Strain Name:
C57BL/6J-MtgxR0708Btlr/Mmmh
Stock Number:
038891-MU
Citation ID:
RRID:MMRRC_038891-MU
Other Names:
R0708 (G1), C57BL/6J-MtgxR0708Btlr
Major Collection:

Gene Information

Sptbn1
Name: spectrin beta, non-erythrocytic 1
Synonyms: beta fodrin, Spnb-2, spectrin G, brain spectrin, elf3, elf1, 9930031C03Rik, non-erythrocytic, Spnb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20742
Homologene: 2354
Hgf
Name: hepatocyte growth factor
Synonyms: scatter factor, NK1, SF/HGF, HGF/SF, NK2, C230052L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 15234
HGNC: HGNC:4893
Homologene: 503
Thbs4
Name: thrombospondin 4
Synonyms: TSP-4, TSP4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 21828
Homologene: 20691
Enox1
Name: ecto-NOX disulfide-thiol exchanger 1
Synonyms: D230005D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239188
VEGA: 14
Homologene: 56793
Ints14
Name: integrator complex subunit 14
Synonyms: 2010321M09Rik, Vwa9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69882
VEGA: 9
Homologene: 12774
Rab3gap2
Name: RAB3 GTPase activating protein subunit 2
Synonyms: 1110059F07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98732
Homologene: 40842
Orc3
Name: origin recognition complex, subunit 3
Synonyms: Orc3l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 50793
HGNC: HGNC:8489
Homologene: 8225
Frs2
Name: fibroblast growth factor receptor substrate 2
Synonyms: SNT1, C330018A15Rik, Frs2alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 327826
VEGA: 10
Homologene: 4846
Sema4d
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Synonyms: semaphorin H, M-sema G, coll-4, CD100, Semcl2, Semaj, Semacl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20354
Homologene: 21282
Sgcb
Name: sarcoglycan, beta (dystrophin-associated glycoprotein)
Synonyms: beta-SG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 24051
Homologene: 195
Tecr
Name: trans-2,3-enoyl-CoA reductase
Synonyms: 2410016D23Rik, SC2, A230102P12Rik, D17Ertd178e, Gpsn2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 106529
HGNC: HGNC:4551
Homologene: 36231
Nmbr
Name: neuromedin B receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18101
HGNC: HGNC:7843
Homologene: 20560
Cand2
Name: cullin-associated and neddylation-dissociated 2 (putative)
Synonyms: Tp120b, 2210404G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67088
Homologene: 41657
Hectd4
Name: HECT domain E3 ubiquitin protein ligase 4
Synonyms: Gm15800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269700
Homologene: 28297
Map4k1
Name: mitogen-activated protein kinase kinase kinase kinase 1
Synonyms: Hpk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26411
HGNC: HGNC:6863
Homologene: 5199
Insc
Name: INSC spindle orientation adaptor protein
Synonyms: Inscuteable, 3830422K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233752
Homologene: 78662
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: A730004I20Rik, Dnahc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Ptpro
Name: protein tyrosine phosphatase, receptor type, O
Synonyms: D28, PTPROt, PTP-phi, PTP-BK, PTP-U2, GLEPP1, PTP-oc, Ptpn15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 19277
HGNC: HGNC:9678
Homologene: 21564
Col9a1
Name: collagen, type IX, alpha 1
Synonyms: Col9a-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12839
HGNC: HGNC:2217
Homologene: 1393
Papss2
Name: 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Synonyms: Sk2, Atpsk2, 1810018P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 23972
VEGA: 19
HGNC: HGNC:8604
Homologene: 55840
Slc24a1
Name: solute carrier family 24 (sodium/potassium/calcium exchanger), member 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214111
VEGA: 9
Homologene: 3472
