Strain Name:
C57BL/6J-MtgxR1309Btlr/Mmmh
Stock Number:
039375-MU
Citation ID:
RRID:MMRRC_039375-MU
Other Names:
R1309 (G1), C57BL/6J-MtgxR1309Btlr
Major Collection:

Strain Information

Kera
Name: keratocan
Synonyms: SLRR2B, CNA2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16545
VEGA: 10
HGNC: HGNC:6309
Homologene: 5106
Stk24
Name: serine/threonine kinase 24
Synonyms: STE20, 1810013H02Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 223255
VEGA: 14
Homologene: 20793
Prep
Name: prolyl endopeptidase
Synonyms: Pop, prolyl oligopeptidase, D10Wsu136e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19072
VEGA: 10
HGNC: HGNC:9358
Homologene: 2042
Lmnb1
Name: lamin B1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16906
HGNC: HGNC:6637
Homologene: 55912
Cdkal1
Name: CDK5 regulatory subunit associated protein 1-like 1
Synonyms: 6620401C13Rik, 1190005B03Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68916
Homologene: 9830
Cpsf7
Name: cleavage and polyadenylation specific factor 7
Synonyms: C330017N18Rik, 5730453I16Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 269061
VEGA: 19
Homologene: 11731
Slit2
Name: slit guidance ligand 2
Synonyms: E130320P19Rik, Slil3, Drad-1, b2b1200.1Clo, E030015M03Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20563
Homologene: 3516
Angptl2
Name: angiopoietin-like 2
Synonyms: Arp2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 26360
HGNC: HGNC:490
Homologene: 22695
Dhx37
Name: DEAH-box helicase 37
Synonyms: LOC381671, LOC208144
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 208144
Homologene: 6641
Tdrd6
Name: tudor domain containing 6
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210510
Homologene: 19364
Ankar
Name: ankyrin and armadillo repeat containing
Synonyms: 4932422E22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 319695
Homologene: 85163
Gstm7
Name: glutathione S-transferase, mu 7
Synonyms: 0610005A07Rik, Cd203c
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68312
HGNC: HGNC:4634
Homologene: 41816
Ccm2l
Name: cerebral cavernous malformation 2-like
Synonyms: BC020535
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228788
Homologene: 130991
Rxfp1
Name: relaxin/insulin-like family peptide receptor 1
Synonyms: LOC381489, Lgr7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 381489
Homologene: 11007
Or2ad1
Name: olfactory receptor family 2 subfamily AD member 1
Synonyms: Olfr1368, MOR256-15, GA_x6K02T2QHY8-12104556-12105500
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 258527
Homologene: 105156
Snx14
Name: sorting nexin 14
Synonyms: YR-14, C330035N22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244962
Homologene: 17823
Asb2
Name: ankyrin repeat and SOCS box-containing 2
Synonyms: 1110008E15Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 65256
Homologene: 69202
Ac155333.3
Name: Ig kappa chain V-V region K2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Ccdc154
Name: coiled-coil domain containing 154
Synonyms: ntl, LOC207209
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 207209
Homologene: 19361
Spag6l
Name: sperm associated antigen 6-like
Synonyms: PF16, Spag6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 50525
Homologene: 8252
Alkbh2
Name: alkB homolog 2, alpha-ketoglutarate-dependent dioxygenase
Synonyms: Abh2, mABH2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231642
Homologene: 18393
Dip2a
Name: disco interacting protein 2 homolog A
Synonyms: Dip2, 4931420H10Rik, Kiaa0184-hp
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64451
Homologene: 41012
Zswim2
Name: zinc finger SWIM-type containing 2
Synonyms: 4933437F18Rik, 1700025P14Rik, MEX
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71861
Homologene: 32689
Tedc1
Name: tubulin epsilon and delta complex 1
Synonyms: 4930427A07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104732
Homologene: 77129
AC158673.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Spag6
Name: sperm associated antigen 6
Synonyms: BC061194, Spag6l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 381350
Homologene: 133723
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 72,674,004 bp
  • A to G, chromosome 2 at 18,734,216 bp
  • G to A, chromosome 2 at 33,246,128 bp
  • A to T, chromosome 2 at 83,938,756 bp
  • A to T, chromosome 2 at 153,070,924 bp
  • C to T, chromosome 3 at 79,663,292 bp
  • G to A, chromosome 3 at 107,930,379 bp
  • T to C, chromosome 5 at 48,296,398 bp
  • C to T, chromosome 5 at 114,124,226 bp
  • C to T, chromosome 5 at 125,417,438 bp
  • G to T, chromosome 6 at 69,607,741 bp
  • A to G, chromosome 6 at 69,858,720 bp
  • A to C, chromosome 9 at 88,403,435 bp
  • A to G, chromosome 10 at 45,126,026 bp
  • A to G, chromosome 10 at 76,279,776 bp
  • A to G, chromosome 10 at 97,609,426 bp
  • A to G, chromosome 12 at 103,325,408 bp
  • A to G, chromosome 12 at 113,161,780 bp
  • C to T, chromosome 13 at 21,142,167 bp
  • T to A, chromosome 13 at 29,357,583 bp
  • A to G, chromosome 14 at 121,302,786 bp
  • T to C, chromosome 17 at 25,170,991 bp
  • T to C, chromosome 17 at 43,626,621 bp
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 18 at 56,739,904 bp
  • T to C, chromosome 19 at 10,533,467 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1309 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039375-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.