Strain Name:
C57BL/6J-MtgxR0703Btlr/Mmmh
Stock Number:
038886-MU
Citation ID:
RRID:MMRRC_038886-MU
Other Names:
R0703 (G1), C57BL/6J-MtgxR0703Btlr
Major Collection:

Gene Information

Utp23
Name: UTP23 small subunit processome component
Synonyms: 1700010I21Rik, D530033C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 78581
Homologene: 41789
Polr3a
Name: polymerase (RNA) III (DNA directed) polypeptide A
Synonyms: RPC155, RPC1, 9330175N20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218832
VEGA: 14
Homologene: 5124
Hpgd
Name: hydroxyprostaglandin dehydrogenase 15 (NAD)
Synonyms: 15-PGDH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 15446
HGNC: HGNC:5154
Homologene: 68095
Adamts6
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 6
Synonyms: ADAM-TS6, A930019D11Rik, b2b1879.1Clo, b2b2228Clo, b2b2187.1Clo, b2b2182Clo, b2b2029Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 108154
VEGA: 13
HGNC: HGNC:222
Homologene: 82573
Cfap70
Name: cilia and flagella associated protein 70
Synonyms: 5330402L21Rik, Ttc18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 76670
Homologene: 17048
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, D830007G01Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Serpinb9b
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9b
Synonyms: 1600019A21Rik, ovalbumin, Spi10, R86, SPI-CI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20706
HGNC: HGNC:8955
Homologene: 137240
Socs4
Name: suppressor of cytokine signaling 4
Synonyms: 3110032M18Rik, Socs7, A730004F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67296
Homologene: 15442
Ldhb
Name: lactate dehydrogenase B
Synonyms: lactate dehydrogenase-B, Ldh-2, H-Ldh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16832
HGNC: HGNC:6541
Homologene: 55647
Lair1
Name: leukocyte-associated Ig-like receptor 1
Synonyms: 5133400O11Rik, mLair-1, Lair-1, D7Bwg0421e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 52855
Homologene: 48097
Gpam
Name: glycerol-3-phosphate acyltransferase, mitochondrial
Synonyms: GPAT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 14732
Homologene: 7343
Map4k2
Name: mitogen-activated protein kinase kinase kinase kinase 2
Synonyms: BL44, Rab8ip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 26412
HGNC: HGNC:6864
Homologene: 3370
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 2 at 76,939,841 bp
  • C to T, chromosome 6 at 142,495,601 bp
  • A to T, chromosome 7 at 4,010,760 bp
  • T to C, chromosome 8 at 56,295,039 bp
  • C to A, chromosome 13 at 33,032,981 bp
  • C to T, chromosome 13 at 104,352,847 bp
  • T to A, chromosome 14 at 20,439,715 bp
  • G to A, chromosome 14 at 24,484,164 bp
  • C to T, chromosome 14 at 47,290,048 bp
  • TGAGGAGGAGGAGGAGGA to TGAGGAGGAGGAGGA, chromosome 15 at 51,882,356 bp
  • T to C, chromosome 19 at 6,341,841 bp
  • A to G, chromosome 19 at 55,072,756 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0703 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038886-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.