Strain Name:
C57BL/6J-MtgxR1496Btlr/Mmmh
Stock Number:
039547-MU
Citation ID:
RRID:MMRRC_039547-MU
Other Names:
R1496 (G1), C57BL/6J-MtgxR1496Btlr
Major Collection:

Gene Information

Gene Symbol: Zdhhc17 [MGI:2445110] (Mus musculus (mouse))
Name: zinc finger, DHHC domain containing 17
Synonyms: D130071N24Rik, A230053P19Rik, Hip14
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Ptprz1 [MGI:97816] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, receptor type Z, polypeptide 1
Synonyms: Ptprz, phosphacan, DSD-1-PG, PTPzeta, PTPbeta, Rptpbeta, RPTPz, Ptpz
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Sptbn1 [MGI:98388] (Mus musculus (mouse))
Name: spectrin beta, non-erythrocytic 1
Synonyms: Spnb2, 9930031C03Rik, elf3, Spnb-2, non-erythrocytic, beta fodrin, brain spectrin, spectrin G, elf1
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Elavl3 [MGI:109157] (Mus musculus (mouse))
Name: ELAV like RNA binding protein 3
Synonyms: mHuC, 2600009P04Rik, Huc
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Sgpl1 [MGI:1261415] (Mus musculus (mouse))
Name: sphingosine phosphate lyase 1
Synonyms: D10Xrf456
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Mthfd1l [MGI:1924836] (Mus musculus (mouse))
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Synonyms: 2410004L15Rik, Fthfsdc1
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Shroom3 [MGI:1351655] (Mus musculus (mouse))
Name: shroom family member 3
Synonyms: Shrm3, Shrm, D5Ertd287e
Chromosome: 5
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 85,663,521 bp
  • A to T, chromosome 1 at 88,275,050 bp
  • T to C, chromosome 1 at 171,125,817 bp
  • T to C, chromosome 1 at 172,193,855 bp
  • T to C, chromosome 1 at 177,772,194 bp
  • G to A, chromosome 2 at 24,678,035 bp
  • G to A, chromosome 2 at 35,718,791 bp
  • A to T, chromosome 2 at 42,323,662 bp
  • T to G, chromosome 2 at 52,328,734 bp
  • G to A, chromosome 2 at 66,526,888 bp
  • A to T, chromosome 2 at 70,512,773 bp
  • T to C, chromosome 2 at 73,679,607 bp
  • A to T, chromosome 2 at 86,553,752 bp
  • T to G, chromosome 2 at 87,802,868 bp
  • A to G, chromosome 2 at 89,630,014 bp
  • T to C, chromosome 2 at 105,832,161 bp
  • T to C, chromosome 2 at 125,309,495 bp
  • G to A, chromosome 2 at 128,826,393 bp
  • T to C, chromosome 3 at 20,263,532 bp
  • T to G, chromosome 3 at 22,190,951 bp
  • C to A, chromosome 3 at 88,384,338 bp
  • A to G, chromosome 3 at 96,905,210 bp
  • T to C, chromosome 3 at 154,939,658 bp
  • T to A, chromosome 4 at 14,506,489 bp
  • T to A, chromosome 4 at 150,933,104 bp
  • T to C, chromosome 5 at 33,729,750 bp
  • A to G, chromosome 5 at 92,309,823 bp
  • T to C, chromosome 5 at 92,942,834 bp
  • T to A, chromosome 5 at 110,650,078 bp
  • GAA to GA, chromosome 5 at 124,459,885 bp
  • T to C, chromosome 5 at 129,998,544 bp
  • G to T, chromosome 5 at 143,507,338 bp
  • T to A, chromosome 6 at 18,849,659 bp
  • C to T, chromosome 6 at 23,049,524 bp
  • A to G, chromosome 6 at 42,356,312 bp
  • A to T, chromosome 6 at 101,150,969 bp
  • A to G, chromosome 7 at 29,776,614 bp
  • G to A, chromosome 7 at 51,583,775 bp
  • T to A, chromosome 7 at 79,100,804 bp
  • T to A, chromosome 7 at 90,130,651 bp
  • A to G, chromosome 8 at 35,469,181 bp
  • A to G, chromosome 8 at 45,033,390 bp
  • C to T, chromosome 8 at 121,596,600 bp
  • A to T, chromosome 9 at 4,330,276 bp
  • T to A, chromosome 9 at 22,026,165 bp
  • A to G, chromosome 9 at 46,310,254 bp
  • T to A, chromosome 9 at 57,119,158 bp
  • A to T, chromosome 9 at 123,810,347 bp
  • G to A, chromosome 10 at 4,047,986 bp
  • A to T, chromosome 10 at 6,935,173 bp
  • A to G, chromosome 10 at 19,601,445 bp
  • T to C, chromosome 10 at 61,102,589 bp
  • T to A, chromosome 10 at 78,955,848 bp
  • T to C, chromosome 10 at 82,659,538 bp
  • A to G, chromosome 10 at 100,538,966 bp
  • T to G, chromosome 10 at 110,946,210 bp
  • T to C, chromosome 10 at 127,539,011 bp
  • A to G, chromosome 10 at 128,512,428 bp
  • T to C, chromosome 11 at 5,874,175 bp
  • T to A, chromosome 11 at 8,941,077 bp
  • T to C, chromosome 11 at 30,121,498 bp
  • T to A, chromosome 11 at 59,031,036 bp
  • A to G, chromosome 11 at 74,053,824 bp
  • T to C, chromosome 11 at 94,505,561 bp
  • T to C, chromosome 11 at 102,361,171 bp
  • A to T, chromosome 13 at 21,318,920 bp
  • G to A, chromosome 13 at 22,805,764 bp
  • T to C, chromosome 13 at 25,005,034 bp
  • T to A, chromosome 13 at 43,094,990 bp
  • C to T, chromosome 13 at 97,965,546 bp
  • C to T, chromosome 14 at 26,710,248 bp
  • A to G, chromosome 14 at 32,835,631 bp
  • T to G, chromosome 14 at 55,487,650 bp
  • A to T, chromosome 14 at 65,624,133 bp
  • A to G, chromosome 14 at 73,197,299 bp
  • G to T, chromosome 15 at 65,876,521 bp
  • G to T, chromosome 15 at 97,686,483 bp
  • T to A, chromosome 16 at 14,448,434 bp
  • A to G, chromosome 16 at 16,780,614 bp
  • T to A, chromosome 16 at 19,530,383 bp
  • T to C, chromosome 16 at 88,562,401 bp
  • T to A, chromosome 17 at 24,813,052 bp
  • C to T, chromosome 17 at 34,740,021 bp
  • G to T, chromosome 18 at 6,992,330 bp
  • T to C, chromosome 18 at 32,261,297 bp
  • T to C, chromosome 18 at 32,412,704 bp
  • T to G, chromosome 18 at 78,859,912 bp
  • A to G, chromosome 19 at 3,478,373 bp
  • A to G, chromosome 19 at 6,007,498 bp
  • A to T, chromosome 19 at 8,803,350 bp
  • A to G, chromosome 19 at 20,868,355 bp
  • C to G, chromosome 19 at 26,631,101 bp
  • T to C, chromosome 19 at 42,053,050 bp
  • G to A, chromosome 19 at 46,904,978 bp
  • G to T, chromosome X at 20,859,704 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1496 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039547-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.