Strain Name:
C57BL/6J-MtgxR1234Btlr/Mmmh
Stock Number:
039302-MU
Citation ID:
RRID:MMRRC_039302-MU
Other Names:
R1234 (G1), C57BL/6J-MtgxR1234Btlr
Major Collection:

Strain Information

Xrcc1
Name: X-ray repair complementing defective repair in Chinese hamster cells 1
Synonyms: Xrcc-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22594
Homologene: 31368
Ccn1
Name: cellular communication network factor 1
Synonyms: CCN1, Cyr61
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16007
HGNC: HGNC:2654
Homologene: 1194
Nckap1
Name: NCK-associated protein 1
Synonyms: mh19, Hem-2, H19, Nap1, Hem2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 50884
HGNC: HGNC:7666
Homologene: 8384
Samd4b
Name: sterile alpha motif domain containing 4B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233033
Homologene: 35268
G3bp1
Name: GTPase activating protein (SH3 domain) binding protein 1
Synonyms: GAP SH3 binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27041
Homologene: 38096
Hk2
Name: hexokinase 2
Synonyms: HKII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15277
HGNC: HGNC:4923
Homologene: 37273
Snrnp40
Name: small nuclear ribonucleoprotein 40 (U5)
Synonyms: 0610009C03Rik, Wdr57
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66585
Homologene: 3538
M1ap
Name: meiosis 1 associated protein
Synonyms: D6Mm5e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 110958
Homologene: 13210
Dnajc13
Name: DnaJ heat shock protein family (Hsp40) member C13
Synonyms: Rme8, LOC382100, D030002L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235567
Homologene: 13574
Hmcn1
Name: hemicentin 1
Synonyms: LOC240793, EG545370
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545370
Homologene: 23741
Mapkapk2
Name: MAP kinase-activated protein kinase 2
Synonyms: MAPKAP kinase 2, Rps6kc1, MK2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17164
HGNC: HGNC:6887
Homologene: 56412
Fhod1
Name: formin homology 2 domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234686
Homologene: 136406
Dclk1
Name: doublecortin-like kinase 1
Synonyms: CPG16, DCLK, 1700113D08Rik, 2810480F11Rik, Dcl, Click-I, Dcamkl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 13175
HGNC: HGNC:2700
Homologene: 130530
Stab1
Name: stabilin 1
Synonyms: MS-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 192187
Homologene: 9035
Vars2
Name: valyl-tRNA synthetase 2, mitochondrial
Synonyms: 1190004I24Rik, Vars2l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 68915
Homologene: 57502
Cd300e
Name: CD300E molecule
Synonyms: Clm2, Trem5, Cd300le
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217306
Homologene: 18145
Vmn2r79
Name: vomeronasal 2, receptor 79
Synonyms: EG621430
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 621430
Homologene: 115466
Ptk6
Name: PTK6 protein tyrosine kinase 6
Synonyms: Sik, Tksk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20459
HGNC: HGNC:9617
Homologene: 68494
Ssrp1
Name: structure specific recognition protein 1
Synonyms: T160, Hmgi-rs3, Hmgox, Hmg1-rs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20833
Homologene: 110735
Psg18
Name: pregnancy specific beta-1-glycoprotein 18
Synonyms: mmCGM6, Cea-3, Cea3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26438
HGNC: HGNC:1816
Homologene: 110989
Tcf23
Name: transcription factor 23
Synonyms: Out, 2010002O16Rik, bHLHa24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69852
Homologene: 76449
Efhb
Name: EF hand domain family, member B
Synonyms: 4921525D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 211482
VEGA: 17
Homologene: 16982
Insyn1
Name: inhibitory synaptic factor 1
Synonyms: 6030419C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319477
VEGA: 9
Homologene: 18538
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 131,055,776 bp
  • G to A, chromosome 1 at 150,753,654 bp
  • C to T, chromosome 2 at 80,517,942 bp
  • T to C, chromosome 2 at 85,042,263 bp
  • C to T, chromosome 2 at 181,202,440 bp
  • T to C, chromosome 3 at 55,489,877 bp
  • A to G, chromosome 3 at 145,649,839 bp
  • C to G, chromosome 4 at 130,378,043 bp
  • T to C, chromosome 5 at 30,970,222 bp
  • G to A, chromosome 6 at 82,760,248 bp
  • A to G, chromosome 6 at 82,981,988 bp
  • A to T, chromosome 7 at 18,349,190 bp
  • T to C, chromosome 7 at 24,567,845 bp
  • C to T, chromosome 7 at 28,414,010 bp
  • A to T, chromosome 7 at 87,004,099 bp
  • T to C, chromosome 8 at 105,337,163 bp
  • AGAGGAGGAGGAGGAGG to AGAGGAGGAGGAGG, chromosome 9 at 58,499,432 bp
  • T to C, chromosome 9 at 104,214,157 bp
  • T to C, chromosome 11 at 55,489,202 bp
  • T to A, chromosome 11 at 115,055,366 bp
  • A to G, chromosome 14 at 31,150,236 bp
  • T to C, chromosome 17 at 35,667,146 bp
  • A to T, chromosome 17 at 53,451,587 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1234 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039302-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.