Strain Name:
C57BL/6J-MtgxR1500Btlr/Mmmh
Stock Number:
039551-MU
Citation ID:
RRID:MMRRC_039551-MU
Other Names:
R1500 (G1), C57BL/6J-MtgxR1500Btlr
Major Collection:

Gene Information

Gene Symbol: Lamb1 [MGI:96743] (Mus musculus (mouse))
Name: laminin B1
Synonyms: C80098, C81607, D130003D08Rik, Lamb1-1, Lamb-1
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Ank2 [MGI:88025] (Mus musculus (mouse))
Name: ankyrin 2, brain
Synonyms: Gm4392, ankyrin B, Ankyrin-2, Ank-2, Ankyrin-B
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Nae1 [MGI:2384561] (Mus musculus (mouse))
Name: NEDD8 activating enzyme E1 subunit 1
Synonyms: Appbp1
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Gldc [MGI:1341155] (Mus musculus (mouse))
Name: glycine decarboxylase
Synonyms: D030049L12Rik, b2b2679Clo, D19Wsu57e
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: H2-D1 [MGI:95896] (Mus musculus (mouse))
Name: histocompatibility 2, D region locus 1
Synonyms: H-2D
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Ylpm1 [MGI:1926195] (Mus musculus (mouse))
Name: YLP motif containing 1
Synonyms: ZAP, Zap3, A930013E17Rik
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Arhgef6 [MGI:1920591] (Mus musculus (mouse))
Name: Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
Synonyms: 4930592P22Rik, alpha-PIX, 1600028C08Rik, 1700038J06Rik
Chromosome: X
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 66,521,581 bp
  • C to A, chromosome 1 at 88,412,293 bp
  • G to T, chromosome 1 at 89,145,488 bp
  • T to C, chromosome 1 at 93,397,328 bp
  • T to C, chromosome 1 at 140,100,876 bp
  • A to T, chromosome 1 at 153,408,379 bp
  • A to G, chromosome 1 at 169,690,414 bp
  • G to A, chromosome 2 at 25,677,119 bp
  • A to T, chromosome 2 at 32,576,679 bp
  • A to T, chromosome 2 at 36,377,621 bp
  • G to T, chromosome 2 at 76,745,528 bp
  • T to A, chromosome 2 at 88,677,875 bp
  • A to T, chromosome 2 at 121,140,007 bp
  • A to T, chromosome 2 at 137,115,638 bp
  • A to G, chromosome 2 at 155,499,046 bp
  • A to C, chromosome 2 at 181,991,233 bp
  • T to C, chromosome 3 at 33,014,980 bp
  • A to G, chromosome 3 at 36,182,580 bp
  • G to A, chromosome 3 at 97,582,805 bp
  • T to C, chromosome 3 at 97,663,947 bp
  • T to C, chromosome 3 at 116,027,140 bp
  • A to G, chromosome 3 at 126,932,982 bp
  • G to T, chromosome 4 at 53,735,065 bp
  • C to T, chromosome 4 at 58,070,239 bp
  • T to C, chromosome 4 at 129,078,961 bp
  • C to T, chromosome 4 at 138,305,275 bp
  • T to A, chromosome 5 at 32,901,817 bp
  • T to C, chromosome 5 at 33,661,308 bp
  • T to C, chromosome 5 at 137,210,501 bp
  • A to G, chromosome 6 at 34,872,725 bp
  • T to A, chromosome 6 at 89,845,501 bp
  • T to A, chromosome 6 at 132,207,476 bp
  • C to T, chromosome 7 at 5,125,442 bp
  • T to A, chromosome 7 at 19,758,964 bp
  • A to G, chromosome 7 at 26,567,891 bp
  • T to C, chromosome 7 at 106,790,821 bp
  • A to G, chromosome 8 at 33,575,092 bp
  • A to G, chromosome 8 at 66,468,390 bp
  • A to G, chromosome 8 at 80,995,770 bp
  • A to T, chromosome 8 at 104,523,584 bp
  • T to G, chromosome 8 at 109,948,542 bp
  • A to T, chromosome 9 at 19,319,316 bp
  • AGAGGAGGAGGAGGAGGAGGAGGAGGA to AGAGGAGGAGGAGGAGGAGGAGGA, chromosome 9 at 20,873,717 bp
  • A to C, chromosome 9 at 22,457,455 bp
  • G to T, chromosome 9 at 39,873,411 bp
  • A to C, chromosome 9 at 88,479,896 bp
  • T to C, chromosome 10 at 80,394,586 bp
  • G to T, chromosome 10 at 121,391,491 bp
  • T to A, chromosome 11 at 6,520,811 bp
  • T to C, chromosome 11 at 29,482,713 bp
  • T to C, chromosome 11 at 53,372,378 bp
  • T to C, chromosome 11 at 58,612,505 bp
  • C to A, chromosome 11 at 78,284,132 bp
  • T to A, chromosome 11 at 84,293,984 bp
  • A to G, chromosome 11 at 86,661,200 bp
  • C to T, chromosome 11 at 98,428,978 bp
  • T to C, chromosome 11 at 98,518,695 bp
  • T to G, chromosome 11 at 105,347,725 bp
  • T to C, chromosome 11 at 118,101,053 bp
  • T to C, chromosome 12 at 31,298,949 bp
  • A to T, chromosome 12 at 61,839,741 bp
  • T to C, chromosome 12 at 85,014,996 bp
  • G to A, chromosome 12 at 110,636,509 bp
  • T to C, chromosome 12 at 110,794,030 bp
  • A to T, chromosome 12 at 118,012,829 bp
  • C to G, chromosome 13 at 27,121,706 bp
  • G to A, chromosome 13 at 66,995,408 bp
  • T to C, chromosome 13 at 81,435,548 bp
  • T to A, chromosome 13 at 104,312,881 bp
  • G to A, chromosome 13 at 111,255,320 bp
  • A to G, chromosome 14 at 28,271,660 bp
  • T to C, chromosome 14 at 31,316,758 bp
  • G to A, chromosome 14 at 37,062,134 bp
  • T to C, chromosome 15 at 44,545,494 bp
  • T to C, chromosome 15 at 55,617,555 bp
  • A to G, chromosome 15 at 68,928,786 bp
  • A to G, chromosome 15 at 101,530,224 bp
  • T to C, chromosome 16 at 20,644,242 bp
  • T to C, chromosome 16 at 55,233,572 bp
  • A to T, chromosome 16 at 63,595,662 bp
  • C to T, chromosome 17 at 18,256,980 bp
  • A to G, chromosome 17 at 31,111,279 bp
  • G to A, chromosome 17 at 35,263,588 bp
  • T to C, chromosome 17 at 46,986,689 bp
  • T to G, chromosome 19 at 11,183,861 bp
  • A to C, chromosome 19 at 30,113,825 bp
  • T to C, chromosome X at 57,338,562 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1500 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039551-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.