Strain Name:
C57BL/6J-MtgxR1465Btlr/Mmmh
Stock Number:
039519-MU
Citation ID:
RRID:MMRRC_039519-MU
Other Names:
R1465 (G1), C57BL/6J-MtgxR1465Btlr
Major Collection:

Gene Information

Gene Symbol: Dnmt3a [MGI:1261827] (Mus musculus (mouse))
Name: DNA methyltransferase 3A
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Hlcs [MGI:894646] (Mus musculus (mouse))
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: 410I21.SP6, D16Jhu34
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Ntrk3 [MGI:97385] (Mus musculus (mouse))
Name: neurotrophic tyrosine kinase, receptor, type 3
Synonyms: TrkC
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Acvr1c [MGI:2661081] (Mus musculus (mouse))
Name: activin A receptor, type IC
Synonyms: Alk-7, ALK7
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Faah [MGI:109609] (Mus musculus (mouse))
Name: fatty acid amide hydrolase
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Macf1 [MGI:108559] (Mus musculus (mouse))
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Dock1 [MGI:2429765] (Mus musculus (mouse))
Name: dedicator of cytokinesis 1
Synonyms: 9130006G06Rik, Dock180, b2b3190Clo, D630004B07Rik
Chromosome: 7
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 5,095,688 bp
  • A to G, chromosome 1 at 21,469,468 bp
  • G to T, chromosome 1 at 52,790,094 bp
  • T to C, chromosome 1 at 54,528,555 bp
  • A to G, chromosome 1 at 75,428,484 bp
  • A to T, chromosome 1 at 82,497,822 bp
  • G to C, chromosome 1 at 88,257,802 bp
  • A to T, chromosome 1 at 107,155,843 bp
  • A to T, chromosome 1 at 164,198,833 bp
  • T to C, chromosome 2 at 22,577,927 bp
  • C to T, chromosome 2 at 25,843,347 bp
  • G to T, chromosome 2 at 29,140,389 bp
  • A to G, chromosome 2 at 31,520,416 bp
  • C to T, chromosome 2 at 41,111,059 bp
  • A to G, chromosome 2 at 58,284,961 bp
  • A to C, chromosome 2 at 116,058,670 bp
  • C to A, chromosome 2 at 132,872,075 bp
  • T to A, chromosome 2 at 150,239,047 bp
  • A to G, chromosome 2 at 151,030,829 bp
  • G to A, chromosome 2 at 152,829,950 bp
  • G to A, chromosome 2 at 154,271,021 bp
  • A to G, chromosome 3 at 88,087,309 bp
  • T to C, chromosome 3 at 116,687,334 bp
  • T to C, chromosome 3 at 116,771,372 bp
  • A to G, chromosome 4 at 11,296,034 bp
  • A to G, chromosome 4 at 43,820,723 bp
  • G to A, chromosome 4 at 45,273,197 bp
  • A to C, chromosome 4 at 72,139,831 bp
  • A to G, chromosome 4 at 96,013,256 bp
  • A to T, chromosome 4 at 99,037,520 bp
  • A to T, chromosome 4 at 115,999,558 bp
  • A to T, chromosome 4 at 123,493,154 bp
  • C to A, chromosome 4 at 141,805,840 bp
  • A to C, chromosome 4 at 148,013,901 bp
  • G to T, chromosome 4 at 148,525,993 bp
  • A to T, chromosome 5 at 90,550,341 bp
  • C to T, chromosome 5 at 109,220,329 bp
  • T to C, chromosome 5 at 110,209,878 bp
  • T to A, chromosome 5 at 138,125,208 bp
  • T to C, chromosome 5 at 149,214,032 bp
  • T to C, chromosome 6 at 41,616,106 bp
  • T to C, chromosome 6 at 51,909,368 bp
