Strain Name:
C57BL/6J-MtgxR0729Btlr/Mmmh
Stock Number:
038910-MU
Citation ID:
RRID:MMRRC_038910-MU
Other Names:
R0729 (G1), C57BL/6J-MtgxR0729Btlr
Major Collection:

Strain Information

Myo10
Name: myosin X
Synonyms: D15Ertd600e, myosin-X
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328
Sptbn1
Name: spectrin beta, non-erythrocytic 1
Synonyms: Spnb2, brain spectrin, spectrin G, elf3, elf1, 9930031C03Rik, Spnb-2, beta fodrin, non-erythrocytic
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20742
Homologene: 2354
Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 22427
Homologene: 6659
Htr2a
Name: 5-hydroxytryptamine (serotonin) receptor 2A
Synonyms: Htr2, Htr-2, 5-HT2A receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 15558
VEGA: 14
HGNC: HGNC:5293
Homologene: 68073
Sez6
Name: seizure related gene 6
Synonyms: D11Bhm177e, sez-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20370
Homologene: 10948
Capzb
Name: capping actin protein of muscle Z-line subunit beta
Synonyms: CPB1, CPbeta2, Cappb1, 1700120C01Rik, CPbeta1, CPB2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12345
HGNC: HGNC:1491
Homologene: 3620
Tmem63b
Name: transmembrane protein 63b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224807
Homologene: 101682
Ncoa7
Name: nuclear receptor coactivator 7
Synonyms: 9030406N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 211329
VEGA: 10
Homologene: 65245
Tm9sf4
Name: transmembrane 9 superfamily member 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99237
Homologene: 21620
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Tle1
Name: transducin-like enhancer of split 1
Synonyms: Estm14, C230057C06Rik, Grg1, Tle4l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21885
Homologene: 21058
Leo1
Name: Leo1, Paf1/RNA polymerase II complex component
Synonyms: LOC235497
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235497
VEGA: 9
Homologene: 133895
Smg1
Name: SMG1 nonsense mediated mRNA decay associated PI3K related kinase
Synonyms: 5430435M13Rik, SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans), C130002K18Rik, 2610207I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233789
Homologene: 56697
Zfp106
Name: zinc finger protein 106
Synonyms: Sh3bp3, H3a, Cd-1, sirm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20402
Homologene: 40787
Dcaf8
Name: DDB1 and CUL4 associated factor 8
Synonyms: Wdr42a, D1Dau35e, D1Ucla4, H326
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98193
Homologene: 56725
Rsf1
Name: remodeling and spacing factor 1
Synonyms: p325, Hbxap, C030033M12Rik, 4832420A03Rik, XAP8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233532
Homologene: 41142
Bicd1
Name: BICD cargo adaptor 1
Synonyms: B830009D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12121
HGNC: HGNC:1049
Homologene: 37518
Fh1
Name: fumarate hydratase 1
Synonyms: fumarase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14194
HGNC: HGNC:3700
Homologene: 115
Wdr26
Name: WD repeat domain 26
Synonyms: 1600024A01Rik, Gid7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226757
Homologene: 11857
Dhx32
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 32
Synonyms: Ddx32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101437
Homologene: 56798
Ubr4
Name: ubiquitin protein ligase E3 component n-recognin 4
Synonyms: LOC381562, D930005K06Rik, Zubr1, p600, A930005E13Rik, 1810009A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69116
Homologene: 10804
Clca4b
Name: chloride channel accessory 4B
Synonyms: AI747448
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99709
HGNC: HGNC:2018
Homologene: 40808
Adamts12
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239337
VEGA: 15
Homologene: 12808
Elac2
Name: elaC ribonuclease Z 2
Synonyms: D11Wsu80e, 1110017O07Rik, tRNase Z(L)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68626
Homologene: 6403
Slc39a6
Name: solute carrier family 39 (metal ion transporter), member 6
Synonyms: Zip6, Ermelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 106957
Homologene: 8199
Pcdh12
Name: protocadherin 12
Synonyms: VE-cadherin-2, vascular endothelial cadherin-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 53601
HGNC: HGNC:8657
Homologene: 9574
Col1a2
Name: collagen, type I, alpha 2
Synonyms: Cola2, Cola-2, Col1a-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12843
HGNC: HGNC:2198
Homologene: 69
Cacna2d2
Name: calcium channel, voltage-dependent, alpha 2/delta subunit 2
Synonyms: a2d2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56808
HGNC: HGNC:1400
Homologene: 4400
Trpm3
Name: transient receptor potential cation channel, subfamily M, member 3
Synonyms: 6330504P12Rik, melastatin 2, LTRPC3, MLSN2, B930001P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226025
VEGA: 19
Homologene: 62287
Gpr63
Name: G protein-coupled receptor 63
Synonyms: PSP24beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 81006
