Strain Name:
C57BL/6J-MtgxR1268Btlr/Mmmh
Stock Number:
039335-MU
Citation ID:
RRID:MMRRC_039335-MU
Other Names:
R1268 (G1), C57BL/6J-MtgxR1268Btlr
Major Collection:

Gene Information

Foxp4
Name: forkhead box P4
Synonyms: 1200010K03Rik, 2310007G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74123
Homologene: 12536
Ulk1
Name: unc-51 like kinase 1
Synonyms: Unc51.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22241
Homologene: 2640
Mybl2
Name: myeloblastosis oncogene-like 2
Synonyms: Bmyb, B-Myb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17865
HGNC: HGNC:7548
Homologene: 1847
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105689
Homologene: 9005
Brpf3
Name: bromodomain and PHD finger containing, 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268936
Homologene: 16092
Plk4
Name: polo like kinase 4
Synonyms: Sak, Stk18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20873
Homologene: 7962
Rbm27
Name: RNA binding motif protein 27
Synonyms: Psc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225432
VEGA: 18
Homologene: 35410
Arap2
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
Synonyms: Centd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 212285
Homologene: 9064
Afdn
Name: afadin, adherens junction formation factor
Synonyms: AF6, Afadin, S-afadin, I-afadin, 5033403D15Rik, Mllt4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17356
HGNC: HGNC:7137
Homologene: 21202
Tenm2
Name: teneurin transmembrane protein 2
Synonyms: Ten-m2, D3Bwg1534e, 9330187F13Rik, 2610040L17Rik, Odz2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 23964
Homologene: 22672
Nek1
Name: NIMA (never in mitosis gene a)-related expressed kinase 1
Synonyms: kat, kidney, anemia and testis, D8Ertd790e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18004
HGNC: HGNC:7744
Homologene: 14376
Gnat1
Name: guanine nucleotide binding protein, alpha transducing 1
Synonyms: transducin, Tralpha, Gnat-1, Ird2, Ird1, irdr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 14685
HGNC: HGNC:4393
Homologene: 20084
Crebrf
Name: CREB3 regulatory factor
Synonyms: A930001N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77128
Homologene: 12672
Ntn1
Name: netrin 1
Synonyms: Netrin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18208
HGNC: HGNC:8029
Homologene: 21008
Hs6st1
Name: heparan sulfate 6-O-sulfotransferase 1
Synonyms: 6OST1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 50785
HGNC: HGNC:5201
Homologene: 75051
Ints2
Name: integrator complex subunit 2
Synonyms: 2810417D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70422
Homologene: 10801
Nupl1
Name: nucleoporin like 1
Synonyms: 1700017F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71844
VEGA: 14
Homologene: 40924
Shf
Name: Src homology 2 domain containing F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 435684
Homologene: 44524
Aplnr
Name: apelin receptor
Synonyms: msr/apj, apelin receptor, APJ, Agtrl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 23796
HGNC: HGNC:339
Homologene: 3784
Myh7b
Name: myosin, heavy chain 7B, cardiac muscle, beta
Synonyms: Myh14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668940
Homologene: 66117
Ulk4
Name: unc-51-like kinase 4
Synonyms: 4932415A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 209012
Homologene: 41205
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, OTTMUSG00000020804, Heatr7b1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Olfr1308
Name: olfactory receptor 1308
Synonyms: GA_x6K02T2Q125-73008844-73007882, MOR245-22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258258
Homologene: 45797
Samd9l
Name: sterile alpha motif domain containing 9-like
Synonyms: ESTM25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 209086
HGNC: HGNC:1349
Homologene: 7707
