Strain Name    : B6.Cg-Mcoln3Va-J/Mmmh

Stock Number : 000395-MU

Gene Information

(provided by MGI)
Allele Symbol: Mcoln3Va-J
Name: varitint waddler Jackson
Chromosome: 3
Alteration at locus: Spontaneous Mutation
Gene Symbol: Mcoln3
Name: mucolipin 3
Chromosome: 3
Alteration at locus: Spontaneous Mutation
Genetic Alterations:
This is a spontaneous mutation.

Genotype Determination:

ES Cell Line: Not applicable

Phenotype

Homozygous phenotype:
- sensorineural hearing loss by two weeks of age
- white and diluted fur
- most homozygotes die in utero

Heterozygous phenotype:
- sensorineural hearing loss by two weeks of age
- white belly spot

Mammalian Phenotype Terms:
Allelic Composition: Mcoln3Va-J/Mcoln3Va-J (Genetic Background: B6.Cg-Mcoln3Va-J )

Allelic Composition: Mcoln3Va-J/Mcoln3Va-J (Genetic Background: B6C3Fe-a/a Hoxa13Hd Mcoln3Va-J/J )

Allelic Composition: Mcoln3Va-J/Mcoln3Va-J (Genetic Background: Not Specified )

Allelic Composition: Mcoln3Va-J/Mcoln3+ (Genetic Background: B6C3Fe-a/a Hoxa13Hd Mcoln3Va-J/J )

Allelic Composition: Mcoln3Va-J/Mcoln3+ (Genetic Background: Not Specified )

Allelic Composition: Mcoln3Va/Mcoln3Va-J (Genetic Background: Not Specified )

Strain of Origin: B6C3Fe-a/a Hox 13Hd

Strain genetic background: C57BL/6J

Suggested Control Mice:

  • Wildtype littermates

  • Sensorineural

Donor: Konrad Noben-Trauth, Ph.D., National Institutes of Health (NIDCD)

Primary Reference:

  • Di Palma F, Belyantseva IA, Kim HJ, Vogt TF, Kachar B, Noben-Trauth K. Mutations in Mcoln3 associated with deafness and pigmentation defects in varitint-waddler (Va) mice. Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14994-9. Epub 2002 Oct 25. (Medline PMID: 12403827)
  • Kim HJ, Jackson T, Noben-Trauth K. Genetic analyses of the mouse deafness mutations varitint-waddler (Va) and jerker (Espnje). J Assoc Res Otolaryngol. 2003 Mar;4(1):83-90. Epub 2002 Sep 18. (Medline PMID: 12209292 )

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Coat color: Black for Wildtype; Black with white belly spot for Heterozgyotes.

Other:


MMRRC Breeding System: Inbred

Breeding Scheme(s):

  • Heterozygous female x Wildtype male

Generation: N10 prior to arriving at MMRRC; F4 prior to arriving at MMRRC.

Overall Breeding Performance: Normal when bred Heterozygous x Wildtype.

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 16 weeks 16 weeks

Average litter size: 6 - 8

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
000395-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
000395-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.