Strain Name:
C57BL/6J-MtgxR1451Btlr/Mmmh
Stock Number:
039506-MU
Citation ID:
RRID:MMRRC_039506-MU
Other Names:
R1451 (G1), C57BL/6J-MtgxR1451Btlr
Major Collection:

Gene Information

Gene Symbol: Neurog2 [MGI:109619] (Mus musculus (mouse))
Name: neurogenin 2
Synonyms: ngn2, Math4A, Atoh4, bHLHa8
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Slc6a1 [MGI:95627] (Mus musculus (mouse))
Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 1
Synonyms: Xtrp1, Gabt, Gat1, Gabt1, XT-1, GAT-1
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Lrig3 [MGI:2443955] (Mus musculus (mouse))
Name: leucine-rich repeats and immunoglobulin-like domains 3
Synonyms: 9130004I02Rik, 9030421L11Rik, 9430095K15Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Arhgef12 [MGI:1916882] (Mus musculus (mouse))
Name: Rho guanine nucleotide exchange factor (GEF) 12
Synonyms: 2310014B11Rik, LARG
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Prex2 [MGI:1923385] (Mus musculus (mouse))
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: Depdc2, C030045D06Rik, 6230420N16Rik
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Gars [MGI:2449057] (Mus musculus (mouse))
Name: glycyl-tRNA synthetase
Synonyms: Gena201, GENA202, Sgrp23
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Lta4h [MGI:96836] (Mus musculus (mouse))
Name: leukotriene A4 hydrolase
Synonyms: LTA4 hydrodase
Chromosome: 10
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 11,156,259 bp
  • T to A, chromosome 1 at 92,511,795 bp
  • G to T, chromosome 2 at 36,421,865 bp
  • A to G, chromosome 2 at 181,380,191 bp
  • T to C, chromosome 3 at 53,009,113 bp
  • A to G, chromosome 3 at 127,633,841 bp
  • A to T, chromosome 4 at 8,189,327 bp
  • C to A, chromosome 4 at 118,621,072 bp
  • T to A, chromosome 5 at 108,798,067 bp
  • T to C, chromosome 5 at 115,088,365 bp
  • A to G, chromosome 6 at 3,565,628 bp
  • T to A, chromosome 6 at 29,419,377 bp
  • C to A, chromosome 6 at 42,361,451 bp
  • T to C, chromosome 6 at 55,053,123 bp
  • A to T, chromosome 6 at 86,430,339 bp
  • C to A, chromosome 6 at 114,307,795 bp
  • C to T, chromosome 6 at 119,236,824 bp
  • A to T, chromosome 6 at 124,521,641 bp
  • A to T, chromosome 6 at 124,625,494 bp
  • A to G, chromosome 7 at 5,059,528 bp
  • T to A, chromosome 7 at 23,928,850 bp
  • T to C, chromosome 7 at 24,105,218 bp
  • T to C, chromosome 7 at 28,137,157 bp
  • G to T, chromosome 7 at 101,421,991 bp
  • A to G, chromosome 8 at 41,354,012 bp
  • G to T, chromosome 8 at 71,386,019 bp
  • T to A, chromosome 8 at 86,557,693 bp
  • T to C, chromosome 8 at 122,615,395 bp
  • C to T, chromosome 9 at 37,417,711 bp
  • A to T, chromosome 9 at 38,606,938 bp
  • T to A, chromosome 9 at 39,737,316 bp
  • C to T, chromosome 9 at 42,992,578 bp
  • C to T, chromosome 9 at 61,924,802 bp
  • T to A, chromosome 10 at 7,126,830 bp
  • A to G, chromosome 10 at 93,480,728 bp
  • A to G, chromosome 10 at 103,202,515 bp
  • T to C, chromosome 10 at 126,010,057 bp
  • C to T, chromosome 11 at 58,455,532 bp
  • T to A, chromosome 11 at 67,856,181 bp
  • A to G, chromosome 11 at 110,127,447 bp
  • G to T, chromosome 11 at 115,990,884 bp
  • A to T, chromosome 11 at 120,362,022 bp
  • G to A, chromosome 12 at 35,078,984 bp
  • A to G, chromosome 12 at 102,405,892 bp
  • A to G, chromosome 13 at 8,339,621 bp
  • A to T, chromosome 13 at 21,574,287 bp
  • T to C, chromosome 14 at 32,376,631 bp
  • C to T, chromosome 14 at 56,668,421 bp
  • T to C, chromosome 15 at 101,998,829 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • A to G, chromosome 16 at 44,951,547 bp
  • A to G, chromosome 17 at 42,502,918 bp
  • A to T, chromosome 18 at 37,486,936 bp
  • A to T, chromosome 18 at 57,252,859 bp
  • T to C, chromosome 19 at 16,710,864 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1451 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039506-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.