Prl8a8
Name: prolactin family 8, subfamily a, member 81
Synonyms: PLP-Cgamma, 1600032B14Rik, Prlpc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 74188
Homologene: 137338
Klk1b11
Name: kallikrein 1-related peptidase b11
Synonyms: mGK-11, Klk11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16613
HGNC: HGNC:6364
Homologene: 68141
Tectb
Name: tectorin beta
Synonyms: [b]-tectorin, Tctnb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 21684
Homologene: 7568
Tgs1
Name: trimethylguanosine synthase 1
Synonyms: Pimt, D4Ertd800e, Ncoa6ip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 116940
Homologene: 32608
Zfp558
Name: zinc finger protein 558
Synonyms: 1700007A21Rik, 4932704I17Rik, Zfp558-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72230
Homologene: 44903
Olfr926
Name: olfactory receptor 926
Synonyms: GA_x6K02T2PVTD-32573036-32573962, MOR171-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258811
VEGA: 9
HGNC: HGNC:8482
Homologene: 128215
Ogfod1
Name: 2-oxoglutarate and iron-dependent oxygenase domain containing 1
Synonyms: 4930415J21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270086
Homologene: 41238
Ptpn7
Name: protein tyrosine phosphatase, non-receptor type 7
Synonyms: LC-PTP, BPTP-4, C920001D21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 320139
HGNC: HGNC:9659
Homologene: 15411
Glra3
Name: glycine receptor, alpha 3 subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 110304
HGNC: HGNC:4328
Homologene: 142
Brdt
Name: bromodomain, testis-specific
Synonyms: 7420412D09Rik, Brd6, Fsrg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 114642
HGNC: HGNC:1105
Homologene: 21064
Gmppa
Name: GDP-mannose pyrophosphorylase A
Synonyms: 1810012N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 69080
Homologene: 8346
Tmem159
Name: transmembrane protein 159
Synonyms: 8430420C20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233806
Homologene: 10705
Poc1b
Name: POC1 centriolar protein B
Synonyms: 4933430F16Rik, Wdr51b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 382406
Homologene: 41728
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 24,237,261 bp
  • T to C, chromosome 1 at 75,442,574 bp
  • C to A, chromosome 1 at 135,134,547 bp
  • T to A, chromosome 1 at 185,249,926 bp
  • T to A, chromosome 4 at 3,586,152 bp
  • A to T, chromosome 4 at 34,597,368 bp
  • C to A, chromosome 5 at 16,566,763 bp
  • A to T, chromosome 5 at 73,640,882 bp
  • A to T, chromosome 5 at 107,358,900 bp
  • G to A, chromosome 5 at 121,286,463 bp
  • A to T, chromosome 6 at 73,212,622 bp
  • G to A, chromosome 6 at 115,803,805 bp
  • T to C, chromosome 6 at 137,386,253 bp
  • C to T, chromosome 7 at 28,998,449 bp
  • T to C, chromosome 7 at 43,997,728 bp
  • T to A, chromosome 7 at 114,845,146 bp
  • T to C, chromosome 7 at 120,116,214 bp
  • C to T, chromosome 8 at 56,125,364 bp
  • A to T, chromosome 8 at 83,573,109 bp
  • C to T, chromosome 8 at 94,039,045 bp
  • T to C, chromosome 9 at 18,456,827 bp
  • T to A, chromosome 9 at 38,877,275 bp
  • T to A, chromosome 9 at 64,947,890 bp
  • G to A, chromosome 9 at 64,983,984 bp
  • AAAT to AAATGAAT, chromosome 10 at 14,760,721 bp
  • A to G, chromosome 10 at 99,155,130 bp
  • G to A, chromosome 10 at 117,074,092 bp
  • A to T, chromosome 11 at 30,114,739 bp
  • A to T, chromosome 13 at 27,511,545 bp
  • A to G, chromosome 13 at 51,712,719 bp
  • C to A, chromosome 13 at 92,773,186 bp
  • A to G, chromosome 14 at 77,592,912 bp
  • T to C, chromosome 19 at 32,637,216 bp
  • T to C, chromosome 19 at 55,191,552 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0708 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038891-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.