Beutler Mutagenetix

Strain Name:
C57BL/6J-MtgxR1393Btlr/Mmmh
Stock Number:
039455-MU
Citation ID:
RRID:MMRRC_039455-MU
Other Names:
R1393 (G1), C57BL/6J-MtgxR1393Btlr
Major Collection:

Gene Information

Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: Glur2, Glur-2, GluR2, GluA2, GluR-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Cwf19l2
Name: CWF19-like 2, cell cycle control (S. pombe)
Synonyms: 3230401L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 244672
Homologene: 12366
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12695
Homologene: 72199
Parp1
Name: poly (ADP-ribose) polymerase family, member 1
Synonyms: Adprt1, 5830444G22Rik, parp-1, Adprp, sPARP-1, PARP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 11545
HGNC: HGNC:270
Homologene: 1222
Copz1
Name: coatomer protein complex, subunit zeta 1
Synonyms: 5930435A22Rik, D4Ertd360e, nonclathrin coat protein zeta1-COP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 56447
VEGA: 15
HGNC: HGNC:2243
Homologene: 9366
Atf1
Name: activating transcription factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 11908
HGNC: HGNC:783
Homologene: 3790
Stat3
Name: signal transducer and activator of transcription 3
Synonyms: Aprf, 1110034C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 20848
Homologene: 7960
Rps24
Name: ribosomal protein S24
Synonyms: MRP S24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 20088
VEGA: 14
Homologene: 68148
Ptcd3
Name: pentatricopeptide repeat domain 3
Synonyms: 2810422B04Rik, 2610034F17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 69956
Homologene: 41211
Bcl6
Name: B cell leukemia/lymphoma 6
Synonyms: Bcl5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 12053
HGNC: HGNC:1001
Homologene: 7640
Nxpe2
Name: neurexophilin and PC-esterase domain family, member 2
Synonyms: 4432416J03Rik, Fam55b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 78252
Homologene: 87072
Rasa1
Name: RAS p21 protein activator 1
Synonyms: Gap, p120-rasGAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218397
HGNC: HGNC:9871
Homologene: 2168
Anxa5
Name: annexin A5
Synonyms: annexin V, Anx5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 11747
HGNC: HGNC:543
Homologene: 20312
Abcf3
Name: ATP-binding cassette, sub-family F (GCN20), member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 27406
HGNC: HGNC:72
Homologene: 22784
Acta2
Name: actin, alpha 2, smooth muscle, aorta
Synonyms: SMalphaA, alphaSMA, a-SMA, SMAalpha, 0610041G09Rik, Actvs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 11475
VEGA: 19
HGNC: HGNC:130
Homologene: 133938
Dock3
Name: dedicator of cyto-kinesis 3
Synonyms: Moca, PBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 208869
HGNC: HGNC:2989
Homologene: 21030
Serpina12
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
Synonyms: vaspin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 68054
Homologene: 23588
Bsn
Name: bassoon
Synonyms: presynaptic cytomatrix protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12217
HGNC: HGNC:1117
Homologene: 31161
Rsad2
Name: radical S-adenosyl methionine domain containing 2
Synonyms: viperin, cig5, 2510004L01Rik, Vig1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 58185
Homologene: 10969
Spock1
Name: sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1
Synonyms: testican 1, Ticn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20745
Homologene: 80193
Gm14226
Name: predicted gene 14226
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: null
HGNC: null
Homologene: null
Cdh15
Name: cadherin 15
Synonyms: Mcad, Cdh14, M cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 12555
HGNC: HGNC:1754
Homologene: 3622
Ip6k2
Name: inositol hexaphosphate kinase 2
Synonyms: Ihpk2, 1500005N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 76500
Homologene: 56929
Dmac2l
Name: distal membrane arm assembly complex 2 like
Synonyms: 1110015E18Rik, Atp5s, facyor B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 68055
VEGA: 12
Homologene: 12232
Cd300ld2
Name: CD300 molecule like family member D2
Synonyms: Gm11709
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 629970
HGNC: null
Homologene: null
Gm7337
Name: predicted gene 7337
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 654494
HGNC: null
Homologene: null
Olfr811
Name: olfactory receptor 811
Synonyms: MOR110-6, GA_x6K02T2PULF-11481207-11480248
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 258545
HGNC: null
Homologene: 133725
Atg4d
Name: autophagy related 4D, cysteine peptidase
Synonyms: Autl4, Apg4d, 9830134P12Rik, APG4-D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235040
VEGA: 9
Homologene: 13156
Zfp810
Name: zinc finger protein 810
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235050
VEGA: 9
HGNC: null
Homologene: 138299
Chad
Name: chondroadherin
Synonyms: SLRR4A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 12643
HGNC: HGNC:1909
Homologene: 36063
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 180,577,701 bp
  • A to T, chromosome 2 at 155,024,191 bp
  • G to A, chromosome 3 at 36,453,509 bp
  • T to C, chromosome 3 at 80,707,098 bp
  • G to A, chromosome 4 at 98,424,411 bp
  • A to G, chromosome 5 at 87,850,602 bp
  • T to A, chromosome 6 at 71,889,621 bp
  • G to C, chromosome 8 at 122,857,495 bp
  • T to C, chromosome 9 at 3,456,818 bp
  • T to A, chromosome 9 at 21,270,833 bp
  • T to C, chromosome 9 at 22,280,514 bp
  • T to C, chromosome 9 at 48,326,614 bp
  • C to T, chromosome 9 at 106,911,349 bp
  • T to C, chromosome 9 at 108,110,517 bp
  • T to C, chromosome 9 at 108,799,421 bp
  • A to G, chromosome 10 at 129,801,932 bp
  • A to T, chromosome 11 at 94,565,314 bp
  • A to T, chromosome 11 at 100,888,765 bp
  • G to A, chromosome 11 at 115,012,578 bp
  • T to C, chromosome 12 at 26,456,377 bp
  • T to A, chromosome 12 at 69,741,630 bp
  • T to C, chromosome 12 at 104,037,750 bp
  • A to T, chromosome 13 at 57,907,454 bp
  • A to G, chromosome 13 at 85,223,522 bp
  • A to G, chromosome 14 at 24,491,762 bp
  • A to G, chromosome 15 at 100,232,766 bp
  • A to G, chromosome 15 at 103,294,744 bp
  • A to G, chromosome 16 at 20,560,430 bp
  • A to G, chromosome 16 at 23,977,566 bp
  • G to A, chromosome 19 at 34,241,792 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1393 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039455-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.