Strain Name:
C57BL/6J-MtgxR1418Btlr/Mmmh
Stock Number:
039474-MU
Citation ID:
RRID:MMRRC_039474-MU
Other Names:
R1418 (G1), C57BL/6J-MtgxR1418Btlr
Major Collection:

Gene Information

Gene Symbol: Utrn [MGI:104631] (Mus musculus (mouse))
Name: utrophin
Synonyms: DRP, G-utrophin, Dmdl
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Nrp2 [MGI:1100492] (Mus musculus (mouse))
Name: neuropilin 2
Synonyms: NP2, Npn2, Npn-2, NP-2, 1110048P06Rik
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Elf1 [MGI:107180] (Mus musculus (mouse))
Name: E74-like factor 1
Synonyms: Elf-1
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Gli2 [MGI:95728] (Mus musculus (mouse))
Name: GLI-Kruppel family member GLI2
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Dusp1 [MGI:105120] (Mus musculus (mouse))
Name: dual specificity phosphatase 1
Synonyms: MKP1, mkp-1, 3CH134, erp, Ptpn16
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Smyd1 [MGI:104790] (Mus musculus (mouse))
Name: SET and MYND domain containing 1
Synonyms: Bop, 4632404M21Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Btbd11 [MGI:1921257] (Mus musculus (mouse))
Name: BTB (POZ) domain containing 11
Synonyms: 6330404E16Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 53,647,236 bp
  • C to T, chromosome 1 at 62,783,332 bp
  • G to A, chromosome 1 at 85,594,385 bp
  • T to G, chromosome 1 at 118,841,936 bp
  • T to C, chromosome 1 at 170,961,081 bp
  • T to A, chromosome 2 at 30,083,047 bp
  • A to T, chromosome 2 at 57,108,324 bp
  • A to G, chromosome 2 at 76,735,411 bp
  • G to T, chromosome 2 at 87,252,422 bp
  • A to T, chromosome 2 at 90,853,015 bp
  • GA to GAA, chromosome 2 at 90,917,709 bp
  • C to A, chromosome 2 at 104,777,431 bp
  • A to G, chromosome 2 at 130,600,290 bp
  • G to A, chromosome 2 at 157,241,854 bp
  • C to T, chromosome 2 at 174,345,214 bp
  • C to A, chromosome 3 at 36,180,095 bp
  • T to C, chromosome 3 at 38,890,813 bp
  • A to G, chromosome 3 at 81,961,468 bp
  • A to G, chromosome 3 at 90,238,738 bp
  • A to G, chromosome 3 at 95,470,399 bp
  • G to T, chromosome 3 at 100,927,298 bp
  • A to T, chromosome 3 at 101,018,775 bp
  • T to C, chromosome 3 at 130,007,423 bp
  • A to T, chromosome 4 at 118,387,779 bp
  • G to T, chromosome 4 at 141,767,741 bp
  • T to A, chromosome 4 at 143,616,034 bp
  • A to T, chromosome 5 at 14,678,130 bp
  • A to G, chromosome 5 at 34,078,975 bp
  • A to G, chromosome 5 at 34,217,246 bp
  • G to A, chromosome 5 at 41,819,471 bp
  • A to G, chromosome 5 at 90,464,202 bp
  • T to C, chromosome 5 at 114,418,575 bp
  • T to G, chromosome 5 at 121,423,734 bp
  • C to A, chromosome 5 at 125,638,249 bp
  • T to G, chromosome 6 at 52,179,151 bp
  • T to C, chromosome 6 at 65,597,429 bp
  • G to A, chromosome 6 at 71,262,167 bp
  • G to A, chromosome 6 at 106,344,870 bp
  • C to T, chromosome 6 at 121,838,676 bp
  • A to G, chromosome 7 at 16,869,545 bp
  • A to G, chromosome 7 at 30,576,960 bp
  • G to T, chromosome 7 at 46,274,615 bp
  • A to T, chromosome 7 at 46,938,010 bp
  • A to G, chromosome 7 at 98,716,830 bp
  • A to G, chromosome 7 at 119,857,935 bp
  • T to C, chromosome 8 at 72,100,387 bp
  • G to A, chromosome 8 at 105,379,110 bp
  • T to C, chromosome 8 at 114,282,361 bp
  • C to T, chromosome 9 at 21,947,789 bp
  • T to A, chromosome 9 at 40,074,472 bp
  • C to T, chromosome 10 at 12,713,350 bp
  • C to A, chromosome 10 at 85,645,578 bp
  • A to G, chromosome 10 at 116,319,470 bp
  • C to T, chromosome 10 at 128,083,298 bp
  • A to G, chromosome 11 at 4,409,005 bp
  • G to A, chromosome 11 at 29,545,002 bp
  • A to T, chromosome 11 at 35,809,740 bp
  • G to A, chromosome 11 at 54,909,596 bp
  • T to C, chromosome 11 at 60,778,032 bp
  • A to G, chromosome 11 at 61,523,086 bp
  • T to C, chromosome 11 at 72,401,421 bp
  • T to A, chromosome 11 at 98,241,785 bp
  • T to A, chromosome 11 at 101,285,740 bp
  • T to A, chromosome 11 at 118,074,023 bp
  • A to C, chromosome 12 at 21,239,585 bp
  • A to G, chromosome 12 at 21,239,589 bp
  • A to G, chromosome 12 at 80,692,885 bp
  • T to C, chromosome 12 at 111,627,837 bp
  • T to C, chromosome 13 at 22,592,879 bp
  • T to A, chromosome 13 at 40,717,204 bp
  • C to A, chromosome 13 at 109,950,387 bp
  • T to C, chromosome 13 at 112,690,180 bp
  • T to C, chromosome 14 at 74,804,269 bp
  • T to C, chromosome 14 at 79,560,775 bp
  • T to A, chromosome 15 at 11,286,804 bp
  • A to T, chromosome 15 at 36,265,266 bp
  • A to T, chromosome 15 at 82,405,905 bp
  • A to T, chromosome 15 at 97,718,973 bp
  • T to C, chromosome 16 at 90,769,466 bp
  • A to G, chromosome 17 at 26,508,319 bp
  • A to G, chromosome 17 at 27,894,577 bp
  • A to C, chromosome 17 at 31,328,241 bp
  • A to T, chromosome 17 at 65,859,148 bp
  • G to T, chromosome 18 at 20,397,430 bp
  • T to G, chromosome 18 at 25,339,699 bp
  • G to T, chromosome 18 at 50,130,187 bp
  • A to G, chromosome 18 at 87,757,334 bp
  • T to A, chromosome 19 at 40,914,359 bp
  • A to G, chromosome 19 at 47,130,077 bp
  • T to A, chromosome 19 at 47,671,505 bp
  • G to A, chromosome 19 at 57,371,162 bp
  • G to A, chromosome 19 at 57,935,705 bp
  • A to C, chromosome 19 at 58,238,417 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1418 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039474-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.