 79,411,157 bp
  • C to T, chromosome 7 at 105,558,963 bp
  • T to C, chromosome 7 at 106,713,977 bp
  • C to T, chromosome 7 at 118,490,256 bp
  • T to C, chromosome 8 at 11,550,511 bp
  • T to C, chromosome 8 at 22,580,388 bp
  • T to C, chromosome 8 at 39,620,000 bp
  • G to A, chromosome 8 at 64,609,203 bp
  • T to C, chromosome 8 at 70,294,684 bp
  • T to C, chromosome 9 at 8,112,068 bp
  • A to T, chromosome 9 at 21,844,417 bp
  • A to T, chromosome 9 at 30,943,511 bp
  • C to A, chromosome 9 at 35,545,972 bp
  • A to T, chromosome 9 at 48,396,597 bp
  • T to C, chromosome 9 at 80,394,155 bp
  • C to A, chromosome 9 at 120,388,236 bp
  • G to A, chromosome 10 at 10,391,729 bp
  • A to C, chromosome 10 at 23,806,038 bp
  • A to T, chromosome 10 at 26,302,685 bp
  • C to A, chromosome 10 at 80,307,455 bp
  • A to G, chromosome 10 at 85,907,903 bp
  • A to T, chromosome 10 at 115,220,310 bp
  • A to G, chromosome 11 at 11,769,223 bp
  • A to G, chromosome 11 at 59,428,613 bp
  • A to G, chromosome 11 at 73,516,927 bp
  • T to C, chromosome 12 at 111,345,555 bp
  • T to C, chromosome 13 at 49,558,907 bp
  • A to T, chromosome 13 at 67,754,844 bp
  • G to A, chromosome 14 at 14,857,782 bp
  • C to T, chromosome 14 at 26,927,890 bp
  • T to G, chromosome 14 at 95,881,943 bp
  • T to C, chromosome 14 at 110,751,885 bp
  • C to A, chromosome 15 at 68,180,803 bp
  • T to C, chromosome 16 at 57,049,414 bp
  • T to C, chromosome 16 at 58,731,290 bp
  • T to G, chromosome 17 at 29,040,760 bp
  • G to A, chromosome 17 at 35,901,423 bp
  • T to C, chromosome 17 at 74,729,977 bp
  • A to G, chromosome 17 at 87,672,810 bp
  • A to G, chromosome 17 at 90,704,181 bp
  • A to G, chromosome 18 at 34,586,266 bp
  • T to C, chromosome 18 at 63,865,300 bp
  • C to T, chromosome 18 at 86,404,729 bp
  • A to G, chromosome 19 at 4,941,782 bp
  • T to G, chromosome 19 at 7,457,593 bp
  • A to G, chromosome 19 at 7,788,210 bp
  • A to G, chromosome 19 at 10,531,782 bp
  • T to C, chromosome 19 at 13,633,336 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0633 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038822-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.