Strain Name:
C57BL/6J-MtgxR1169Btlr/Mmmh
Stock Number:
039242-MU
Citation ID:
RRID:MMRRC_039242-MU
Other Names:
R1169 (G1), C57BL/6J-MtgxR1169Btlr
Major Collection:

Gene Information

Gene Symbol: Rptor [MGI:1921620] (Mus musculus (mouse))
Name: regulatory associated protein of MTOR, complex 1
Synonyms: Rap, 4932417H02Rik, raptor
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Sbf2 [MGI:1921831] (Mus musculus (mouse))
Name: SET binding factor 2
Synonyms: SBF2, mMTMH1, B430219L04Rik, 4833411B01Rik, Mtmr13
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Ptprz1 [MGI:97816] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, receptor type Z, polypeptide 1
Synonyms: RPTPz, Ptpz, Ptprz, Rptpbeta, DSD-1-PG, PTPbeta, phosphacan, PTPzeta
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Sim1 [MGI:98306] (Mus musculus (mouse))
Name: single-minded family bHLH transcription factor 1
Synonyms: bHLHe14
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Gli1 [MGI:95727] (Mus musculus (mouse))
Name: GLI-Kruppel family member GLI1
Synonyms: Zfp-5, Zfp5
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Plg [MGI:97620] (Mus musculus (mouse))
Name: plasminogen
Synonyms: Pg
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Lrp2 [MGI:95794] (Mus musculus (mouse))
Name: low density lipoprotein receptor-related protein 2
Synonyms: b2b1625.2Clo, Megalin, D230004K18Rik, Gp330
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 21,350,271 bp
  • A to T, chromosome 1 at 90,822,014 bp
  • A to G, chromosome 1 at 106,999,048 bp
  • G to T, chromosome 1 at 135,455,205 bp
  • T to C, chromosome 1 at 186,114,334 bp
  • T to C, chromosome 2 at 3,498,124 bp
  • A to G, chromosome 2 at 23,256,982 bp
  • T to A, chromosome 2 at 24,966,571 bp
  • A to T, chromosome 2 at 69,507,892 bp
  • A to G, chromosome 2 at 86,455,587 bp
  • A to G, chromosome 2 at 87,021,717 bp
  • G to A, chromosome 2 at 112,733,014 bp
  • A to T, chromosome 2 at 131,074,827 bp
  • A to T, chromosome 3 at 38,454,252 bp
  • A to T, chromosome 3 at 53,013,844 bp
  • A to G, chromosome 3 at 55,968,323 bp
  • G to A, chromosome 4 at 63,357,848 bp
  • T to A, chromosome 4 at 111,928,513 bp
  • C to T, chromosome 4 at 116,574,366 bp
  • T to G, chromosome 4 at 135,705,108 bp
  • T to C, chromosome 4 at 148,155,976 bp
  • C to A, chromosome 5 at 36,027,925 bp
  • A to T, chromosome 5 at 88,503,258 bp
  • A to T, chromosome 5 at 92,298,233 bp
  • T to C, chromosome 5 at 113,279,485 bp
  • GGGAGGAGGAGGAGGAGGAGGA to GGGAGGAGGAGGAGGAGGA, chromosome 5 at 123,254,610 bp
  • T to A, chromosome 5 at 134,492,250 bp
  • T to C, chromosome 5 at 137,311,920 bp
  • A to G, chromosome 5 at 142,762,984 bp
  • A to T, chromosome 6 at 22,965,712 bp
  • T to A, chromosome 6 at 55,494,116 bp
  • A to G, chromosome 6 at 83,032,048 bp
  • C to G, chromosome 6 at 115,932,238 bp
  • C to T, chromosome 6 at 128,674,795 bp
  • G to T, chromosome 7 at 68,165,127 bp
  • A to C, chromosome 7 at 82,635,171 bp
  • A to T, chromosome 7 at 85,751,309 bp
  • A to T, chromosome 7 at 110,310,184 bp
  • G to A, chromosome 7 at 118,551,260 bp
  • G to A, chromosome 7 at 131,074,524 bp
  • G to T, chromosome 7 at 135,667,612 bp
  • A to T, chromosome 7 at 139,983,929 bp
  • G to A, chromosome 7 at 140,126,331 bp
  • A to G, chromosome 7 at 140,778,207 bp
  • T to C, chromosome 8 at 3,603,866 bp
  • A to G, chromosome 8 at 22,335,473 bp
  • C to A, chromosome 8 at 71,580,653 bp
  • T to C, chromosome 9 at 5,299,454 bp
  • T to C, chromosome 9 at 20,171,058 bp
  • A to G, chromosome 9 at 31,150,985 bp
  • T to A, chromosome 9 at 49,274,226 bp
  • T to C, chromosome 9 at 57,609,995 bp
  • T to A, chromosome 9 at 57,612,564 bp
  • T to A, chromosome 9 at 70,746,292 bp
  • A to G, chromosome 9 at 105,896,974 bp
  • T to A, chromosome 10 at 25,994,348 bp
  • T to A, chromosome 10 at 50,981,522 bp
  • T to A, chromosome 10 at 77,900,930 bp
  • T to C, chromosome 10 at 80,583,947 bp
  • T to A, chromosome 10 at 127,338,451 bp
  • T to G, chromosome 10 at 128,323,596 bp
  • T to C, chromosome 11 at 60,521,771 bp
  • T to G, chromosome 11 at 68,762,841 bp
  • T to C, chromosome 11 at 87,817,317 bp
  • T to G, chromosome 11 at 100,263,345 bp
  • T to C, chromosome 11 at 119,895,890 bp
  • A to G, chromosome 12 at 4,639,694 bp
  • A to G, chromosome 12 at 24,578,011 bp
  • G to A, chromosome 12 at 65,143,283 bp
  • G to A, chromosome 12 at 71,075,338 bp
  • A to T, chromosome 12 at 113,285,952 bp
  • A to T, chromosome 12 at 115,808,455 bp
  • C to A, chromosome 13 at 27,606,904 bp
  • G to A, chromosome 13 at 74,150,743 bp
  • G to C, chromosome 13 at 91,685,271 bp
  • C to A, chromosome 13 at 109,950,928 bp
  • T to C, chromosome 14 at 14,095,468 bp
  • A to G, chromosome 14 at 56,514,165 bp
  • T to A, chromosome 15 at 4,091,226 bp
  • A to T, chromosome 16 at 22,078,730 bp
  • T to A, chromosome 17 at 12,384,512 bp
  • T to A, chromosome 17 at 15,735,732 bp
  • T to A, chromosome 17 at 34,333,357 bp
  • A to T, chromosome 17 at 34,742,972 bp
  • T to A, chromosome 17 at 35,083,264 bp
  • A to T, chromosome 19 at 10,054,099 bp
  • C to T, chromosome 19 at 12,109,489 bp
  • A to G, chromosome 19 at 58,734,951 bp
  • G to A, chromosome 19 at 60,580,408 bp
  • A to G, chromosome X at 152,144,731 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1169 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039242-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.