Strain Name:
C57BL/6J-MtgxR1274Btlr/Mmmh
Stock Number:
039340-MU
Citation ID:
RRID:MMRRC_039340-MU
Other Names:
R1274 (G1), C57BL/6J-MtgxR1274Btlr
Major Collection:

Gene Information

Snx30
Name: sorting nexin family member 30
Synonyms: C030041J06Rik, 4732481H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 209131
Homologene: 2811
Nav2
Name: neuron navigator 2
Synonyms: POMFIL2, Unc53H2, RAINB2, 5330421F07Rik, HELAD1, Rainb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 78286
Homologene: 52330
Dync1h1
Name: dynein cytoplasmic 1 heavy chain 1
Synonyms: dynein heavy chain, retrograde transport, Dnchc1, Loa, MAP1C, Swl, Dnec1, 9930018I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 13424
HGNC: HGNC:2961
Homologene: 1053
Zfp335
Name: zinc finger protein 335
Synonyms: 1810045J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 329559
Homologene: 11129
Gm6563
Name: predicted pseudogene 6563
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 625193
VEGA: 19
Aak1
Name: AP2 associated kinase 1
Synonyms: D6Ertd245e, 5530400K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 269774
Homologene: 128746
Agap3
Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 3
Synonyms: Centg3, Crag, MRIP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 213990
Homologene: 23742
Slc7a5
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
Synonyms: D0H16S474E, TA1, LAT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 20539
Homologene: 55759
Anpep
Name: alanyl (membrane) aminopeptidase
Synonyms: Apn, Cd13, aminopeptidase N
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 16790
HGNC: HGNC:500
Homologene: 68163
Ptpn13
Name: protein tyrosine phosphatase, non-receptor type 13
Synonyms: Ptpri, PTPL1, PTP-BL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 19249
HGNC: HGNC:9646
Homologene: 7909
Sik1
Name: salt inducible kinase 1
Synonyms: Msk, Snf1lk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 17691
VEGA: 17
Homologene: 7847
Olfr1335
Name: olfactory receptor 1335
Synonyms: Olfr1335, MOR259-5, MOR259-12, GA_x6K02T2QD9B-18726774-18727577, Olfr1334-ps1, GA_x6K02T2QD9B-18723799-18724749
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 435804
Homologene: 138635
Sorbs2
Name: sorbin and SH3 domain containing 2
Synonyms: 2010203O03Rik, 9430041O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234214
Homologene: 83295
Afap1l2
Name: actin filament associated protein 1-like 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 226250
Homologene: 13057
Col16a1
Name: collagen, type XVI, alpha 1
Synonyms: A530052M23Rik, 2700007F12Rik, [a]1 (XVI) collagen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 107581
HGNC: HGNC:2193
Homologene: 1397
Ceacam3
Name: carcinoembryonic antigen-related cell adhesion molecule 3
Synonyms: EG384557, cea12, Psg24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 384557
Homologene: 86971
Rgs20
Name: regulator of G-protein signaling 20
Synonyms: 2900073E09Rik, Rgsz1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 58175
Homologene: 2745
Vmn1r234
Name: vomeronasal 1 receptor 234
Synonyms: V1rf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 171232
P2ry12
Name: purinergic receptor P2Y, G-protein coupled 12
Synonyms: 4921504D23Rik, 2900079B22Rik, P2Y12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 70839
Homologene: 11260
Cyp4a14
Name: cytochrome P450, family 4, subfamily a, polypeptide 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 13119
Homologene: 134697
Gm11487
Name: predicted gene 11487
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 433719
Homologene: 128423
Olfr1163
Name: olfactory receptor 1163
Synonyms: MOR174-8, GA_x6K02T2Q125-49563265-49562315
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258638
Homologene: 17328
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 4,912,447 bp
  • A to C, chromosome 2 at 88,070,595 bp
  • GTCCTCCTCCTCCTCCTC to GTCCTCCTCCTCCTC, chromosome 2 at 164,907,468 bp
  • T to C, chromosome 3 at 59,217,220 bp
  • A to G, chromosome 4 at 59,885,133 bp
  • T to C, chromosome 4 at 73,403,076 bp
  • T to C, chromosome 4 at 115,489,953 bp
  • T to C, chromosome 4 at 118,809,396 bp
  • T to A, chromosome 4 at 130,097,801 bp
  • T to A, chromosome 5 at 24,476,451 bp
  • C to T, chromosome 5 at 103,550,260 bp
  • T to C, chromosome 6 at 86,949,528 bp
  • A to G, chromosome 7 at 17,163,139 bp
  • C to A, chromosome 7 at 49,604,430 bp
  • C to T, chromosome 7 at 79,838,256 bp
  • T to C, chromosome 8 at 45,741,588 bp
  • C to T, chromosome 8 at 121,883,714 bp
  • G to A, chromosome 12 at 110,636,509 bp
  • CTT to CTTT, chromosome 17 at 21,229,251 bp
  • A to T, chromosome 17 at 31,846,575 bp
  • A to T, chromosome 19 at 23,676,337 bp
  • T to C, chromosome 19 at 56,914,563 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1274 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039340-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.