Strain Name:
C57BL/6J-MtgxR1257Btlr/Mmmh
Stock Number:
039324-MU
Citation ID:
RRID:MMRRC_039324-MU
Other Names:
R1257 (G1), C57BL/6J-MtgxR1257Btlr
Major Collection:

Gene Information

Kifc3
Name: kinesin family member C3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16582
HGNC: HGNC:6326
Homologene: 124421
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: Mll3, E330008K23Rik, HALR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231051
Homologene: 46480
Snap25
Name: synaptosomal-associated protein 25
Synonyms: GENA 70, Bdr, SNAP-25, sp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20614
Homologene: 13311
Zeb1
Name: zinc finger E-box binding homeobox 1
Synonyms: [delta]EF1, AREB6, ZEB, Zfhep, 3110032K11Rik, Tcf8, Zfhx1a, Tcf18, Tw, Zfx1a, MEB1, Nil2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 21417
Homologene: 31779
Mdn1
Name: midasin AAA ATPase 1
Synonyms: 4833432B22Rik, LOC213784, D4Abb1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100019
Homologene: 39689
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ankyrin-G, Ank-3, Ankyrin-3, AnkG, 2900054D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Rbm34
Name: RNA binding motif protein 34
Synonyms: 4930547K05Rik, D8Ertd233e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 52202
Homologene: 56691
Tasor2
Name: transcription activation suppressor family member 2
Synonyms: Fam208b, BC016423
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105203
VEGA: 13
Homologene: 26435
Nle1
Name: notchless homolog 1
Synonyms: notchless, l11Jus4, l11Jus1, Nle
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217011
Homologene: 5494
Hectd4
Name: HECT domain E3 ubiquitin protein ligase 4
Synonyms: Gm15800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269700
Homologene: 28297
Shf
Name: Src homology 2 domain containing F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 435684
Homologene: 44524
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77505
Homologene: 131117
Col4a3
Name: collagen, type IV, alpha 3
Synonyms: alpha3(IV), tumstatin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12828
HGNC: HGNC:2204
Homologene: 68033
Cfap46
Name: cilia and flagella associated protein 46
Synonyms: 9330101J02Rik, Ttc40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 212124
H2bl1
Name: H2B.L histone variant 1
Synonyms: 1700024P04Rik, SubH2Bv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 69382
Homologene: 105972
Gm884
Name: predicted gene 884
Synonyms: LOC380730
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380730
Homologene: 134511
Grhpr
Name: glyoxylate reductase/hydroxypyruvate reductase
Synonyms: 6430629L09Rik, 1110059D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 76238
HGNC: HGNC:4570
Homologene: 49088
Olfr799
Name: olfactory receptor 799
Synonyms: GA_x6K02T2PULF-11325750-11326685, MOR114-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258929
Homologene: 37000
Nol4
Name: nucleolar protein 4
Synonyms: LOC383304, 4930568N03Rik, 1700013J13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 319211
HGNC: HGNC:7870
Homologene: 36142
Gli3
Name: GLI-Kruppel family member GLI3
Synonyms: brachyphalangy, Bph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14634
HGNC: HGNC:4319
Homologene: 139
Ceacam20
Name: carcinoembryonic antigen-related cell adhesion molecule 20
Synonyms: 9130012D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71601
Homologene: 19010
Ceacam15
Name: carcinoembryonic antigen-related cell adhesion molecule 15
Synonyms: C430002N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101434
Homologene: 131210
Xkr4
Name: X-linked Kx blood group related 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 497097
Homologene: 45848
Ccdc57
Name: coiled-coil domain containing 57
Synonyms: 4933434G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71276
Homologene: 52351
Nid1
Name: nidogen 1
Synonyms: entactin-1, entactin 1, nidogen-1, entactin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18073
VEGA: 13
HGNC: HGNC:7821
Homologene: 1878
Acoxl
Name: acyl-Coenzyme A oxidase-like
Synonyms: 1200014P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74121
Homologene: 75083
Thrb
Name: thyroid hormone receptor beta
Synonyms: T3R[b], Thrb1, TR beta, c-erbAbeta, Thrb2, T3Rbeta, Nr1a2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21834
Homologene: 36025
CAAA01083517.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Med7
Name: mediator complex subunit 7
Synonyms: Crsp9, Crsp33, 1110063B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66213
HGNC: HGNC:2378
Homologene: 3153
Dnajc9
Name: DnaJ heat shock protein family (Hsp40) member C9
Synonyms: 5330419I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 108671
VEGA: 14
Homologene: 9055
Rps19bp1
Name: ribosomal protein S19 binding protein 1
Synonyms: S19BP, 2510038A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66538
VEGA: 15
Homologene: 18407
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 3,216,813 bp
  • T to A, chromosome 1 at 82,716,365 bp
  • A to G, chromosome 1 at 183,397,837 bp
  • G to A, chromosome 2 at 122,368,682 bp
  • A to T, chromosome 2 at 128,044,366 bp
  • A to T, chromosome 2 at 136,758,348 bp
  • A to T, chromosome 4 at 32,667,089 bp
  • A to G, chromosome 4 at 44,989,045 bp
  • A to T, chromosome 5 at 25,373,484 bp
  • G to A, chromosome 5 at 121,318,624 bp
  • A to G, chromosome 7 at 16,672,024 bp
  • A to G, chromosome 7 at 19,974,192 bp
  • T to A, chromosome 7 at 105,694,153 bp
  • A to T, chromosome 7 at 139,654,629 bp
  • A to G, chromosome 8 at 95,105,772 bp
  • C to T, chromosome 8 at 126,970,893 bp
  • C to T, chromosome 10 at 69,874,835 bp
  • A to T, chromosome 10 at 129,647,544 bp
  • C to G, chromosome 11 at 46,440,633 bp
  • A to G, chromosome 11 at 82,906,419 bp
  • A to G, chromosome 11 at 103,534,641 bp
  • G to T, chromosome 11 at 120,873,802 bp
  • T to C, chromosome 13 at 3,575,049 bp
  • T to C, chromosome 13 at 13,483,790 bp
  • C to T, chromosome 13 at 15,725,996 bp
  • A to T, chromosome 13 at 98,984,515 bp
  • T to A, chromosome 14 at 18,008,642 bp
  • G to A, chromosome 14 at 20,388,697 bp
  • CCTTCTTCTTCTTCTTCTTCTT to CCTTCTTCTTCTTCTTCTT, chromosome 15 at 80,261,049 bp
  • G to T, chromosome 18 at 5,772,699 bp
  • A to T, chromosome 18 at 22,770,681 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1257 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039324-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.