Strain Name:
C57BL/6J-MtgxR0966Btlr/Mmmh
Stock Number:
039095-MU
Citation ID:
RRID:MMRRC_039095-MU
Other Names:
R0966 (G1), C57BL/6J-MtgxR0966Btlr
Major Collection:

Gene Information

Polr3e
Name: polymerase (RNA) III (DNA directed) polypeptide E
Synonyms: RPC5, Sin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26939
Homologene: 14052
Arhgef10
Name: Rho guanine nucleotide exchange factor (GEF) 10
Synonyms: 6430549H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234094
Homologene: 22827
Igf2bp2
Name: insulin-like growth factor 2 mRNA binding protein 2
Synonyms: IMP-2, C330012H03Rik, IMP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 319765
Homologene: 4774
Prkca
Name: protein kinase C, alpha
Synonyms: Pkca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18750
HGNC: HGNC:9393
Homologene: 55679
Fzd8
Name: frizzled class receptor 8
Synonyms: Fz8, mFZ8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 14370
VEGA: 18
HGNC: HGNC:4046
Homologene: 40606
Wdr3
Name: WD repeat domain 3
Synonyms: D030020G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 269470
Homologene: 4937
Mmp16
Name: matrix metallopeptidase 16
Synonyms: Membrane type 3-MMP, MT3-MMP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17389
HGNC: HGNC:7162
Homologene: 55939
Smap1
Name: small ArfGAP 1
Synonyms: 4921514B13Rik, 1700056O10Rik, 4921525H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98366
Homologene: 134676
Flrt2
Name: fibronectin leucine rich transmembrane protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 399558
HGNC: HGNC:3761
Homologene: 8291
Slc5a2
Name: solute carrier family 5 (sodium/glucose cotransporter), member 2
Synonyms: Sglt2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 246787
Homologene: 2289
Hsh2d
Name: hematopoietic SH2 domain containing
Synonyms: ALX, Hsh2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 209488
Homologene: 13142
Myo7b
Name: myosin VIIB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17922
HGNC: HGNC:7607
Homologene: 81947
Cd101
Name: CD101 antigen
Synonyms: LOC381460, Igsf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 630146
HGNC: HGNC:5949
Homologene: 3142
Plekhh1
Name: pleckstrin homology domain containing, family H (with MyTH4 domain) member 1
Synonyms: D630024D12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 211945
VEGA: 12
Homologene: 121939
Ugt2b38
Name: UDP glucuronosyltransferase 2 family, polypeptide B38
Synonyms: 9430041C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100559
Homologene: 137225
2310061N02Rik
Name: RIKEN cDNA 2310061N02 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 69661
Homologene: 130759
Olfr68
Name: olfactory receptor 68
Synonyms: 3'[b]2, MOR22-3, GA_x6K02T2PBJ9-6504436-6503489
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18369
Homologene: 130658
Ugt1a6b
Name: UDP glucuronosyltransferase 1 family, polypeptide A6B
Synonyms: A9'
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 394435
Homologene: 85959
Vps36
Name: vacuolar protein sorting 36
Synonyms: 2810408E15Rik, 1700010A24Rik, Eap45, 2210415M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 70160
Homologene: 9374
Efcab5
Name: EF-hand calcium binding domain 5
Synonyms: 4930563A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319634
Homologene: 63366
Gm5724
Name: predicted gene 5724
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 435927
Slc22a13
Name: solute carrier family 22 (organic cation transporter), member 13
Synonyms: ORCTL3, OCTL3, OCTL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102570
VEGA: 9
HGNC: HGNC:8494
Homologene: 3140
Gm10110
Name: predicted gene 10110
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 23,876,368 bp
  • G to A, chromosome 1 at 88,107,128 bp
  • A to T, chromosome 3 at 100,161,069 bp
  • A to C, chromosome 3 at 101,008,222 bp
  • C to G, chromosome 4 at 18,115,930 bp
  • T to G, chromosome 5 at 87,412,373 bp
  • A to G, chromosome 6 at 141,727,573 bp
  • T to A, chromosome 7 at 103,777,449 bp
  • T to C, chromosome 7 at 120,926,937 bp
  • G to C, chromosome 7 at 128,270,631 bp
  • T to G, chromosome 8 at 14,940,343 bp
  • G to A, chromosome 8 at 22,206,817 bp
  • G to A, chromosome 8 at 72,200,460 bp
  • C to A, chromosome 9 at 119,196,477 bp
  • G to A, chromosome 11 at 77,140,923 bp
  • T to A, chromosome 11 at 108,014,284 bp
  • T to C, chromosome 12 at 79,065,730 bp
  • T to C, chromosome 12 at 95,780,301 bp
  • A to C, chromosome 14 at 89,898,119 bp
  • C to T, chromosome 16 at 22,089,090 bp
  • T to A, chromosome 16 at 88,707,422 bp
  • A to T, chromosome 18 at 9,214,745 bp
  • T to C, chromosome 18 at 31,998,763 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0966 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039095-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.