Strain Name:
C57BL/6J-MtgxR0965Btlr/Mmmh
Stock Number:
039094-MU
Citation ID:
RRID:MMRRC_039094-MU
Other Names:
R0965 (G1), C57BL/6J-MtgxR0965Btlr
Major Collection:

Gene Information

Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, DNA-PK, XRCC7, DOXNPH, dxnph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Zc3h11a
Name: zinc finger CCCH type containing 11A
Synonyms: 1110003F06Rik, 5730454B08Rik, G630041M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 70579
Homologene: 8888
Brd1
Name: bromodomain containing 1
Synonyms: 1110059H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223770
HGNC: HGNC:1102
Homologene: 40956
Gtf3c3
Name: general transcription factor IIIC, polypeptide 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98488
HGNC: HGNC:4666
Homologene: 40805
Bcl2
Name: B cell leukemia/lymphoma 2
Synonyms: Bcl-2, C430015F12Rik, D830018M01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12043
HGNC: HGNC:990
Homologene: 527
Adgrb2
Name: adhesion G protein-coupled receptor B2
Synonyms: Bai2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230775
HGNC: HGNC:944
Homologene: 1288
Rsbn1l
Name: round spermatid basic protein 1-like
Synonyms: 8430412F05Rik, C330002G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 242860
Homologene: 19435
Med19
Name: mediator complex subunit 19
Synonyms: LCMR1, 2410018M14Rik, 3110040A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 381379
Homologene: 14684
Esco1
Name: establishment of sister chromatid cohesion N-acetyltransferase 1
Synonyms: A930014I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 77805
Homologene: 62166
Slc7a5
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
Synonyms: TA1, D0H16S474E, LAT1, Gm42049
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20539
Homologene: 55759
Tmem119
Name: transmembrane protein 119
Synonyms: obif
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231633
Homologene: 17145
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, D830007G01Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Mcc
Name: mutated in colorectal cancers
Synonyms: D18Ertd451e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 328949
HGNC: HGNC:6935
Homologene: 20539
Phf11c
Name: PHD finger protein 11C
Synonyms: Gm6907
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 628705
Homologene: 87807
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Muc5b
Name: mucin 5, subtype B, tracheobronchial
Synonyms: MUC9, MUC5, 2300002I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74180
HGNC: HGNC:7516
Homologene: 136756
Slco1b2
Name: solute carrier organic anion transporter family, member 1b2
Synonyms: mlst-1, Slc21a6, Slc21a10, Oatp1b2, 7330442B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 28253
Homologene: 75119
Sned1
Name: sushi, nidogen and EGF-like domains 1
Synonyms: 6720455I24Rik, Snep, D430044C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 208777
Homologene: 14708
Rbm20
Name: RNA binding motif protein 20
Synonyms: 2010003H22Rik, 1110018J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 73713
Homologene: 28386
Or9g4b
Name: olfactory receptor family 9 subfamily G member 4B
Synonyms: GA_x6K02T2Q125-47264151-47265089, MOR213-3, Olfr1015
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258564
Homologene: 115499
Nfatc4
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 4
Synonyms: 3110041H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 73181
VEGA: 14
HGNC: HGNC:7778
Homologene: 3349
Amacr
Name: alpha-methylacyl-CoA racemase
Synonyms: 2-arylpropionyl-CoA epimerase, Macr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17117
VEGA: 15
HGNC: HGNC:451
Homologene: 7410
Ammecr1l
Name: AMME chromosomal region gene 1-like
Synonyms: 5430429D03Rik, E230022H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225339
VEGA: 18
Homologene: 69488
Gm20537
Name: predicted gene 20537
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Gzma
Name: granzyme A
Synonyms: serine esterase 1, TSP1, BLT esterase, Hanukah factor, Ctla-3, Hf, Ctla3, H factor, SE1, TSP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14938
VEGA: 13
HGNC: HGNC:4708
Homologene: 21237
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 54,417,778 bp
  • G to A, chromosome 1 at 93,281,654 bp
  • A to T, chromosome 1 at 106,712,291 bp
  • T to C, chromosome 1 at 133,645,803 bp
  • C to A, chromosome 2 at 76,799,571 bp
  • A to G, chromosome 2 at 84,678,449 bp
  • A to G, chromosome 2 at 85,786,299 bp
  • AGAGGAGGAGGAGGAGGAGG to AGAGGAGGAGGAGGAGG, chromosome 4 at 129,992,416 bp
  • A to G, chromosome 5 at 20,908,248 bp
  • A to C, chromosome 5 at 113,795,419 bp
  • C to A, chromosome 6 at 141,685,596 bp
  • C to T, chromosome 7 at 141,863,802 bp
  • T to C, chromosome 8 at 121,907,101 bp
  • A to T, chromosome 9 at 88,454,307 bp
  • T to C, chromosome 11 at 59,131,646 bp
  • G to A, chromosome 13 at 113,098,334 bp
  • T to C, chromosome 14 at 55,826,586 bp
  • T to A, chromosome 14 at 59,381,482 bp
  • C to T, chromosome 15 at 10,984,805 bp
  • G to A, chromosome 15 at 88,717,028 bp
  • T to C, chromosome 16 at 15,829,716 bp
  • A to G, chromosome 18 at 10,567,570 bp
  • T to A, chromosome 18 at 31,774,226 bp
  • A to G, chromosome 18 at 44,724,526 bp
  • T to C, chromosome 19 at 53,859,401 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0965 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039094-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.