Strain Name:
C57BL/6J-MtgxR1254Btlr/Mmmh
Stock Number:
039321-MU
Citation ID:
RRID:MMRRC_039321-MU
Other Names:
R1254 (G1), C57BL/6J-MtgxR1254Btlr
Major Collection:

Gene Information

Ptk2
Name: PTK2 protein tyrosine kinase 2
Synonyms: FAK, FRNK, Fadk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 14083
VEGA: 15
HGNC: HGNC:9611
Homologene: 7314
Jarid2
Name: jumonji, AT rich interactive domain 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Ints6
Name: integrator complex subunit 6
Synonyms: Ddx26, DICE1, 2900075H24Rik, Notch2l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121
Kntc1
Name: kinetochore associated 1
Synonyms: jgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208628
Homologene: 32227
Etl4
Name: enhancer trap locus 4
Synonyms: E330027G05Rik, Sickle tail, Etl-4, 9430077C05Rik, Skt, 6620402G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 208618
Homologene: 10477
Rps6ka5
Name: ribosomal protein S6 kinase, polypeptide 5
Synonyms: 3110005L17Rik, 6330404E13Rik, MSK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73086
VEGA: 12
Homologene: 48302
Pcdhb16
Name: protocadherin beta 16
Synonyms: PcdhbP, Pcdhb8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93887
HGNC: HGNC:8693
Homologene: 99843
Robo4
Name: roundabout guidance receptor 4
Synonyms: 1200012D01Rik, Magic roundabout
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74144
Homologene: 10397
Cubn
Name: cubilin (intrinsic factor-cobalamin receptor)
Synonyms: D2Wsu88e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Cyp4a10
Name: cytochrome P450, family 4, subfamily a, polypeptide 10
Synonyms: Msp-3, D4Rp1, Cyp4a, RP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13117
HGNC: HGNC:2642
Homologene: 128044
Sstr1
Name: somatostatin receptor 1
Synonyms: Smstr1, sst1, Smstr-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20605
Homologene: 820
D430041D05Rik
Name: RIKEN cDNA D430041D05 gene
Synonyms: G2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241589
Homologene: 115928
Ccdc39
Name: coiled-coil domain containing 39
Synonyms: b2b1735Clo, 4921507O14Rik, D3Ertd789e, b2b1304Clo, prh, b2b2025.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 51938
Homologene: 12149
Duox2
Name: dual oxidase 2
Synonyms: A430065P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 214593
Homologene: 9689
Phactr1
Name: phosphatase and actin regulator 1
Synonyms: Rpel1, 9630030F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218194
Homologene: 33597
Tmem147
Name: transmembrane protein 147
Synonyms: 2010004E11Rik, 5033425B17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69804
Homologene: 12330
Plekha6
Name: pleckstrin homology domain containing, family A member 6
Synonyms: Pepp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240753
Homologene: 135779
Abcb10
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 10
Synonyms: ABC-me
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 56199
HGNC: HGNC:41
Homologene: 6474
Hivep3
Name: human immunodeficiency virus type I enhancer binding protein 3
Synonyms: E030045D18Rik, Krc, Schnurri-3, Shn3, 2900056N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16656
Homologene: 7803
Pank1
Name: pantothenate kinase 1
Synonyms: Pank1b, 4632412I06Rik, Pank1, 5430426F23Rik, Pank1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 75735
VEGA: 19
HGNC: HGNC:8598
Homologene: 56979
Coro2b
Name: coronin, actin binding protein, 2B
Synonyms: E130012P22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235431
HGNC: HGNC:2256
Homologene: 21228
Vmn1r7
Name: vomeronasal 1 receptor 7
Synonyms: Gm5568, V1rc31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 434016
Homologene: 83427
Magohb
Name: mago homolog B, exon junction complex core component
Synonyms: 2010012C16Rik, Magoh-rs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66441
Homologene: 56794
Rnf151
Name: ring finger protein 151
Synonyms: 1700010O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 67504
VEGA: 17
Homologene: 12146
Olfr477
Name: olfactory receptor 477
Synonyms: MOR204-1, GA_x6K02T2PBJ9-10319672-10320604
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258928
Homologene: 27246
Klhdc3
Name: kelch domain containing 3
Synonyms: Peas, 1300011D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 71765
VEGA: 17
Homologene: 14290
Rnase10
Name: ribonuclease, RNase A family, 10 (non-active)
Synonyms: 4930474F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 75019
VEGA: 14
Homologene: 19166
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 133,272,589 bp
  • A to T, chromosome 2 at 13,362,190 bp
  • A to G, chromosome 2 at 20,807,923 bp
  • T to G, chromosome 2 at 104,201,303 bp
  • T to A, chromosome 2 at 122,283,478 bp
  • T to C, chromosome 3 at 33,826,480 bp
  • G to A, chromosome 4 at 115,518,523 bp
  • A to G, chromosome 4 at 120,099,293 bp
  • G to T, chromosome 5 at 123,758,431 bp
  • A to G, chromosome 6 at 57,024,787 bp
  • T to C, chromosome 6 at 131,288,021 bp
  • A to T, chromosome 7 at 30,729,370 bp
  • T to A, chromosome 7 at 107,990,440 bp
  • C to T, chromosome 8 at 123,962,052 bp
  • T to C, chromosome 9 at 37,410,840 bp
  • A to G, chromosome 9 at 62,428,965 bp
  • G to A, chromosome 12 at 58,213,322 bp
  • G to T, chromosome 12 at 100,619,529 bp
  • T to A, chromosome 13 at 43,122,989 bp
  • T to A, chromosome 13 at 44,906,276 bp
  • A to T, chromosome 14 at 51,009,626 bp
  • A to G, chromosome 14 at 62,716,374 bp
  • C to T, chromosome 15 at 73,229,970 bp
  • A to T, chromosome 17 at 24,717,552 bp
  • C to T, chromosome 17 at 46,678,067 bp
  • T to G, chromosome 18 at 37,479,295 bp
  • T to C, chromosome 19 at 34,840,860 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1254 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039321-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.