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Strain Name:
C57BL/6J-MtgxR0648Btlr/Mmmh
Stock Number:
038833-MU
Citation ID:
RRID:MMRRC_038833-MU
Other Names:
R0648 (G1), C57BL/6J-MtgxR0648Btlr
Major Collection:

Strain Information

Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Trpc2
Name: transient receptor potential cation channel, subfamily C, member 2
Synonyms: TRPC2b, TRPC2a, mTrp2, trp2, Trrp2, 3010009O07Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22064
Homologene: 135989
Xpnpep1
Name: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Synonyms: D230045I08Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 170750
Homologene: 6424
Yes1
Name: YES proto-oncogene 1, Src family tyrosine kinase
Synonyms: Yes
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22612
Homologene: 55900
Zfp42
Name: zinc finger protein 42
Synonyms: Rex-1, Zfp-42, Rex1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22702
Homologene: 7601
Polr1f
Name: RNA polymerase I subunit F
Synonyms: D16Wsu83e, 2810024J17Rik, Twistnb
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 28071
VEGA: 12
Homologene: 45411
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 44,056,563 bp
  • A to T, chromosome 1 at 71,597,585 bp
  • T to C, chromosome 1 at 75,427,978 bp
  • T to A, chromosome 1 at 110,065,607 bp
  • A to T, chromosome 1 at 136,304,319 bp
  • T to C, chromosome 1 at 143,695,462 bp
  • A to G, chromosome 1 at 152,536,265 bp
  • C to A, chromosome 1 at 172,067,887 bp
  • C to T, chromosome 1 at 180,600,440 bp
  • A to G, chromosome 1 at 183,281,209 bp
  • A to G, chromosome 2 at 121,235,707 bp
  • T to C, chromosome 2 at 180,943,112 bp
  • A to G, chromosome 3 at 58,676,214 bp
  • A to C, chromosome 3 at 85,730,614 bp
  • A to G, chromosome 3 at 89,216,665 bp
  • A to G, chromosome 3 at 96,221,535 bp
  • A to G, chromosome 3 at 106,500,555 bp
  • G to A, chromosome 3 at 135,230,082 bp
  • A to G, chromosome 4 at 56,906,270 bp
  • C to T, chromosome 4 at 118,690,072 bp
  • T to C, chromosome 4 at 129,578,357 bp
  • A to G, chromosome 4 at 133,685,204 bp
  • T to C, chromosome 5 at 7,305,215 bp
  • T to A, chromosome 5 at 32,655,518 bp
  • G to A, chromosome 5 at 63,924,745 bp
  • T to A, chromosome 5 at 108,135,560 bp
  • A to C, chromosome 5 at 108,174,987 bp
  • T to C, chromosome 5 at 108,995,916 bp
  • C to T, chromosome 5 at 120,959,270 bp
  • C to T, chromosome 5 at 122,251,698 bp
  • A to C, chromosome 6 at 51,879,785 bp
  • A to G, chromosome 6 at 57,360,475 bp
  • T to C, chromosome 6 at 115,994,001 bp
  • A to G, chromosome 6 at 129,330,969 bp
  • C to A, chromosome 6 at 147,055,945 bp
  • T to G, chromosome 7 at 45,519,786 bp
  • T to C, chromosome 7 at 102,078,824 bp
  • G to A, chromosome 8 at 11,246,892 bp
  • A to G, chromosome 8 at 43,295,978 bp
  • C to A, chromosome 8 at 71,256,530 bp
  • C to A, chromosome 8 at 85,073,282 bp
  • A to G, chromosome 8 at 105,844,960 bp
  • G to T, chromosome 8 at 122,454,994 bp
  • T to C, chromosome 9 at 50,898,745 bp
  • T to A, chromosome 9 at 108,544,877 bp
  • T to C, chromosome 9 at 108,563,466 bp
  • A to G, chromosome 10 at 18,531,726 bp
  • T to C, chromosome 10 at 26,989,376 bp
  • A to G, chromosome 10 at 130,017,481 bp
  • A to T, chromosome 11 at 44,991,510 bp
  • A to G, chromosome 11 at 78,550,539 bp
  • C to T, chromosome 12 at 33,438,000 bp
  • A to G, chromosome 12 at 76,451,296 bp
  • G to T, chromosome 12 at 81,314,446 bp
  • T to C, chromosome 12 at 102,742,517 bp
  • A to G, chromosome 12 at 104,314,679 bp
  • A to T, chromosome 13 at 11,724,333 bp
  • A to T, chromosome 13 at 21,318,877 bp
  • A to T, chromosome 13 at 26,769,853 bp
  • T to C, chromosome 13 at 49,296,573 bp
  • A to G, chromosome 13 at 63,844,645 bp
  • T to A, chromosome 13 at 69,627,587 bp
  • C to T, chromosome 14 at 87,472,182 bp
  • T to C, chromosome 15 at 7,207,709 bp
  • C to T, chromosome 15 at 55,603,201 bp
  • A to G, chromosome 15 at 81,016,920 bp
  • A to G, chromosome 15 at 83,933,064 bp
  • T to C, chromosome 15 at 96,418,458 bp
  • A to G, chromosome 16 at 20,365,882 bp
  • T to A, chromosome 16 at 43,662,038 bp
  • C to A, chromosome 17 at 5,493,602 bp
  • G to A, chromosome 17 at 24,594,937 bp
  • A to G, chromosome 17 at 35,982,598 bp
  • T to C, chromosome 17 at 71,229,312 bp
  • A to T, chromosome 18 at 43,999,797 bp
  • G to A, chromosome 19 at 8,874,248 bp
  • TGAAG to TGAAGAAG, chromosome 19 at 10,027,388 bp
  • T to C, chromosome 19 at 34,248,534 bp
  • T to A, chromosome 19 at 36,862,074 bp
  • A to G, chromosome 19 at 52,997,863 bp
  • C to T, chromosome X at 12,059,051 bp
  • A to T, chromosome X at 20,162,420 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0648 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038833-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.