Strain Name:
C57BL/6J-MtgxR1528Btlr/Mmmh
Stock Number:
039568-MU
Citation ID:
RRID:MMRRC_039568-MU
Other Names:
R1528 (G1), C57BL/6J-MtgxR1528Btlr
Major Collection:

Gene Information

Gene Symbol: Ap4e1 [MGI:1336993] (Mus musculus (mouse))
Name: adaptor-related protein complex AP-4, epsilon 1
Synonyms: 2310033A20Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Polr3e [MGI:1349452] (Mus musculus (mouse))
Name: polymerase (RNA) III (DNA directed) polypeptide E
Synonyms: Sin, RPC5
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Setd5 [MGI:1920145] (Mus musculus (mouse))
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Zdhhc17 [MGI:2445110] (Mus musculus (mouse))
Name: zinc finger, DHHC domain containing 17
Synonyms: Hip14, A230053P19Rik, D130071N24Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Erbb4 [MGI:104771] (Mus musculus (mouse))
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: Her4, ErbB4
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Snx1 [MGI:1928395] (Mus musculus (mouse))
Name: sorting nexin 1
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Macf1 [MGI:108559] (Mus musculus (mouse))
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 36,958,377 bp
  • A to T, chromosome 1 at 44,178,241 bp
  • A to C, chromosome 1 at 65,063,057 bp
  • A to T, chromosome 1 at 68,078,582 bp
  • C to T, chromosome 1 at 126,024,922 bp
  • A to G, chromosome 1 at 166,270,495 bp
  • A to C, chromosome 1 at 172,119,582 bp
  • A to T, chromosome 1 at 184,731,547 bp
  • A to G, chromosome 2 at 3,521,041 bp
  • G to A, chromosome 2 at 25,236,098 bp
  • C to A, chromosome 2 at 32,280,704 bp
  • T to C, chromosome 2 at 76,737,068 bp
  • C to T, chromosome 2 at 89,482,553 bp
  • T to A, chromosome 2 at 126,035,818 bp
  • T to A, chromosome 2 at 127,011,823 bp
  • C to T, chromosome 2 at 129,017,069 bp
  • C to A, chromosome 2 at 157,230,055 bp
  • A to G, chromosome 2 at 164,565,908 bp
  • C to T, chromosome 3 at 37,052,535 bp
  • T to C, chromosome 3 at 59,210,289 bp
  • G to A, chromosome 3 at 93,322,794 bp
  • A to T, chromosome 3 at 114,216,995 bp
  • T to G, chromosome 4 at 123,476,014 bp
  • T to G, chromosome 4 at 153,994,508 bp
  • A to G, chromosome 5 at 17,376,542 bp
  • T to C, chromosome 5 at 25,692,294 bp
  • A to G, chromosome 5 at 76,549,564 bp
  • G to A, chromosome 5 at 86,724,210 bp
  • G to A, chromosome 5 at 96,636,819 bp
  • A to T, chromosome 5 at 105,121,792 bp
  • A to T, chromosome 5 at 120,787,989 bp
  • G to A, chromosome 5 at 120,877,037 bp
  • A to T, chromosome 5 at 121,802,108 bp
  • T to C, chromosome 5 at 121,813,530 bp
  • A to T, chromosome 5 at 145,244,601 bp
  • A to T, chromosome 6 at 42,589,665 bp
  • T to A, chromosome 6 at 113,121,738 bp
  • C to A, chromosome 6 at 115,560,028 bp
  • T to C, chromosome 6 at 120,870,794 bp
  • A to C, chromosome 6 at 125,608,291 bp
  • A to T, chromosome 7 at 13,025,636 bp
  • T to C, chromosome 7 at 26,382,298 bp
  • G to A, chromosome 7 at 28,898,266 bp
  • A to G, chromosome 7 at 30,645,661 bp
  • C to G, chromosome 7 at 104,357,299 bp
  • A to T, chromosome 7 at 105,024,764 bp
  • T to A, chromosome 7 at 120,940,597 bp
  • C to A, chromosome 7 at 132,564,269 bp
  • A to G, chromosome 7 at 140,033,783 bp
  • T to G, chromosome 7 at 144,595,566 bp
  • C to A, chromosome 8 at 13,389,693 bp
  • G to A, chromosome 8 at 25,698,767 bp
  • G to T, chromosome 8 at 88,315,450 bp
  • T to C, chromosome 8 at 88,664,589 bp
  • G to T, chromosome 8 at 106,136,752 bp
  • A to T, chromosome 8 at 110,883,241 bp
  • A to T, chromosome 8 at 111,078,866 bp
  • T to A, chromosome 9 at 15,925,091 bp
  • A to G, chromosome 9 at 31,357,286 bp
  • T to C, chromosome 9 at 44,274,723 bp
  • T to C, chromosome 9 at 66,109,543 bp
  • A to T, chromosome 9 at 106,075,220 bp
  • C to A, chromosome 10 at 25,536,078 bp
  • A to G, chromosome 10 at 61,614,237 bp
  • T to C, chromosome 10 at 62,819,045 bp
  • T to C, chromosome 10 at 79,035,564 bp
  • T to A, chromosome 10 at 79,067,696 bp
  • A to T, chromosome 10 at 110,948,189 bp
  • A to G, chromosome 11 at 46,689,471 bp
  • C to A, chromosome 11 at 102,025,512 bp
  • A to G, chromosome 11 at 119,916,715 bp
  • G to T, chromosome 12 at 25,001,760 bp
  • A to T, chromosome 12 at 69,263,198 bp
  • T to A, chromosome 12 at 75,966,100 bp
  • G to A, chromosome 13 at 19,142,028 bp
  • C to T, chromosome 13 at 34,028,147 bp
  • T to A, chromosome 13 at 65,292,165 bp
  • A to T, chromosome 14 at 32,519,022 bp
  • T to C, chromosome 14 at 52,112,224 bp
  • A to T, chromosome 14 at 54,857,705 bp
  • T to A, chromosome 14 at 77,067,939 bp
  • A to T, chromosome 14 at 103,232,597 bp
  • T to C, chromosome 15 at 6,773,805 bp
  • A to T, chromosome 15 at 36,265,655 bp
  • T to C, chromosome 15 at 44,526,724 bp
  • T to C, chromosome 15 at 58,899,215 bp
  • A to G, chromosome 15 at 80,391,774 bp
  • T to A, chromosome 15 at 101,861,088 bp
  • T to A, chromosome 16 at 16,226,299 bp
  • A to G, chromosome 17 at 24,884,414 bp
  • A to G, chromosome 17 at 33,067,241 bp
  • T to C, chromosome 17 at 57,066,935 bp
  • C to A, chromosome 17 at 64,309,222 bp
  • T to G, chromosome 18 at 39,421,397 bp
  • T to A, chromosome 18 at 60,814,999 bp
  • T to A, chromosome 18 at 90,537,086 bp
  • A to G, chromosome 19 at 34,068,670 bp
  • T to G, chromosome 19 at 34,611,672 bp
  • T to C, chromosome 19 at 56,479,995 bp
  • A to T, chromosome X at 21,076,653 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1528 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039568-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.