Olfr494
Name: olfactory receptor 494
Synonyms: GA_x6K02T2PBJ9-10697517-10698461, MOR204-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258732
Homologene: 133602
Col5a1
Name: collagen, type V, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12831
HGNC: HGNC:2209
Homologene: 55434
Igsf11
Name: immunoglobulin superfamily, member 11
Synonyms: BT-IgSF, 1700025L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 207683
Homologene: 17613
Notum
Name: notum palmitoleoyl-protein carboxylesterase
Synonyms: 5730593N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 77583
Homologene: 45335
Olfr1451
Name: olfactory receptor 1451
Synonyms: GA_x6K02T2RE5P-3328502-3329434, MOR202-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258700
Homologene: 128082
Vdr
Name: vitamin D (1,25-dihydroxyvitamin D3) receptor
Synonyms: Nr1i1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22337
Homologene: 37297
Slc35f2
Name: solute carrier family 35, member F2
Synonyms: 1500009K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72022
VEGA: 9
Homologene: 36363
Olfr834
Name: olfactory receptor 834
Synonyms: GA_x6K02T2PVTD-12724921-12725859, MOR153-2, MOR153-4_p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258074
HGNC: HGNC:8467
Homologene: 128143
Olfr48
Name: olfactory receptor 48
Synonyms: IC3, MOR232-5, GA_x6K02T2Q125-51285881-51284976
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18347
Homologene: 85936
Olfr1364
Name: olfactory receptor 1364
Synonyms: GA_x6K02T2QHY8-11663090-11664010, MOR256-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 258533
Homologene: 64885
Fmo6
Name: flavin containing monooxygenase 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226565
Homologene: 68130
Mab21l3
Name: mab-21-like 3
Synonyms: BC037703
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242125
Homologene: 17564
Rnaseh2b
Name: ribonuclease H2, subunit B
Synonyms: 2610207P08Rik, Dleu8, 1110019N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67153
Homologene: 41572
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 36,068,926 bp
  • C to T, chromosome 1 at 88,230,680 bp
  • A to T, chromosome 1 at 162,920,517 bp
  • A to T, chromosome 2 at 28,002,489 bp
  • A to G, chromosome 2 at 85,137,431 bp
  • A to G, chromosome 2 at 89,844,154 bp
  • G to T, chromosome 2 at 111,960,877 bp
  • G to A, chromosome 2 at 122,368,682 bp
  • C to A, chromosome 2 at 155,614,046 bp
  • A to G, chromosome 2 at 163,074,716 bp
  • T to A, chromosome 3 at 40,811,369 bp
  • C to T, chromosome 3 at 101,835,047 bp
  • A to G, chromosome 5 at 62,730,621 bp
  • A to G, chromosome 5 at 110,790,277 bp
  • C to T, chromosome 6 at 3,376,113 bp
  • A to T, chromosome 7 at 108,367,795 bp
  • C to A, chromosome 8 at 61,022,264 bp
  • T to C, chromosome 9 at 18,988,356 bp
  • T to A, chromosome 9 at 53,797,913 bp
  • A to G, chromosome 9 at 107,675,877 bp
  • C to A, chromosome 9 at 121,257,074 bp
  • C to T, chromosome 11 at 36,063,177 bp
  • TCCTCGGC to TC, chromosome 11 at 68,213,133 bp
  • C to T, chromosome 11 at 86,233,085 bp
  • C to T, chromosome 11 at 120,658,667 bp
  • C to T, chromosome 13 at 21,574,328 bp
  • A to T, chromosome 14 at 60,244,670 bp
  • A to T, chromosome 14 at 62,372,455 bp
  • G to A, chromosome 14 at 103,208,782 bp
  • T to C, chromosome 15 at 97,857,475 bp
  • A to G, chromosome 16 at 39,024,854 bp
  • T to C, chromosome 17 at 13,887,986 bp
  • CTTTT to CTTT, chromosome 17 at 26,739,596 bp
  • A to G, chromosome 17 at 28,836,556 bp
  • G to C, chromosome 17 at 47,880,353 bp
  • T to C, chromosome 18 at 42,333,302 bp
  • T to C, chromosome 19 at 12,999,261 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1268 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039335-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.