Strain Name:
C57BL/6J-MtgxR0854Btlr/Mmmh
Stock Number:
039033-MU
Citation ID:
RRID:MMRRC_039033-MU
Other Names:
R0854 (G1), C57BL/6J-MtgxR0854Btlr
Major Collection:

Strain Information

Sema6d
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms: 1110067B02Rik, Sema6D-2, Sema6D-4, Sema6D-6, Sema6D-1, Sema6D-5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214968
Homologene: 16195
Gng7
Name: guanine nucleotide binding protein (G protein), gamma 7
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14708
HGNC: HGNC:4410
Homologene: 31293
Alox12b
Name: arachidonate 12-lipoxygenase, 12R type
Synonyms: e-LOX2, Aloxe2, 12R-LOX
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11686
HGNC: HGNC:430
Homologene: 884
Tsc22d1
Name: TSC22 domain family, member 1
Synonyms: Egr5, Tgfb1i4, TSC-22
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 21807
Homologene: 7573
Epm2aip1
Name: EPM2A interacting protein 1
Synonyms: A930003G21Rik, EPM2A (laforin) interacting protein 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 77781
Homologene: 8875
Sarnp
Name: SAP domain containing ribonucleoprotein
Synonyms: 1110005A23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66118
VEGA: 10
Homologene: 41628
Zranb1
Name: zinc finger, RAN-binding domain containing 1
Synonyms: D7Wsu87e, 9330160G10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 360216
Homologene: 9728
Vezf1
Name: vascular endothelial zinc finger 1
Synonyms: db1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22344
Homologene: 5175
Dgcr8
Name: DGCR8, microprocessor complex subunit
Synonyms: Gy1, N41, Vo59c07, D16H22S788E, D16H22S1742E, DiGeorge syndrome critical region gene 8, D16Wis2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 94223
HGNC: HGNC:2847
Homologene: 11223
Pask
Name: PAS domain containing serine/threonine kinase
Synonyms: Paskin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269224
Homologene: 9038
Nceh1
Name: neutral cholesterol ester hydrolase 1
Synonyms: B230106I24Rik, CPO-BP, mKIAA1363, Aadacl1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 320024
Homologene: 23251
Thsd1
Name: thrombospondin, type I, domain 1
Synonyms: Tmtsp, 4833423O18Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56229
Homologene: 10264
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
P3h3
Name: prolyl 3-hydroxylase 3
Synonyms: Grcb, Leprel2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14789
Homologene: 8401
Fer1l6
Name: fer-1 like family member 6
Synonyms: EG631797
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 631797
Homologene: 53396
Sec24c
Name: SEC24 homolog C, COPII coat complex component
Synonyms: 2610204K03Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218811
VEGA: 14
Homologene: 3615
Wdr17
Name: WD repeat domain 17
Synonyms: 3010002I12Rik, B230207L18Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244484
Homologene: 12460
Myh4
Name: myosin, heavy polypeptide 4, skeletal muscle
Synonyms: MyHC-IIb, Myhsf, MHC2B, Minmus, MYH-2B, Minimsc, MM
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17884
HGNC: HGNC:7574
Homologene: 123880
Mybpc2
Name: myosin binding protein C, fast-type
Synonyms: Fast-type C-protein
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233199
HGNC: HGNC:7550
Homologene: 3331
Notch4
Name: notch 4
Synonyms: Int-3, N4, Int3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18132
HGNC: HGNC:7884
Homologene: 3351
Vmn2r58
Name: vomeronasal 2, receptor 58
Synonyms: EG628422
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 628422
Cfap57
Name: cilia and flagella associated protein 57
Synonyms: C130004B06Rik, LOC384050, 1110020C03Rik, Wdr65
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68625
Homologene: 51350
Tnfsf18
Name: tumor necrosis factor (ligand) superfamily, member 18
Synonyms: GITR ligand, Gitrl
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240873
Homologene: 3732
Hbb-bh2
Name: hemoglobin beta, bh2
Synonyms: Gm5736
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 436003
Ddt
Name: D-dopachrome tautomerase
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13202
Homologene: 1038
Pgbd1
Name: piggyBac transposable element derived 1
Synonyms: 4921509E05Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 319207
Homologene: 130030
Ncan
Name: neurocan
Synonyms: Tgfbit, Cspg3, Cspg3-rs
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13004
HGNC: HGNC:2465
Homologene: 3229
Brox
Name: BRO1 domain and CAAX motif containing
Synonyms: 0610010K06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71678
Homologene: 13499
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 93,327,400 bp
  • T to C, chromosome 1 at 93,327,412 bp
  • T to A, chromosome 1 at 93,327,434 bp
  • T to A, chromosome 1 at 161,503,668 bp
  • T to G, chromosome 1 at 183,287,758 bp
  • C to T, chromosome 2 at 124,665,302 bp
  • T to A, chromosome 3 at 27,241,319 bp
  • G to A, chromosome 4 at 118,561,872 bp
  • G to T, chromosome 6 at 124,854,933 bp
  • T to G, chromosome 7 at 41,837,138 bp
  • T to C, chromosome 7 at 44,517,002 bp
  • T to C, chromosome 7 at 103,840,065 bp
  • T to C, chromosome 7 at 132,949,848 bp
  • G to A, chromosome 8 at 22,258,571 bp
  • C to T, chromosome 8 at 54,703,881 bp
  • G to T, chromosome 8 at 70,112,552 bp
  • T to A, chromosome 9 at 111,272,499 bp
  • C to T, chromosome 10 at 75,771,495 bp
  • A to G, chromosome 10 at 80,951,673 bp
  • T to A, chromosome 10 at 128,851,145 bp
  • T to C, chromosome 11 at 67,259,147 bp
  • G to A, chromosome 11 at 69,164,476 bp
  • A to T, chromosome 11 at 88,068,435 bp
  • T to A, chromosome 11 at 88,068,436 bp
  • A to G, chromosome 13 at 21,423,172 bp
  • A to G, chromosome 14 at 20,689,340 bp
  • C to T, chromosome 14 at 76,418,201 bp
  • A to G, chromosome 15 at 58,559,188 bp
  • G to A, chromosome 16 at 18,280,505 bp
  • A to T, chromosome 16 at 32,778,955 bp
  • T to A, chromosome 17 at 34,568,572 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0854 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039033-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.