Strain Name:
C57BL/6J-MtgxR1277Btlr/Mmmh
Stock Number:
039343-MU
Citation ID:
RRID:MMRRC_039343-MU
Other Names:
R1277 (G1), C57BL/6J-MtgxR1277Btlr
Major Collection:

Gene Information

Abca12
Name: ATP-binding cassette, sub-family A (ABC1), member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74591
Homologene: 45441
Uhrf1bp1
Name: UHRF1 (ICBP90) binding protein 1
Synonyms: F830021D11Rik, 1110020K19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224648
Homologene: 9816
Itpr1
Name: inositol 1,4,5-trisphosphate receptor 1
Synonyms: IP3R1, opt, Pcp1, wblo, Pcp-1, Ip3r, P400, InsP3R type I, Itpr-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 16438
HGNC: HGNC:6180
Homologene: 1673
Crlf2
Name: cytokine receptor-like factor 2
Synonyms: Tslpr, Tpte2, Ly114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 57914
Homologene: 49476
Akap8l
Name: A kinase (PRKA) anchor protein 8-like
Synonyms: Nakap95
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 54194
VEGA: 17
Homologene: 8658
Slc17a8
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8
Synonyms: Vglut3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 216227
Homologene: 13584
Pkd2
Name: polycystin 2, transient receptor potential cation channel
Synonyms: polycystin-2, TRPP2, PC2, C030034P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 18764
HGNC: HGNC:9009
Homologene: 20104
Chd6
Name: chromodomain helicase DNA binding protein 6
Synonyms: 5430439G14Rik, 6330406J24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 71389
Homologene: 32772
Appl1
Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Synonyms: 2900057D21Rik, 7330406P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 72993
Homologene: 32143
Rfx7
Name: regulatory factor X, 7
Synonyms: D130086K05Rik, 2510005N23Rik, Rfxdc2, 9930116O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 319758
Homologene: 11275
Clk4
Name: CDC like kinase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 12750
Homologene: 56895
Pcdhb11
Name: protocadherin beta 11
Synonyms: Pcdhb5E, PcdhbK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93882
Homologene: 62178
Ralgps1
Name: Ral GEF with PH domain and SH3 binding motif 1
Synonyms: RALGEF2, 5830418G11Rik, RALGPS1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241308
Homologene: 65163
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Nudt12
Name: nudix (nucleoside diphosphate linked moiety X)-type motif 12
Synonyms: 0610016O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 67993
Homologene: 44188
Prl3c1
Name: prolactin family 3, subfamily c, member 1
Synonyms: Prlpj, PLP I, PLP-J
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 27372
Homologene: 49353
Vamp8
Name: vesicle-associated membrane protein 8
Synonyms: endobrevin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 22320
Homologene: 37846
Zfp414
Name: zinc finger protein 414
Synonyms: 0610030H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 328801
Homologene: 12264
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 71,288,526 bp
  • A to G, chromosome 2 at 33,174,425 bp
  • A to G, chromosome 2 at 40,725,945 bp
  • T to A, chromosome 2 at 52,273,878 bp
  • A to T, chromosome 2 at 160,967,815 bp
  • A to G, chromosome 5 at 104,502,359 bp
  • C to T, chromosome 5 at 109,557,600 bp
  • A to G, chromosome 6 at 72,385,668 bp
  • A to G, chromosome 6 at 108,339,621 bp
  • A to G, chromosome 9 at 72,593,312 bp
  • T to C, chromosome 10 at 89,597,457 bp
  • A to G, chromosome 11 at 51,267,189 bp
  • T to C, chromosome 13 at 27,203,572 bp
  • A to C, chromosome 14 at 26,927,856 bp
  • T to C, chromosome 17 at 27,890,071 bp
  • C to T, chromosome 17 at 32,332,483 bp
  • G to T, chromosome 17 at 33,630,475 bp
  • G to A, chromosome 17 at 59,010,136 bp
  • A to T, chromosome 18 at 37,421,716 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1277 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039343-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.