Homologene: 12759
Metrn
Name: meteorin, glial cell differentiation regulator
Synonyms: 1810034B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70083
VEGA: 17
Homologene: 11432
Fat4
Name: FAT atypical cadherin 4
Synonyms: 6030410K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329628
Homologene: 14377
Or8b37
Name: olfactory receptor family 8 subfamily B member 37
Synonyms: MOR162-9P, Olfr1550-ps1, Olfr884, MOR162-11P, MOR162-11P, MOR162-13, GA_x6K02T2PVTD-31726544-31727473
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 257996
Homologene: 79400
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Uroc1
Name: urocanase domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243537
Homologene: 76629
Map3k10
Name: mitogen-activated protein kinase kinase kinase 10
Synonyms: mixed lineage kinase 2, Mlk2, MKN28 kinase, serine/threonine kinase, MKN28 derived nonreceptor_type
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269881
HGNC: HGNC:6849
Homologene: 1834
Vmn2r70
Name: vomeronasal 2, receptor 70
Synonyms: EG620835
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 670940
Homologene: 115466
Mmp12
Name: matrix metallopeptidase 12
Synonyms: MMP12, macrophage elastase, Mmel
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17381
HGNC: HGNC:7158
Homologene: 20547
Gypa
Name: glycophorin A
Synonyms: CD235a, GPA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14934
Ankra2
Name: ankyrin repeat, family A (RFXANK-like), 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68558
Homologene: 11312
Gpr22
Name: G protein-coupled receptor 22
Synonyms: 2900068K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73010
HGNC: HGNC:4477
Homologene: 18420
Mtus2
Name: microtubule associated tumor suppressor candidate 2
Synonyms: C130038G02Rik, 5730592G18Rik, A730013O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 77521
Homologene: 78141
Shcbp1
Name: Shc SH2-domain binding protein 1
Synonyms: mPAL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20419
Homologene: 32123
4933427D14Rik
Name: RIKEN cDNA 4933427D14 gene
Synonyms: Gm43951
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74477
Homologene: 8874
Klhdc7b
Name: kelch domain containing 7B
Synonyms: EG546648
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 546648
Homologene: 34707
Nlrp4d
Name: NLR family, pyrin domain containing 4D
Synonyms: Nalp-beta, Nalp4d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384752
Capn13
Name: calpain 13
Synonyms: LOC381122
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381122
VEGA: 17
Homologene: 27026
Mss51
Name: MSS51 mitochondrial translational activator
Synonyms: 4833444M15Rik, Zmynd17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74843
VEGA: 14
Homologene: 27997
Sun1
Name: Sad1 and UNC84 domain containing 1
Synonyms: 5730434D03Rik, Unc84a, 4632417G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 77053
Homologene: 11544
Lrrc66
Name: leucine rich repeat containing 66
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231296
Homologene: 51944
Mamdc4
Name: MAM domain containing 4
Synonyms: LOC381352
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 381352
Homologene: 17102
Adgrb1
Name: adhesion G protein-coupled receptor B1
Synonyms: Bai1, B830018M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 107831
HGNC: HGNC:943
Homologene: 1287
Pex5l
Name: peroxisomal biogenesis factor 5-like
Synonyms: Pex2, 1700016J08Rik, PXR2, TRIP8b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 58869
Homologene: 9562
Lrrc74a
Name: leucine rich repeat containing 74A
Synonyms: Lrrc74, Gm6772
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 627607
VEGA: 12
Homologene: 19331
Ddx31
Name: DEAD/H box helicase 31
Synonyms: DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 31, 5830444G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227674
Homologene: 6389
Or5b97
Name: olfactory receptor family 5 subfamily B member 97
Synonyms: Olfr1447, MOR202-3, GA_x6K02T2RE5P-3231251-3230331
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258698
HGNC: HGNC:8324
Homologene: 74233
Calhm2
Name: calcium homeostasis modulator family member 2
Synonyms: 2810048G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 72691
Homologene: 9303
AC135640.2
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Acsm3
Name: acyl-CoA synthetase medium-chain family member 3
Synonyms: Sah, Sa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20216
Homologene: 74559
Crx
Name: cone-rod homeobox
Synonyms: Crx1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12951
HGNC: HGNC:2383
Homologene: 467
Higd1c
Name: HIG1 domain family, member 1C
Synonyms: LOC380975, Yghl1-4, HIG1-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 380975
Homologene: 105259
Zfpm1
Name: zinc finger protein, multitype 1
Synonyms: Friend of GATA-1, Fog1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 22761
Homologene: 7606
