Strain Name:
C57BL/6J-MtgxR1322Btlr/Mmmh
Stock Number:
039388-MU
Citation ID:
RRID:MMRRC_039388-MU
Other Names:
R1322 (G1), C57BL/6J-MtgxR1322Btlr
Major Collection:

Gene Information

Zfp930
Name: zinc finger protein 930
Synonyms: D10627, zinc finger protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234358
Aco2
Name: aconitase 2, mitochondrial
Synonyms: Aco3, Aco-2, D10Wsu183e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 11429
HGNC: HGNC:118
Homologene: 856
Polr1c
Name: polymerase (RNA) I polypeptide C
Synonyms: 40kDa, RPA40, Rpo1-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 20016
Homologene: 3586
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: trithorax Drosophila, HTRX1, Cxxc7, Mll1, All1, Mll, ALL-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Sppl3
Name: signal peptide peptidase 3
Synonyms: 4833416I09Rik, Usmg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 74585
Homologene: 15563
Prpf39
Name: pre-mRNA processing factor 39
Synonyms: Srcs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 328110
Homologene: 32377
Tnc
Name: tenascin C
Synonyms: Hxb, TN, tenascin-C, cytotactin, C130033P17Rik, hexabrachion, TN-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 21923
HGNC: HGNC:5318
Homologene: 55636
Nrbp1
Name: nuclear receptor binding protein 1
Synonyms: Nrbp, B230344L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 192292
HGNC: HGNC:7993
Homologene: 8373
Mecom
Name: MDS1 and EVI1 complex locus
Synonyms: Evi1, MDS1-EVI1, Jbo, Prdm3, D630039M04Rik, Evi-1, Mds1, ZNFPR1B1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 14013
HGNC: HGNC:3498
Homologene: 21086
Col27a1
Name: collagen, type XXVII, alpha 1
Synonyms: 5730512J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 373864
Homologene: 69400
Zfp251
Name: zinc finger protein 251
Synonyms: 9130001M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 71591
VEGA: 15
Homologene: 87789
Hipk1
Name: homeodomain interacting protein kinase 1
Synonyms: 1110062K04Rik, Myak
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 15257
Homologene: 56483
Vmn2r88
Name: vomeronasal 2, receptor 88
Synonyms: V2r13, V2r3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 669149
Homologene: 129606
Kmt2b
Name: lysine (K)-specific methyltransferase 2B
Synonyms: 2610014H22Rik, Mll2, Wbp7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 75410
Homologene: 22838
Gli3
Name: GLI-Kruppel family member GLI3
Synonyms: brachyphalangy, Bph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 14634
HGNC: HGNC:4319
Homologene: 139
Gjb3
Name: gap junction protein, beta 3
Synonyms: Gjb-3, connexin 31, Cx31, Cnx31, D4Wsu144e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 14620
HGNC: HGNC:4285
Homologene: 7338
Itih2
Name: inter-alpha trypsin inhibitor, heavy chain 2
Synonyms: Itih-2, Intin2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16425
HGNC: HGNC:6167
Homologene: 1668
Olfr830
Name: olfactory receptor 830
Synonyms: GA_x6K02T2PVTD-12618399-12619337, MOR152-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258559
Homologene: 79360
Fam184a
Name: family with sequence similarity 184, member A
Synonyms: 3110012E06Rik, 4930589M24Rik, 4930438C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 75906
Homologene: 11600
Trgc3
Name: T cell receptor gamma, constant 3
Synonyms: Gm17356, Tcrg-C3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 107575
Mgat4d
Name: MGAT4 family, member C
Synonyms: 4933434I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67555
Homologene: 128538
Sh3bp2
Name: SH3-domain binding protein 2
Synonyms: 3BP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 24055
Homologene: 2276
Gpr162
Name: G protein-coupled receptor 162
Synonyms: Grca, A-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 14788
Homologene: 8400
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 10,109,522 bp
  • G to T, chromosome 3 at 29,957,373 bp
  • A to T, chromosome 3 at 103,743,981 bp
  • A to C, chromosome 4 at 63,328,566 bp
  • C to T, chromosome 4 at 64,013,994 bp
  • G to A, chromosome 4 at 127,326,431 bp
  • T to A, chromosome 5 at 31,245,813 bp
  • T to A, chromosome 5 at 34,555,493 bp
  • TGG to TG, chromosome 5 at 115,088,293 bp
  • G to T, chromosome 6 at 124,858,901 bp
  • G to A, chromosome 7 at 30,578,475 bp
  • A to G, chromosome 8 at 69,228,168 bp
  • A to G, chromosome 8 at 83,365,725 bp
  • T to C, chromosome 9 at 18,875,521 bp
  • A to G, chromosome 9 at 44,821,121 bp
  • G to T, chromosome 10 at 53,652,319 bp
  • A to T, chromosome 12 at 65,042,662 bp
  • C to T, chromosome 13 at 15,714,936 bp
  • T to C, chromosome 13 at 19,261,194 bp
  • T to G, chromosome 14 at 51,414,108 bp
  • C to T, chromosome 15 at 76,854,236 bp
  • C to T, chromosome 15 at 81,895,193 bp
  • T to C, chromosome 17 at 46,244,163 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1322 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039388-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.