Strain Name:
C57BL/6J-MtgxR0705Btlr/Mmmh
Stock Number:
038888-MU
Citation ID:
RRID:MMRRC_038888-MU
Other Names:
R0705 (G1), C57BL/6J-MtgxR0705Btlr
Major Collection:

Gene Information

Ecpas
Name: Ecm29 proteasome adaptor and scaffold
Synonyms: AI314180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230249
Homologene: 6056
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: Dnahc7, LOC381341, Dnahc7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 627872
Homologene: 41287
Crebbp
Name: CREB binding protein
Synonyms: KAT3A, CBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12914
HGNC: HGNC:2348
Homologene: 68393
Hpgd
Name: hydroxyprostaglandin dehydrogenase 15 (NAD)
Synonyms: 15-PGDH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 15446
HGNC: HGNC:5154
Homologene: 68095
Asic4
Name: acid-sensing (proton-gated) ion channel family member 4
Synonyms: Accn4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241118
Homologene: 11166
Spink5
Name: serine peptidase inhibitor, Kazal type 5
Synonyms: LEKT1, 2310065D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 72432
Homologene: 4987
Impg1
Name: interphotoreceptor matrix proteoglycan 1
Synonyms: IMP150, A930015H12Rik, SPACR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 63859
HGNC: HGNC:6055
Homologene: 1201
Fam234b
Name: family with sequence similarity 234, member B
Synonyms: 8430419L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74525
Homologene: 12572
Ccdc106
Name: coiled-coil domain containing 106
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232821
Homologene: 81845
Dpy19l3
Name: dpy-19-like 3 (C. elegans)
Synonyms: 9330164H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233115
Homologene: 18692
Irf9
Name: interferon regulatory factor 9
Synonyms: Isgf3g, p48, Irf-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16391
HGNC: HGNC:6131
Homologene: 4436
Fbxw22
Name: F-box and WD-40 domain protein 22
Synonyms: Gm5164
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382156
Homologene: 110776
Ssxb9
Name: synovial sarcoma, X member B9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 387131
Homologene: 85956
Kcnq5
Name: potassium voltage-gated channel, subfamily Q, member 5
Synonyms: 9230107O05Rik, D1Mgi1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226922
HGNC: HGNC:6299
Homologene: 28270
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 21,535,177 bp
  • T to A, chromosome 1 at 53,472,791 bp
  • G to T, chromosome 1 at 75,451,370 bp
  • A to T, chromosome 4 at 58,885,366 bp
  • A to T, chromosome 6 at 135,227,215 bp
  • G to A, chromosome 7 at 5,059,540 bp
  • T to A, chromosome 7 at 35,695,316 bp
  • T to C, chromosome 8 at 56,295,039 bp
  • A to T, chromosome 9 at 80,379,979 bp
  • G to T, chromosome 9 at 109,403,096 bp
  • T to A, chromosome 14 at 55,608,679 bp
  • A to C, chromosome 16 at 4,155,010 bp
  • T to A, chromosome 18 at 43,992,274 bp
  • T to G, chromosome X at 8,371,783 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0705 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038888-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.