Strain Name:
C57BL/6J-MtgxR1463Btlr/Mmmh
Stock Number:
039517-MU
Citation ID:
RRID:MMRRC_039517-MU
Other Names:
R1463 (G1), C57BL/6J-MtgxR1463Btlr
Major Collection:

Gene Information

Gene Symbol: Myo1e [MGI:106621] (Mus musculus (mouse))
Name: myosin IE
Synonyms: 9130023P14Rik, 2310020N23Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Yes1 [MGI:99147] (Mus musculus (mouse))
Name: YES proto-oncogene 1, Src family tyrosine kinase
Synonyms: Yes
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Plcd3 [MGI:107451] (Mus musculus (mouse))
Name: phospholipase C, delta 3
Synonyms: 2610205J15Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Abcc8 [MGI:1352629] (Mus musculus (mouse))
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 8
Synonyms: SUR1, D930031B21Rik, Sur
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Dock6 [MGI:1914789] (Mus musculus (mouse))
Name: dedicator of cytokinesis 6
Synonyms: C330023D02Rik, 2410095B20Rik, 4931431C02Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Mier3 [MGI:2442317] (Mus musculus (mouse))
Name: MIER family member 3
Synonyms: D130064H19Rik
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Atg14 [MGI:1261775] (Mus musculus (mouse))
Name: autophagy related 14
Synonyms: Barkor, D14Ertd436e, D14Ertd114e
Chromosome: 14
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 26,682,141 bp
  • T to C, chromosome 1 at 43,113,229 bp
  • T to A, chromosome 1 at 65,048,838 bp
  • A to T, chromosome 1 at 65,363,277 bp
  • T to C, chromosome 1 at 72,300,696 bp
  • A to G, chromosome 1 at 75,146,520 bp
  • T to A, chromosome 1 at 93,499,315 bp
  • A to G, chromosome 1 at 132,521,137 bp
  • A to G, chromosome 1 at 151,361,540 bp
  • A to G, chromosome 1 at 151,807,510 bp
  • C to T, chromosome 1 at 164,257,197 bp
  • G to C, chromosome 1 at 170,150,383 bp
  • A to G, chromosome 1 at 173,203,367 bp
  • A to T, chromosome 1 at 189,654,739 bp
  • T to C, chromosome 1 at 191,328,289 bp
  • C to A, chromosome 2 at 31,887,411 bp
  • T to A, chromosome 2 at 32,400,862 bp
  • A to G, chromosome 2 at 60,368,757 bp
  • T to C, chromosome 2 at 69,885,246 bp
  • A to T, chromosome 2 at 76,827,515 bp
  • G to A, chromosome 2 at 114,049,529 bp
  • G to A, chromosome 2 at 121,406,446 bp
  • C to T, chromosome 2 at 122,123,735 bp
  • T to C, chromosome 2 at 153,330,153 bp
  • C to T, chromosome 2 at 170,418,664 bp
  • A to G, chromosome 3 at 49,755,405 bp
  • G to A, chromosome 3 at 98,162,525 bp
  • A to G, chromosome 3 at 123,374,073 bp
  • A to T, chromosome 3 at 151,510,596 bp
  • A to G, chromosome 3 at 153,857,570 bp
  • T to G, chromosome 4 at 88,602,956 bp
  • T to A, chromosome 4 at 103,087,418 bp
  • C to A, chromosome 5 at 5,452,073 bp
  • A to G, chromosome 5 at 7,179,372 bp
  • T to A, chromosome 5 at 30,935,994 bp
  • T to A, chromosome 5 at 32,651,702 bp
  • C to T, chromosome 5 at 147,306,660 bp
  • G to T, chromosome 6 at 118,593,994 bp
  • A to G, chromosome 6 at 124,311,447 bp
  • A to T, chromosome 6 at 145,225,061 bp
  • C to T, chromosome 7 at 7,296,764 bp
  • C to A, chromosome 7 at 21,360,730 bp
  • T to C, chromosome 7 at 46,154,512 bp
  • T to G, chromosome 7 at 49,535,962 bp
  • C to T, chromosome 7 at 122,992,453 bp
  • T to A, chromosome 7 at 131,109,637 bp
  • C to A, chromosome 7 at 131,316,642 bp
  • T to C, chromosome 7 at 133,675,859 bp
  • T to C, chromosome 7 at 141,859,080 bp
  • T to A, chromosome 8 at 57,652,858 bp
  • T to C, chromosome 8 at 124,030,908 bp
  • G to A, chromosome 8 at 125,942,137 bp
  • C to T, chromosome 9 at 9,673,796 bp
  • T to C, chromosome 9 at 21,831,906 bp
  • G to A, chromosome 9 at 70,338,756 bp
  • A to T, chromosome 9 at 104,178,940 bp
  • A to G, chromosome 10 at 7,446,557 bp
  • T to C, chromosome 10 at 60,062,516 bp
  • C to A, chromosome 10 at 126,993,921 bp
  • T to C, chromosome 11 at 3,501,147 bp
  • C to T, chromosome 11 at 8,916,302 bp
  • A to G, chromosome 11 at 58,598,121 bp
  • A to T, chromosome 11 at 82,981,698 bp
  • C to T, chromosome 11 at 94,116,837 bp
  • A to G, chromosome 11 at 96,963,456 bp
  • A to G, chromosome 11 at 103,078,373 bp
  • A to T, chromosome 11 at 110,314,558 bp
  • A to T, chromosome 12 at 32,974,333 bp
  • GCTCAGTGTATC to GC, chromosome 12 at 40,816,325 bp
  • A to T, chromosome 12 at 84,680,363 bp
  • T to A, chromosome 12 at 104,138,710 bp
  • T to C, chromosome 13 at 33,255,116 bp
  • T to C, chromosome 13 at 46,929,612 bp
  • G to A, chromosome 13 at 60,801,275 bp
  • A to G, chromosome 13 at 111,711,755 bp
  • T to C, chromosome 14 at 20,550,190 bp
  • T to C, chromosome 14 at 47,548,994 bp
  • T to A, chromosome 14 at 55,778,939 bp
  • T to C, chromosome 14 at 60,788,146 bp
  • T to A, chromosome 14 at 68,085,021 bp
  • A to T, chromosome 14 at 118,267,482 bp
  • T to C, chromosome 15 at 36,539,298 bp
  • T to A, chromosome 15 at 66,919,271 bp
  • A to G, chromosome 15 at 80,379,054 bp
  • A to G, chromosome 16 at 11,315,202 bp
  • T to A, chromosome 16 at 17,559,873 bp
  • C to T, chromosome 16 at 39,011,211 bp
  • A to T, chromosome 17 at 20,687,732 bp
  • C to T, chromosome 17 at 27,117,154 bp
  • G to A, chromosome 17 at 34,034,160 bp
  • A to G, chromosome 17 at 36,366,720 bp
  • C to T, chromosome 17 at 58,932,445 bp
  • G to A, chromosome 18 at 12,191,830 bp
  • A to G, chromosome 18 at 22,516,753 bp
  • T to C, chromosome 18 at 32,133,438 bp
  • T to C, chromosome 18 at 58,010,380 bp
  • A to C, chromosome 19 at 6,912,455 bp
  • C to T, chromosome 19 at 12,422,888 bp
  • A to G, chromosome 19 at 12,648,103 bp
  • A to G, chromosome 19 at 37,139,100 bp
  • T to C, chromosome 19 at 46,271,545 bp
  • T to A, chromosome 19 at 56,930,151 bp
  • C to T, chromosome X at 153,384,681 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1463 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039517-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.