Strain Name:
C57BL/6J-MtgxR1459Btlr/Mmmh
Stock Number:
039514-MU
Citation ID:
RRID:MMRRC_039514-MU
Other Names:
R1459 (G1), C57BL/6J-MtgxR1459Btlr
Major Collection:

Gene Information

Gene Symbol: Mitf [MGI:104554] (Mus musculus (mouse))
Name: melanogenesis associated transcription factor
Synonyms: wh, Gsfbcc2, mi, bHLHe32, BCC2
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Matr3 [MGI:1298379] (Mus musculus (mouse))
Name: matrin 3
Synonyms: D030046F20Rik, 1110061A14Rik, 2810017I02Rik
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Mrtfb [MGI:3050795] (Mus musculus (mouse))
Name: myocardin related transcription factor B
Synonyms: MRTF-B, Mrtfb, Gt4-1, Mkl2
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Cd86 [MGI:101773] (Mus musculus (mouse))
Name: CD86 antigen
Synonyms: MB7-2, B7-2, Cd28l2, Ly58, Ly-58, B70, B7.2
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Prkd3 [MGI:1922542] (Mus musculus (mouse))
Name: protein kinase D3
Synonyms: Prkcn, PKD3, 4930557O20Rik, 5730497N19Rik
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Adcyap1 [MGI:105094] (Mus musculus (mouse))
Name: adenylate cyclase activating polypeptide 1
Synonyms: PACAP
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Lrp2 [MGI:95794] (Mus musculus (mouse))
Name: low density lipoprotein receptor-related protein 2
Synonyms: b2b1625.2Clo, Megalin, D230004K18Rik, Gp330
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 171,851,943 bp
  • T to C, chromosome 1 at 188,862,851 bp
  • G to A, chromosome 2 at 4,744,428 bp
  • G to T, chromosome 2 at 25,092,546 bp
  • T to G, chromosome 2 at 32,297,795 bp
  • T to A, chromosome 2 at 69,460,477 bp
  • T to A, chromosome 2 at 69,483,394 bp
  • A to G, chromosome 2 at 76,555,065 bp
  • T to A, chromosome 2 at 90,690,150 bp
  • C to A, chromosome 2 at 110,880,829 bp
  • C to T, chromosome 2 at 130,614,797 bp
  • C to A, chromosome 2 at 164,354,917 bp
  • A to G, chromosome 3 at 146,192,224 bp
  • T to A, chromosome 3 at 157,972,310 bp
  • C to T, chromosome 4 at 62,532,341 bp
  • T to C, chromosome 4 at 86,425,865 bp
  • T to G, chromosome 4 at 99,912,547 bp
  • C to T, chromosome 4 at 119,255,613 bp
  • T to A, chromosome 4 at 136,329,444 bp
  • T to C, chromosome 4 at 141,167,762 bp
  • T to A, chromosome 4 at 155,854,019 bp
  • T to A, chromosome 5 at 8,702,920 bp
  • T to C, chromosome 5 at 8,918,662 bp
  • T to A, chromosome 5 at 21,207,238 bp
  • A to G, chromosome 5 at 67,400,075 bp
  • T to A, chromosome 5 at 112,915,012 bp
  • A to G, chromosome 5 at 124,743,686 bp
  • A to G, chromosome 6 at 98,010,467 bp
  • T to A, chromosome 6 at 113,710,070 bp
  • T to A, chromosome 6 at 129,538,672 bp
  • T to A, chromosome 6 at 149,582,152 bp
  • A to T, chromosome 7 at 85,406,700 bp
  • C to A, chromosome 7 at 87,037,794 bp
  • G to T, chromosome 7 at 98,131,842 bp
  • T to A, chromosome 7 at 108,924,348 bp
  • C to T, chromosome 7 at 118,510,037 bp
  • G to A, chromosome 8 at 48,235,971 bp
  • T to A, chromosome 8 at 70,244,064 bp
  • T to C, chromosome 8 at 105,381,799 bp
  • A to T, chromosome 9 at 119,223,761 bp
  • T to C, chromosome 10 at 3,260,240 bp
  • T to A, chromosome 10 at 51,491,587 bp
  • T to A, chromosome 10 at 61,198,804 bp
  • C to G, chromosome 10 at 77,042,535 bp
  • T to C, chromosome 10 at 91,062,160 bp
  • T to C, chromosome 10 at 93,868,949 bp
  • A to T, chromosome 10 at 125,230,620 bp
  • C to A, chromosome 11 at 3,927,516 bp
  • A to T, chromosome 11 at 60,759,362 bp
  • A to G, chromosome 11 at 67,662,076 bp
  • C to T, chromosome 11 at 106,817,955 bp
  • G to A, chromosome 11 at 106,852,507 bp
  • A to T, chromosome 11 at 115,979,111 bp
  • A to G, chromosome 12 at 8,006,047 bp
  • A to G, chromosome 12 at 8,011,937 bp
  • A to G, chromosome 12 at 32,204,984 bp
  • T to A, chromosome 12 at 76,611,883 bp
  • A to G, chromosome 12 at 111,296,300 bp
  • C to T, chromosome 13 at 27,709,257 bp
  • A to T, chromosome 13 at 48,586,697 bp
  • T to A, chromosome 13 at 92,661,187 bp
  • G to A, chromosome 14 at 31,017,846 bp
  • T to A, chromosome 14 at 59,244,778 bp
  • A to C, chromosome 15 at 30,847,299 bp
  • G to A, chromosome 15 at 83,306,937 bp
  • T to A, chromosome 16 at 3,937,589 bp
  • T to A, chromosome 16 at 4,648,609 bp
  • T to C, chromosome 16 at 13,401,569 bp
  • G to A, chromosome 16 at 14,304,516 bp
  • T to C, chromosome 16 at 33,236,329 bp
  • T to A, chromosome 16 at 36,628,988 bp
  • C to T, chromosome 16 at 56,024,340 bp
  • T to C, chromosome 16 at 88,671,414 bp
  • T to C, chromosome 16 at 91,655,342 bp
  • T to A, chromosome 17 at 30,982,258 bp
  • T to C, chromosome 17 at 30,986,504 bp
  • T to C, chromosome 17 at 78,971,367 bp
  • G to T, chromosome 17 at 84,610,775 bp
  • A to G, chromosome 17 at 87,678,343 bp
  • T to C, chromosome 17 at 93,200,122 bp
  • T to A, chromosome 18 at 35,584,656 bp
  • T to A, chromosome 18 at 42,737,990 bp
  • A to G, chromosome 19 at 5,651,664 bp
  • T to A, chromosome 19 at 36,845,531 bp
  • T to A, chromosome 19 at 45,021,454 bp
  • T to C, chromosome 19 at 56,384,130 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1459 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039514-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.