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Strain Name:
C57BL/6J-MtgxR1147Btlr/Mmmh
Stock Number:
039220-MU
Citation ID:
RRID:MMRRC_039220-MU
Other Names:
R1147 (G1), C57BL/6J-MtgxR1147Btlr
Major Collection:

Strain Information

Arhgef12
Name: Rho guanine nucleotide exchange factor 12
Synonyms: LARG, 2310014B11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Zfat
Name: zinc finger and AT hook domain containing
Synonyms: LOC380993, Zfp406, Zfat1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 380993
Homologene: 16829
Trrap
Name: transformation/transcription domain-associated protein
Synonyms: transactivation/transformation-domain associated protein
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100683
Homologene: 39246
Zfp106
Name: zinc finger protein 106
Synonyms: Cd-1, H3a, sirm, Sh3bp3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20402
Homologene: 40787
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: 4930400K19Rik, Tulip1, 2310003F20Rik, Garnl1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Ash1l
Name: ASH1 like histone lysine methyltransferase
Synonyms: chromatin remodeling factor, 8030453L17Rik, E430018P19Rik, KMT2H
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 192195
Homologene: 10225
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 45,376,771 bp
  • A to G, chromosome 1 at 46,340,266 bp
  • A to T, chromosome 1 at 58,669,463 bp
  • C to G, chromosome 1 at 85,099,505 bp
  • T to C, chromosome 1 at 111,862,209 bp
  • A to G, chromosome 2 at 29,787,632 bp
  • A to T, chromosome 2 at 120,520,536 bp
  • C to T, chromosome 2 at 160,990,271 bp
  • C to T, chromosome 2 at 181,195,797 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • A to T, chromosome 3 at 88,984,887 bp
  • T to A, chromosome 3 at 108,752,541 bp
  • C to T, chromosome 3 at 122,794,313 bp
  • A to T, chromosome 4 at 6,823,055 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • A to G, chromosome 4 at 144,158,412 bp
  • T to A, chromosome 5 at 24,324,387 bp
  • T to C, chromosome 5 at 34,851,252 bp
  • T to G, chromosome 5 at 38,126,923 bp
  • T to A, chromosome 5 at 109,293,206 bp
  • G to A, chromosome 5 at 144,804,766 bp
  • A to T, chromosome 6 at 43,217,212 bp
  • T to A, chromosome 6 at 114,244,870 bp
  • T to A, chromosome 6 at 137,443,594 bp
  • C to T, chromosome 7 at 7,554,145 bp
  • A to T, chromosome 7 at 10,388,717 bp
  • G to A, chromosome 7 at 16,183,776 bp
  • A to G, chromosome 7 at 19,375,847 bp
  • G to A, chromosome 7 at 105,369,277 bp
  • T to A, chromosome 7 at 126,411,045 bp
  • T to C, chromosome 8 at 40,795,618 bp
  • A to G, chromosome 8 at 45,944,084 bp
  • C to A, chromosome 8 at 71,482,017 bp
  • C to T, chromosome 8 at 93,258,281 bp
  • T to C, chromosome 8 at 95,137,918 bp
  • C to T, chromosome 9 at 43,044,256 bp
  • CCTCTC to CCTCTCTC, chromosome 11 at 3,177,419 bp
  • T to C, chromosome 11 at 94,544,140 bp
  • A to T, chromosome 12 at 55,702,480 bp
  • T to G, chromosome 12 at 110,941,438 bp
  • A to G, chromosome 13 at 94,224,886 bp
  • A to G, chromosome 15 at 44,537,441 bp
  • A to T, chromosome 15 at 44,746,255 bp
  • A to T, chromosome 15 at 68,212,583 bp
  • G to T, chromosome 16 at 22,961,004 bp
  • G to A, chromosome 16 at 42,174,136 bp
  • A to T, chromosome 17 at 23,311,063 bp
  • T to C, chromosome 17 at 56,423,504 bp
  • G to A, chromosome 18 at 35,654,806 bp
  • G to A, chromosome 18 at 60,916,651 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1147 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039220-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.