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Strain Name:
C57BL/6J-MtgxR0919Btlr/Mmmh
Stock Number:
039069-MU
Citation ID:
RRID:MMRRC_039069-MU
Other Names:
R0919 (G1), C57BL/6J-MtgxR0919Btlr
Major Collection:

Strain Information

Sash1
Name: SAM and SH3 domain containing 1
Synonyms: A330076K04Rik, 1100001C18Rik, 2500002E12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70097
Homologene: 69182
U2af2
Name: U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22185
Homologene: 110853
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Sgsm1
Name: small G protein signaling modulator 1
Synonyms: 2410098H20Rik, D5Bwg1524e, Rutbc2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52850
Homologene: 64485
Kpna4
Name: karyopherin subunit alpha 4
Synonyms: IPOA3, 1110058D08Rik, importin alpha 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16649
HGNC: HGNC:6397
Homologene: 20521
Txn2
Name: thioredoxin 2
Synonyms: 2510006J11Rik, Trx2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 56551
Homologene: 40849
Washc2
Name: WASH complex subunit 2
Synonyms: C530005J20Rik, D6Wsu116e, Fam21
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28006
Homologene: 41686
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 10,577,571 bp
  • T to A, chromosome 2 at 76,946,742 bp
  • T to A, chromosome 2 at 82,985,484 bp
  • A to G, chromosome 2 at 86,008,640 bp
  • G to A, chromosome 2 at 126,831,238 bp
  • T to C, chromosome 2 at 128,617,731 bp
  • A to T, chromosome 3 at 69,085,828 bp
  • C to T, chromosome 3 at 96,651,738 bp
  • A to T, chromosome 3 at 100,071,943 bp
  • A to G, chromosome 3 at 154,243,586 bp
  • A to G, chromosome 4 at 10,882,462 bp
  • T to C, chromosome 5 at 52,855,637 bp
  • C to T, chromosome 5 at 113,258,842 bp
  • T to C, chromosome 5 at 136,197,831 bp
  • C to T, chromosome 6 at 116,208,264 bp
  • C to T, chromosome 7 at 5,069,434 bp
  • A to C, chromosome 7 at 16,201,958 bp
  • T to C, chromosome 7 at 67,735,347 bp
  • A to G, chromosome 7 at 103,460,812 bp
  • A to T, chromosome 7 at 104,975,312 bp
  • A to G, chromosome 8 at 3,158,769 bp
  • A to T, chromosome 9 at 107,655,959 bp
  • A to G, chromosome 10 at 8,730,079 bp
  • A to G, chromosome 10 at 18,589,735 bp
  • G to A, chromosome 11 at 97,056,101 bp
  • A to G, chromosome 11 at 102,309,777 bp
  • A to G, chromosome 12 at 74,299,000 bp
  • C to T, chromosome 13 at 56,822,649 bp
  • T to C, chromosome 13 at 105,444,836 bp
  • G to A, chromosome 14 at 20,404,164 bp
  • T to C, chromosome 15 at 77,927,749 bp
  • T to C, chromosome 16 at 5,064,391 bp
  • T to C, chromosome 16 at 15,667,575 bp
  • T to C, chromosome 17 at 3,197,120 bp
  • T to C, chromosome 17 at 23,610,007 bp
  • T to A, chromosome 17 at 34,059,150 bp
  • T to A, chromosome 17 at 47,672,451 bp
  • G to A, chromosome 17 at 86,630,160 bp
  • C to A, chromosome 18 at 65,307,473 bp
  • G to C, chromosome 18 at 84,949,262 bp
  • T to A, chromosome 19 at 4,222,198 bp
  • T to A, chromosome 19 at 36,990,743 bp
  • C to T, chromosome 19 at 38,914,049 bp
  • T to G, chromosome 19 at 39,404,669 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0919 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039069-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.