Strain Name:
C57BL/6J-MtgxR1387Btlr/Mmmh
Stock Number:
039449-MU
Citation ID:
RRID:MMRRC_039449-MU
Other Names:
R1387 (G1), C57BL/6J-MtgxR1387Btlr
Major Collection:

Gene Information

Gene Symbol: Mecp2 [MGI:99918] (Mus musculus (mouse))
Name: methyl CpG binding protein 2
Synonyms: WBP10, D630021H01Rik, 1500041B07Rik, Mbd5
Chromosome: X
Alteration at locus: Chemically Induced
Gene Symbol: Foxq1 [MGI:1298228] (Mus musculus (mouse))
Name: forkhead box Q1
Synonyms: HFH-1, Hfh1, Hfh1l, sa
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Cntnap2 [MGI:1914047] (Mus musculus (mouse))
Name: contactin associated protein-like 2
Synonyms: Caspr2, 5430425M22Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Eno1 [MGI:95393] (Mus musculus (mouse))
Name: enolase 1, alpha non-neuron
Synonyms: 2-phospho-D-glycerate hydrolase, alpha-enolase, c-Myc promoter binding protein, MBP-1, Eno-1
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Nos1 [MGI:97360] (Mus musculus (mouse))
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, 2310005C01Rik, Nos-1, NO
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Nmu [MGI:1860476] (Mus musculus (mouse))
Name: neuromedin U
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Map1b [MGI:1306778] (Mus musculus (mouse))
Name: microtubule-associated protein 1B
Synonyms: Mtap-5, LC1, Mtap1b, MAP5, Mtap5
Chromosome: 13
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 20,555,223 bp
  • A to G, chromosome 1 at 66,606,661 bp
  • A to G, chromosome 1 at 90,822,416 bp
  • A to T, chromosome 1 at 93,055,950 bp
  • T to G, chromosome 1 at 121,491,189 bp
  • C to A, chromosome 1 at 130,804,642 bp
  • G to T, chromosome 1 at 166,400,138 bp
  • T to C, chromosome 2 at 25,677,137 bp
  • T to C, chromosome 2 at 32,565,623 bp
  • T to G, chromosome 2 at 37,104,868 bp
  • C to A, chromosome 2 at 69,456,918 bp
  • GA to GAA, chromosome 2 at 90,917,709 bp
  • T to C, chromosome 2 at 112,244,676 bp
  • T to A, chromosome 2 at 119,070,730 bp
  • T to A, chromosome 2 at 119,705,645 bp
  • T to A, chromosome 2 at 122,303,987 bp
  • A to G, chromosome 3 at 58,691,514 bp
  • T to C, chromosome 4 at 86,374,993 bp
  • T to A, chromosome 4 at 96,634,285 bp
  • C to T, chromosome 4 at 150,248,133 bp
  • G to T, chromosome 4 at 155,899,480 bp
  • G to C, chromosome 5 at 45,450,138 bp
  • A to T, chromosome 5 at 76,350,145 bp
  • T to C, chromosome 5 at 114,200,512 bp
  • A to G, chromosome 5 at 117,206,655 bp
  • T to C, chromosome 5 at 117,953,783 bp
  • G to T, chromosome 5 at 122,880,268 bp
  • A to G, chromosome 5 at 146,211,245 bp
  • A to C, chromosome 6 at 42,624,578 bp
  • T to A, chromosome 6 at 43,307,198 bp
  • T to C, chromosome 6 at 47,107,914 bp
  • C to T, chromosome 6 at 142,461,283 bp
  • T to C, chromosome 7 at 3,415,156 bp
  • T to C, chromosome 7 at 29,206,864 bp
  • A to G, chromosome 7 at 42,046,097 bp
  • A to G, chromosome 7 at 44,732,941 bp
  • T to C, chromosome 7 at 75,586,193 bp
  • T to A, chromosome 7 at 85,558,761 bp
  • T to A, chromosome 7 at 102,484,704 bp
  • A to G, chromosome 7 at 123,466,668 bp
  • T to C, chromosome 8 at 13,792,118 bp
  • T to C, chromosome 9 at 7,125,816 bp
  • T to A, chromosome 9 at 7,282,033 bp
  • C to T, chromosome 9 at 79,681,375 bp
  • T to A, chromosome 9 at 98,186,759 bp
  • T to A, chromosome 9 at 105,644,291 bp
  • T to A, chromosome 9 at 114,420,363 bp
  • T to A, chromosome 11 at 5,104,121 bp
  • C to T, chromosome 11 at 9,682,085 bp
  • A to G, chromosome 11 at 34,273,309 bp
  • T to A, chromosome 11 at 48,895,015 bp
  • G to T, chromosome 11 at 115,853,132 bp
  • A to G, chromosome 11 at 118,182,346 bp
  • G to T, chromosome 12 at 4,274,790 bp
  • C to A, chromosome 12 at 72,915,434 bp
  • C to T, chromosome 12 at 73,942,292 bp
  • C to A, chromosome 12 at 84,152,931 bp
  • C to T, chromosome 12 at 103,628,241 bp
  • G to A, chromosome 13 at 23,137,308 bp
  • A to G, chromosome 13 at 31,559,305 bp
  • A to T, chromosome 13 at 48,586,320 bp
  • A to G, chromosome 13 at 81,493,176 bp
  • T to C, chromosome 13 at 99,432,650 bp
  • T to C, chromosome 14 at 59,860,270 bp
  • A to G, chromosome 14 at 122,459,485 bp
  • C to A, chromosome 15 at 9,106,782 bp
  • T to C, chromosome 15 at 10,380,610 bp
  • C to T, chromosome 15 at 78,298,214 bp
  • T to C, chromosome 16 at 19,606,027 bp
  • T to A, chromosome 16 at 33,900,515 bp
  • C to T, chromosome 16 at 45,825,994 bp
  • A to G, chromosome 17 at 33,816,231 bp
  • T to A, chromosome 17 at 75,538,269 bp
  • A to G, chromosome 18 at 31,983,752 bp
  • A to G, chromosome 18 at 36,196,739 bp
  • A to G, chromosome 18 at 62,986,212 bp
  • G to T, chromosome 18 at 74,644,201 bp
  • A to G, chromosome 19 at 29,723,453 bp
  • G to T, chromosome 19 at 32,798,096 bp
  • C to T, chromosome X at 18,253,996 bp
  • G to A, chromosome X at 74,035,788 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1387 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039449-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.