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Strain Name:
C57BL/6J-MtgxR0944Btlr/Mmmh
Stock Number:
039083-MU
Citation ID:
RRID:MMRRC_039083-MU
Other Names:
R0944 (G1), C57BL/6J-MtgxR0944Btlr
Major Collection:

Strain Information

Pmel
Name: premelanosome protein
Synonyms: Pmel17, gp100, gp87, D12S53Eh, D10H12S53E, Si
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20431
VEGA: 10
Homologene: 5048
Mindy3
Name: MINDY lysine 48 deubiquitinase 3
Synonyms: 1810041E18Rik, 5830410F13Rik, 2310047O13Rik, Fam188a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66960
Homologene: 11478
Sgsm1
Name: small G protein signaling modulator 1
Synonyms: 2410098H20Rik, D5Bwg1524e, Rutbc2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52850
Homologene: 64485
Slk
Name: STE20-like kinase
Synonyms: 9A2, mSLK, Etk4, SLK, Stk2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20874
Homologene: 22515
Eml4
Name: echinoderm microtubule associated protein like 4
Synonyms: 4930443C24Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 78798
VEGA: 17
HGNC: HGNC:1316
Homologene: 56841
Pdxdc1
Name: pyridoxal-dependent decarboxylase domain containing 1
Synonyms: 2210010A19Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 94184
Homologene: 22858
Usp25
Name: ubiquitin specific peptidase 25
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 30940
VEGA: 16
Homologene: 8374
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 14,912,361 bp
  • A to G, chromosome 1 at 74,279,522 bp
  • T to C, chromosome 2 at 12,396,182 bp
  • T to C, chromosome 2 at 36,890,686 bp
  • A to C, chromosome 2 at 86,828,937 bp
  • A to G, chromosome 2 at 129,006,816 bp
  • A to G, chromosome 2 at 130,397,503 bp
  • A to G, chromosome 4 at 41,629,997 bp
  • G to A, chromosome 4 at 46,029,762 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • A to G, chromosome 4 at 99,762,060 bp
  • G to A, chromosome 4 at 119,238,759 bp
  • C to A, chromosome 5 at 4,064,742 bp
  • A to G, chromosome 5 at 108,656,465 bp
  • G to A, chromosome 5 at 113,265,874 bp
  • C to A, chromosome 6 at 42,313,141 bp
  • T to C, chromosome 6 at 59,353,915 bp
  • T to C, chromosome 6 at 68,161,683 bp
  • A to G, chromosome 6 at 113,338,391 bp
  • T to C, chromosome 6 at 140,570,196 bp
  • T to A, chromosome 7 at 12,811,468 bp
  • T to A, chromosome 7 at 46,015,505 bp
  • T to C, chromosome 7 at 89,866,569 bp
  • C to T, chromosome 9 at 26,756,941 bp
  • A to T, chromosome 9 at 55,488,838 bp
  • T to C, chromosome 9 at 62,427,981 bp
  • A to T, chromosome 10 at 74,210,470 bp
  • G to C, chromosome 10 at 103,409,796 bp
  • A to T, chromosome 10 at 128,715,257 bp
  • A to G, chromosome 11 at 20,001,104 bp
  • A to G, chromosome 11 at 86,244,463 bp
  • T to G, chromosome 11 at 104,710,730 bp
  • G to A, chromosome 12 at 107,489,009 bp
  • T to C, chromosome 13 at 27,318,166 bp
  • T to C, chromosome 13 at 92,661,105 bp
  • A to G, chromosome 14 at 50,362,336 bp
  • A to G, chromosome 14 at 64,032,232 bp
  • C to G, chromosome 14 at 101,479,220 bp
  • T to C, chromosome 15 at 47,611,831 bp
  • T to C, chromosome 15 at 71,881,662 bp
  • A to G, chromosome 15 at 98,497,684 bp
  • A to G, chromosome 16 at 10,688,646 bp
  • A to G, chromosome 16 at 13,838,369 bp
  • A to G, chromosome 16 at 44,384,761 bp
  • T to C, chromosome 16 at 77,081,447 bp
  • T to C, chromosome 16 at 91,023,427 bp
  • T to C, chromosome 16 at 92,393,491 bp
  • T to A, chromosome 17 at 18,947,403 bp
  • A to T, chromosome 17 at 44,608,236 bp
  • G to A, chromosome 17 at 83,478,060 bp
  • T to C, chromosome 19 at 47,608,993 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0944 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039083-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.