Strain Name:
C57BL/6J-MtgxR1272Btlr/Mmmh
Stock Number:
039338-MU
Citation ID:
RRID:MMRRC_039338-MU
Other Names:
R1272 (G1), C57BL/6J-MtgxR1272Btlr
Major Collection:

Gene Information

Chrna3
Name: cholinergic receptor, nicotinic, alpha polypeptide 3
Synonyms: Acra3, Acra-3, A730007P14Rik, neuronal nicotinic acetylcholine receptor, alpha 3 subunit, alpha 3, (a)3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 110834
VEGA: 9
HGNC: HGNC:1957
Homologene: 591
Bzw1
Name: basic leucine zipper and W2 domains 1
Synonyms: 1200015E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 66882
Homologene: 41651
Dop1a
Name: DOP1 leucine zipper like protein A
Synonyms: B130005I07Rik, Dopey1, D9Ertd809e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 320615
Homologene: 26645
Leo1
Name: Leo1, Paf1/RNA polymerase II complex component
Synonyms: LOC235497
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235497
VEGA: 9
Homologene: 133895
Tfcp2l1
Name: transcription factor CP2-like 1
Synonyms: Crtr-1, D930018N21Rik, Cp2l1, Tcfcp2l1, 4932442M07Rik, LBP-9, 1810030F05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 81879
Homologene: 8730
Rnf20
Name: ring finger protein 20
Synonyms: 4833430L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 109331
Homologene: 5571
Mki67
Name: antigen identified by monoclonal antibody Ki 67
Synonyms: D630048A14Rik, Ki67, Ki-67
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 17345
HGNC: HGNC:7107
Homologene: 1814
Tenm2
Name: teneurin transmembrane protein 2
Synonyms: D3Bwg1534e, 2610040L17Rik, Ten-m2, Odz2, 9330187F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 23964
Homologene: 22672
Cd320
Name: CD320 antigen
Synonyms: NG29, VLDL, 425O18-1, 8D6, D17Ertd716e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 54219
Homologene: 9573
Nupl1
Name: nucleoporin like 1
Synonyms: 1700017F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 71844
VEGA: 14
Homologene: 40924
Usp54
Name: ubiquitin specific peptidase 54
Synonyms: 4930429G18Rik, C030002J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 78787
Homologene: 90902
Kel
Name: Kell blood group
Synonyms: CD238
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 23925
HGNC: HGNC:6308
Homologene: 362
Aldh3b1
Name: aldehyde dehydrogenase 3 family, member B1
Synonyms: 1700001N19Rik, ALDH7, ALDH4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 67689
VEGA: 19
HGNC: HGNC:410
Homologene: 73890
Rdh10
Name: retinol dehydrogenase 10 (all-trans)
Synonyms: 4921506A21Rik, m366Asp, 3110069K09Rik, D1Ertd762e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 98711
Homologene: 14061
Cmya5
Name: cardiomyopathy associated 5
Synonyms: 2310076E16Rik, Myospryn, 2310076E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 76469
VEGA: 13
Homologene: 137367
Nav3
Name: neuron navigator 3
Synonyms: 9630020C08Rik, Pomfil1p, 4732483H20Rik, unc53H3, steerin 3, POMFIL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 260315
Homologene: 56688
Ltbp1
Name: latent transforming growth factor beta binding protein 1
Synonyms: 9430031G15Rik, b2b1000Clo, LTBP-1, Ltbp1L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 268977
HGNC: HGNC:6714
Homologene: 522
Per2
Name: period circadian clock 2
Synonyms: mPer2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 18627
HGNC: HGNC:8846
Homologene: 7885
Ksr1
Name: kinase suppressor of ras 1
Synonyms: D11Bhm183e, B-KSR1, D11Bhm184e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 16706
HGNC: HGNC:6465
Homologene: 8410
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: Cav2.3, Cchra1, alpha1E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Ap1m2
Name: adaptor protein complex AP-1, mu 2 subunit
Synonyms: D9Ertd818e, [m]1B, mu1B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 11768
VEGA: 9
HGNC: HGNC:558
Homologene: 55906
Zfp616
Name: zinc finger protein 616
Synonyms: Gm12330
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 327963
Homologene: 88945
Ric8a
Name: RIC8 guanine nucleotide exchange factor A
Synonyms: synembryn, RIC-8, Ric8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 101489
Homologene: 23331
4921509C19Rik
Name: RIKEN cDNA 4921509C19 gene
Synonyms: LOC381389
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 381393
Homologene: 72396
Mapre3
Name: microtubule-associated protein, RP/EB family, member 3
Synonyms: EB3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100732
HGNC: HGNC:6892
Homologene: 56565
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 16,108,016 bp
  • T to C, chromosome 1 at 58,397,820 bp
  • A to G, chromosome 1 at 91,440,826 bp
  • C to A, chromosome 1 at 118,632,313 bp
  • T to C, chromosome 1 at 154,444,968 bp
  • A to G, chromosome 2 at 151,472,057 bp
  • A to G, chromosome 4 at 49,651,496 bp
  • T to A, chromosome 5 at 30,861,888 bp
  • A to T, chromosome 6 at 41,703,470 bp
  • T to A, chromosome 7 at 135,700,414 bp
  • T to C, chromosome 7 at 140,862,376 bp
  • A to T, chromosome 9 at 21,305,710 bp
  • A to G, chromosome 9 at 55,024,282 bp
  • G to A, chromosome 9 at 75,450,513 bp
  • A to G, chromosome 9 at 86,521,424 bp
  • G to C, chromosome 10 at 109,736,999 bp
  • C to T, chromosome 11 at 36,063,177 bp
  • A to T, chromosome 11 at 74,085,236 bp
  • G to A, chromosome 11 at 79,146,078 bp
  • T to C, chromosome 13 at 93,095,112 bp
  • C to T, chromosome 14 at 20,561,110 bp
  • A to T, chromosome 14 at 60,244,670 bp
  • T to C, chromosome 17 at 33,848,164 bp
  • A to G, chromosome 17 at 75,225,285 bp
  • A to G, chromosome 19 at 3,921,746 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1272 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039338-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.