  • T to A, chromosome 6 at 52,203,791 bp
  • T to C, chromosome 6 at 52,727,194 bp
  • A to G, chromosome 7 at 10,100,322 bp
  • A to C, chromosome 7 at 14,416,283 bp
  • T to G, chromosome 7 at 19,730,116 bp
  • C to A, chromosome 7 at 44,191,361 bp
  • T to C, chromosome 7 at 78,356,014 bp
  • C to A, chromosome 7 at 83,895,582 bp
  • T to C, chromosome 7 at 104,644,946 bp
  • T to A, chromosome 7 at 119,801,358 bp
  • T to C, chromosome 7 at 127,788,340 bp
  • A to G, chromosome 7 at 134,782,409 bp
  • C to A, chromosome 7 at 143,617,931 bp
  • A to T, chromosome 8 at 46,025,235 bp
  • T to A, chromosome 8 at 81,768,157 bp
  • T to C, chromosome 8 at 105,338,914 bp
  • T to C, chromosome 8 at 105,381,040 bp
  • A to G, chromosome 9 at 24,669,322 bp
  • A to G, chromosome 9 at 50,740,566 bp
  • A to G, chromosome 9 at 79,898,307 bp
  • C to T, chromosome 9 at 106,157,607 bp
  • T to C, chromosome 9 at 108,071,466 bp
  • G to T, chromosome 10 at 70,570,968 bp
  • G to T, chromosome 10 at 79,631,302 bp
  • TCGC to TC, chromosome 11 at 3,146,254 bp
  • G to T, chromosome 11 at 9,399,303 bp
  • G to A, chromosome 11 at 23,002,922 bp
  • T to A, chromosome 11 at 43,705,556 bp
  • T to C, chromosome 11 at 48,801,759 bp
  • A to G, chromosome 11 at 51,654,913 bp
  • A to T, chromosome 11 at 102,153,329 bp
  • A to G, chromosome 11 at 120,408,781 bp
  • A to G, chromosome 12 at 3,866,088 bp
  • T to C, chromosome 12 at 8,011,421 bp
  • A to G, chromosome 12 at 72,500,759 bp
  • T to C, chromosome 12 at 84,813,300 bp
  • T to C, chromosome 12 at 118,038,695 bp
  • A to G, chromosome 13 at 92,368,307 bp
  • T to C, chromosome 14 at 24,154,696 bp
  • C to T, chromosome 14 at 30,956,887 bp
  • A to G, chromosome 14 at 50,453,177 bp
  • T to A, chromosome 14 at 75,107,389 bp
  • T to A, chromosome 14 at 88,469,237 bp
  • T to C, chromosome 14 at 96,240,213 bp
  • T to C, chromosome 14 at 101,447,688 bp
  • T to C, chromosome 15 at 78,907,345 bp
  • T to A, chromosome 15 at 80,648,411 bp
  • A to T, chromosome 15 at 82,403,928 bp
  • G to T, chromosome 16 at 5,201,528 bp
  • T to C, chromosome 16 at 46,153,848 bp
  • T to C, chromosome 16 at 94,268,292 bp
  • T to C, chromosome 17 at 7,292,867 bp
  • G to T, chromosome 17 at 55,610,935 bp
  • G to A, chromosome 17 at 74,623,858 bp
  • T to A, chromosome 17 at 80,367,301 bp
  • A to T, chromosome 17 at 84,436,676 bp
  • G to T, chromosome 18 at 10,072,863 bp
  • G to A, chromosome 18 at 37,504,697 bp
  • A to G, chromosome 18 at 60,818,954 bp
  • T to C, chromosome 18 at 67,783,984 bp
  • A to T, chromosome 18 at 76,876,645 bp
  • A to G, chromosome 18 at 77,380,573 bp
  • T to G, chromosome 18 at 87,757,258 bp
  • T to C, chromosome 19 at 6,938,444 bp
  • T to A, chromosome 19 at 13,234,901 bp
  • T to C, chromosome 19 at 34,316,613 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1465 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039519-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.