Cyp2c68
Name: cytochrome P450, family 2, subfamily c, polypeptide 68
Synonyms: 9030012A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 433247
Homologene: 74936
Casd1
Name: CAS1 domain containing 1
Synonyms: Cast1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 213819
Homologene: 11287
Zfp456
Name: zinc finger protein 456
Synonyms: Rslcan-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 408065
Homologene: 110878
Gm14137
Name: predicted gene 14137
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 623781
Homologene: 85847
Pla2g2e
Name: phospholipase A2, group IIE
Synonyms: mGIIEsPLA2s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 26970
Homologene: 8083
Gm10064
Name: predicted gene 10064
Synonyms: EG665452
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 676564
Rasa4
Name: RAS p21 protein activator 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 54153
Homologene: 5080
Blvrb
Name: biliverdin reductase B (flavin reductase (NADPH))
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233016
HGNC: HGNC:1063
Homologene: 573
Spg7
Name: SPG7, paraplegin matrix AAA peptidase subunit
Synonyms: Cmar, spastic paraplegia 7 homolog (human), paraplegin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234847
Homologene: 31133
Slc16a13
Name: solute carrier family 16 (monocarboxylic acid transporters), member 13
Synonyms: 1700007D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69309
Homologene: 71749
Sytl5
Name: synaptotagmin-like 5
Synonyms: ENSMUSG00000054453
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 236643
Homologene: 16345
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 172,172,654 bp
  • T to G, chromosome 1 at 175,614,817 bp
  • T to C, chromosome 1 at 181,185,905 bp
  • T to A, chromosome 2 at 25,570,036 bp
  • T to C, chromosome 2 at 28,874,174 bp
  • A to G, chromosome 2 at 119,175,353 bp
  • C to T, chromosome 2 at 120,555,248 bp
  • T to A, chromosome 2 at 153,191,145 bp
  • G to T, chromosome 3 at 32,954,536 bp
  • A to G, chromosome 3 at 39,000,295 bp
  • A to G, chromosome 3 at 144,928,350 bp
  • A to G, chromosome 4 at 25,007,480 bp
  • A to T, chromosome 4 at 72,126,442 bp
  • A to C, chromosome 4 at 138,880,735 bp
  • T to C, chromosome 4 at 139,288,977 bp
  • A to T, chromosome 4 at 139,485,320 bp
  • T to C, chromosome 5 at 73,608,414 bp
  • T to C, chromosome 5 at 122,697,521 bp
  • T to C, chromosome 5 at 136,102,070 bp
  • T to A, chromosome 5 at 139,237,864 bp
  • A to G, chromosome 5 at 148,077,287 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • T to C, chromosome 6 at 4,619,753 bp
  • T to A, chromosome 6 at 90,336,955 bp
  • T to C, chromosome 6 at 149,512,914 bp
  • A to T, chromosome 7 at 10,377,685 bp
  • A to G, chromosome 7 at 15,871,133 bp
  • A to G, chromosome 7 at 27,448,130 bp
  • G to A, chromosome 7 at 27,661,567 bp
  • T to C, chromosome 7 at 85,565,904 bp
  • C to T, chromosome 7 at 97,679,027 bp
  • T to C, chromosome 7 at 106,330,210 bp
  • C to A, chromosome 7 at 118,146,289 bp
  • T to C, chromosome 7 at 119,783,984 bp
  • G to A, chromosome 7 at 133,737,421 bp
  • T to A, chromosome 8 at 4,736,297 bp
  • T to A, chromosome 8 at 33,248,918 bp
  • C to T, chromosome 8 at 80,496,792 bp
  • G to A, chromosome 8 at 122,336,659 bp
  • A to G, chromosome 8 at 123,070,417 bp
  • C to T, chromosome 9 at 7,358,290 bp
  • G to T, chromosome 9 at 38,047,827 bp
  • A to G, chromosome 9 at 67,961,649 bp
  • G to A, chromosome 9 at 75,457,138 bp
  • A to G, chromosome 9 at 107,517,257 bp
  • G to A, chromosome 10 at 30,691,579 bp
  • A to T, chromosome 11 at 30,110,902 bp
  • A to G, chromosome 11 at 59,032,709 bp
  • C to A, chromosome 11 at 64,998,523 bp
  • G to A, chromosome 11 at 70,219,031 bp
  • C to A, chromosome 11 at 72,159,455 bp
  • A to G, chromosome 11 at 77,976,585 bp
  • T to A, chromosome 12 at 31,709,313 bp
  • C to T, chromosome 12 at 86,745,579 bp
  • G to A, chromosome 13 at 67,366,544 bp
  • A to G, chromosome 13 at 98,271,727 bp
  • A to T, chromosome 14 at 20,483,092 bp
  • A to T, chromosome 14 at 74,642,147 bp
  • G to A, chromosome 15 at 11,255,683 bp
  • T to C, chromosome 15 at 25,722,157 bp
  • A to G, chromosome 15 at 74,548,549 bp
  • C to T, chromosome 15 at 89,387,395 bp
  • T to A, chromosome 15 at 100,374,989 bp
  • C to T, chromosome 17 at 25,796,228 bp
  • A to G, chromosome 17 at 45,674,134 bp
  • C to T, chromosome 17 at 73,322,069 bp
  • T to C, chromosome 18 at 24,601,470 bp
  • A to G, chromosome 18 at 38,282,464 bp
  • T to C, chromosome 19 at 12,900,895 bp
  • T to A, chromosome 19 at 22,987,789 bp
  • T to C, chromosome 19 at 39,739,550 bp
  • C to A, chromosome 19 at 47,132,917 bp
  • A to G, chromosome X at 9,994,497 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0729 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038910-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.