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Strain Name:
C;B6-Tg(Smgb-TAg)8Mir/Mmucd
Stock Number:
000039-UCD
Citation ID:
RRID:MMRRC_000039-UCD
Other Names:
C;B6-Tg(Smgb-TAg)8Mir, C;B6-Tg(Smgb-TAg)8Mir/Mmcd

Strain Information

Smgb
Name: Neonatal submandibular gland protein B
Type: Gene
Species: Rattus norvegicus (norway rat)
Chromosome: 3
TAg
Name: Simian virus 40 T antigen (SV40Tag)
Type: Gene
Species: Virus
Chromosome:
Tg(Smgb-TAg)8Mir
Name: transgene insertion 8, Lily Mirels
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
-10.5 kb to +30 from the Smgb salivary protein gene upstream of SV40 T antigen.
Genotype Determination
Phenotype
Transgene is expressed in the intercalated duct cells of the submandibular gland. Expression results in progressive hyperplasia and dysplasia of intercalated ducts in all mice. The phenotype is much more pronounced in males than females. Submandibular adenocarcinoma develops in approximately half of all male mice by 12 months of age (and rarely, in females). Other submandibular gland cells, and the other major salivary glands, are unaffected. Some males have tumors at 6-8 months; others don't develop until a year and some just have severe dysplasia which is only detected histologically. Transgene is a multifunctional regulatory protein that stimulates gene transcription and forms complexes with cell cycle-regulatory proteins such as Trp53 and Rb1 that are implicated in human breast cancer.
MeSH Terms
  • Adenocarcinoma/pathology
  • Animals
  • Base Sequence
  • DNA Primers
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Transgenic
  • Reverse Transcriptase Polymerase Chain Reaction
  • Salivary Gland Neoplasms/pathology
  • Submandibular Gland/pathology
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
  • Alterations in gene expression between hyperplasia, dysplasia and adenocarcinoma.
  • Possible second site mutations involved in submandibular carcinogenesis.
Donor
Dr. L. Mirels, University of California, Berkeley
Primary Reference
Dardick I, Ho J, Paulus M, Mellon PL, Mirels L. Submandibular gland adenocarcinoma of intercalated duct origin in Smgb-Tag mice. Lab Invest. 2000 Nov;80(11):1657-70. (Medline PMID: 11092526)
Strain Development


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

MMRRC colony staff observe that these mice are jumpy and try to escape their cages.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Sib mating
Breeding Scheme(s)
  • Hemizygous female x Wildtype male sibling
  • Wildtype female x Hemizygous male sibling
Generation
Unknown
Overall Breeding Performance
Good. Note: homozygous mice are more difficult to breed.
Average litter size
Unknown
Recommended wean age
4 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000039-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,568.61
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000039-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $882.81
Non-Profit / For-Profit
Aliquot Approximate quantity3
000039-UCD-RESUS Litter recovered from cryo-archive $5,892.91 / $6,181.91
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.