Strain Name:
C57BL/6J-MtgxR1243Btlr/Mmmh
Stock Number:
039310-MU
Citation ID:
RRID:MMRRC_039310-MU
Other Names:
R1243 (G1), C57BL/6J-MtgxR1243Btlr
Major Collection:

Gene Information

Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 77569
Homologene: 18953
Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, Nos-1, bNOS, NO, 2310005C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
Epb41l2
Name: erythrocyte membrane protein band 4.1 like 2
Synonyms: D10Ertd398e, 4.1G, Epb4.1l2, NBL2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 13822
VEGA: 10
HGNC: HGNC:3379
Homologene: 37478
Relch
Name: RAB11 binding and LisH domain, coiled-coil and HEAT repeat containing
Synonyms: 2310035C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 227446
Homologene: 10834
Ppm1g
Name: protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
Synonyms: Fin13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 14208
HGNC: HGNC:9278
Homologene: 31106
Pdia4
Name: protein disulfide isomerase associated 4
Synonyms: U48620, ERp72, Erp72, Cai
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 12304
Homologene: 21020
Dync2h1
Name: dynein cytoplasmic 2 heavy chain 1
Synonyms: 4432416O06Rik, DHC2, b2b414Clo, Dnchc2, m407Asp, m152Asp, D030010H02Rik, D330044F14Rik, DHC1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 110350
HGNC: HGNC:2962
Homologene: 14468
Casp8ap2
Name: caspase 8 associated protein 2
Synonyms: FLASH, D4Ertd659e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 26885
HGNC: HGNC:1510
Homologene: 8066
Cpsf3
Name: cleavage and polyadenylation specificity factor 3
Synonyms: 73 kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 54451
VEGA: 12
HGNC: HGNC:2326
Homologene: 6499
Gemin5
Name: gem nuclear organelle associated protein 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216766
Homologene: 9155
Myof
Name: myoferlin
Synonyms: E030042N20Rik, Fer1l3, 2310051D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 226101
VEGA: 19
HGNC: HGNC:3656
Homologene: 40882
Trpm3
Name: transient receptor potential cation channel, subfamily M, member 3
Synonyms: LTRPC3, 6330504P12Rik, MLSN2, melastatin 2, B930001P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 226025
VEGA: 19
Homologene: 62287
Myh3
Name: myosin, heavy polypeptide 3, skeletal muscle, embryonic
Synonyms: Myhse, Myhs-e, MyHC-emb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 17883
HGNC: HGNC:7573
Homologene: 20553
Pappa2
Name: pappalysin 2
Synonyms: PLAC3, placenta-specific 3, pregnancy-associated plasma preproprotein-A2, pregnancy-associated plasma protein-E, Pappe, PAPP-A2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 23850
Homologene: 10661
Disp2
Name: dispatched RND tramsporter family member 2
Synonyms: DispB, B230210L08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 214240
Homologene: 24916
Mus81
Name: MUS81 structure-specific endonuclease subunit
Synonyms: 1200008A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 71711
Homologene: 5725
Vmn2r98
Name: vomeronasal 2, receptor 98
Synonyms: EG224552
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224552
Homologene: 115024
Olfr992
Name: olfactory receptor 992
Synonyms: MOR203-1, GA_x6K02T2Q125-46877170-46876241
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258865
Homologene: 121517
Gm20605
Name: predicted gene 20605
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100316903
Slc34a1
Name: solute carrier family 34 (sodium phosphate), member 1
Synonyms: Slc17a2, renal Na+/Pi transporter, NaPi-IIa, Na/Pi cotransporter, Npt2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20505
VEGA: 13
Homologene: 20663
Zfp956
Name: zinc finger protein 956
Synonyms: AI894139
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 101197
Homologene: 128449
Cyp24a1
Name: cytochrome P450, family 24, subfamily a, polypeptide 1
Synonyms: Cyp24, 25-hydroxyvitamin D-24-hydroxylase, CP24, 24-OHase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 13081
HGNC: HGNC:2602
Homologene: 68094
Ajm1
Name: apical junction component 1
Synonyms: LOC381353, Gm996
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 381353
Homologene: 19943
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 105,750,364 bp
  • T to A, chromosome 1 at 158,845,100 bp
  • C to T, chromosome 2 at 25,578,558 bp
  • T to C, chromosome 2 at 85,400,273 bp
  • C to T, chromosome 2 at 118,791,822 bp
  • T to C, chromosome 2 at 170,495,406 bp
  • A to G, chromosome 4 at 32,645,687 bp
  • T to C, chromosome 5 at 31,208,111 bp
  • TTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC to TTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC, chromosome 5 at 66,998,597 bp
  • A to G, chromosome 5 at 117,905,472 bp
  • C to A, chromosome 5 at 137,642,091 bp
  • C to T, chromosome 6 at 47,807,120 bp
  • A to G, chromosome 6 at 47,956,051 bp
  • A to G, chromosome 9 at 7,120,882 bp
  • A to G, chromosome 10 at 25,489,043 bp
  • T to C, chromosome 11 at 58,139,488 bp
  • T to C, chromosome 11 at 67,090,453 bp
  • A to G, chromosome 12 at 21,307,980 bp
  • G to A, chromosome 13 at 55,412,131 bp
  • A to T, chromosome 17 at 19,065,948 bp
  • G to C, chromosome 19 at 5,485,117 bp
  • G to A, chromosome 19 at 22,698,722 bp
  • A to G, chromosome 19 at 37,936,092 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1243 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039310-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.