Strain Name:
C57BL/6J-MtgxR0991Btlr/Mmmh
Stock Number:
039111-MU
Citation ID:
RRID:MMRRC_039111-MU
Other Names:
R0991 (G1), C57BL/6J-MtgxR0991Btlr
Major Collection:

Gene Information

Foxp4
Name: forkhead box P4
Synonyms: 2310007G05Rik, 1200010K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74123
Homologene: 12536
U2af2
Name: U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22185
Homologene: 110853
Pik3c2a
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms: PI3KC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18704
HGNC: HGNC:8971
Homologene: 20581
Sf3b1
Name: splicing factor 3b, subunit 1
Synonyms: Targ4, SAP155, Prp10, SF3b155, 2810001M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 81898
Homologene: 6696
Xrn2
Name: 5'-3' exoribonuclease 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 24128
Homologene: 6927
Tenm2
Name: teneurin transmembrane protein 2
Synonyms: 9330187F13Rik, D3Bwg1534e, Ten-m2, 2610040L17Rik, Odz2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 23964
Homologene: 22672
Apc
Name: APC, WNT signaling pathway regulator
Synonyms: Min, adenomatosis polyposis coli, CC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 11789
HGNC: HGNC:583
Homologene: 30950
Dtwd1
Name: DTW domain containing 1
Synonyms: 1810033A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69185
Homologene: 10633
Nupl1
Name: nucleoporin like 1
Synonyms: 1700017F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71844
VEGA: 14
Homologene: 40924
Dna2
Name: DNA replication helicase/nuclease 2
Synonyms: Dna2l, E130315B21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 327762
HGNC: HGNC:2939
Homologene: 6124
Ltbp1
Name: latent transforming growth factor beta binding protein 1
Synonyms: 9430031G15Rik, Ltbp1L, LTBP-1, b2b1000Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268977
HGNC: HGNC:6714
Homologene: 522
Ccdc27
Name: coiled-coil domain containing 27
Synonyms: LOC381580
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381580
Homologene: 17596
Rd3
Name: retinal degeneration 3
Synonyms: rd3, 3322402L07Rik, rd-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74023
Homologene: 11373
Atp8a2
Name: ATPase, aminophospholipid transporter-like, class I, type 8A, member 2
Synonyms: Ib, wl, agil
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50769
Homologene: 4443
Edil3
Name: EGF-like repeats and discoidin I-like domains 3
Synonyms: Del1, Del-1, developmental endothelial locus-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13612
HGNC: HGNC:3173
Homologene: 21166
Vmn1r65
Name: vomeronasal 1 receptor 65
Synonyms: V3R6, V1rd6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 81013
Homologene: 110799
Mia
Name: melanoma inhibitory activity
Synonyms: Cdrap, Mia1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12587
HGNC: HGNC:7076
Homologene: 4763
Prl2c2
Name: prolactin family 2, subfamily c, member 2
Synonyms: Plf, PLF-1, MRP-1, Plf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18811
VEGA: 13
Homologene: 40763
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to G, chromosome 1 at 55,019,395 bp
  • A to G, chromosome 1 at 191,985,238 bp
  • G to A, chromosome 2 at 126,160,020 bp
  • A to G, chromosome 2 at 147,042,082 bp
  • G to A, chromosome 4 at 154,039,494 bp
  • A to G, chromosome 7 at 5,069,431 bp
  • G to A, chromosome 7 at 6,009,077 bp
  • G to A, chromosome 7 at 27,180,720 bp
  • A to G, chromosome 7 at 116,362,045 bp
  • C to T, chromosome 10 at 62,949,187 bp
  • C to T, chromosome 11 at 36,063,177 bp
  • G to C, chromosome 13 at 13,002,201 bp
  • A to T, chromosome 13 at 89,289,506 bp
  • T to C, chromosome 14 at 59,793,929 bp
  • A to T, chromosome 14 at 60,244,670 bp
  • G to C, chromosome 17 at 47,880,353 bp
  • A to G, chromosome 17 at 75,225,285 bp
  • T to A, chromosome 18 at 34,316,107 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0991 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039111-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.