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Strain Name:
C57BL/6J-MtgxR1535Btlr/Mmmh
Stock Number:
039574-MU
Citation ID:
RRID:MMRRC_039574-MU
Other Names:
R1535 (G1), C57BL/6J-MtgxR1535Btlr
Major Collection:

Strain Information

Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Slc6a1
Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 1
Synonyms: XT-1, Gabt, Xtrp1, Gat1, Gabt1, GAT-1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232333
Homologene: 2290
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 28,777,424 bp
  • C to T, chromosome 1 at 65,168,538 bp
  • T to C, chromosome 1 at 74,159,058 bp
  • T to C, chromosome 1 at 106,982,156 bp
  • T to C, chromosome 1 at 120,171,473 bp
  • T to C, chromosome 1 at 132,525,384 bp
  • T to C, chromosome 1 at 157,230,059 bp
  • A to G, chromosome 1 at 185,289,338 bp
  • A to G, chromosome 2 at 30,087,053 bp
  • A to T, chromosome 2 at 31,420,407 bp
  • T to A, chromosome 2 at 32,204,289 bp
  • T to C, chromosome 2 at 89,283,057 bp
  • C to T, chromosome 2 at 181,134,825 bp
  • T to A, chromosome 3 at 20,266,287 bp
  • C to A, chromosome 3 at 64,126,467 bp
  • C to A, chromosome 3 at 79,481,765 bp
  • G to A, chromosome 3 at 129,303,795 bp
  • T to A, chromosome 3 at 129,690,778 bp
  • A to G, chromosome 3 at 146,002,421 bp
  • G to T, chromosome 4 at 74,013,758 bp
  • G to A, chromosome 4 at 101,156,577 bp
  • C to A, chromosome 4 at 111,459,763 bp
  • A to T, chromosome 4 at 120,761,724 bp
  • A to T, chromosome 4 at 123,440,693 bp
  • C to T, chromosome 4 at 132,497,362 bp
  • C to T, chromosome 4 at 138,097,302 bp
  • A to G, chromosome 4 at 155,896,174 bp
  • T to C, chromosome 5 at 108,802,174 bp
  • G to T, chromosome 5 at 110,708,166 bp
  • A to T, chromosome 5 at 113,263,269 bp
  • A to G, chromosome 5 at 129,908,831 bp
  • T to C, chromosome 6 at 48,975,464 bp
  • A to C, chromosome 6 at 58,939,347 bp
  • T to C, chromosome 6 at 87,981,148 bp
  • G to T, chromosome 6 at 114,307,770 bp
  • T to C, chromosome 6 at 116,024,791 bp
  • T to G, chromosome 6 at 142,664,635 bp
  • G to T, chromosome 7 at 28,757,397 bp
  • C to T, chromosome 7 at 29,529,579 bp
  • T to C, chromosome 7 at 29,698,246 bp
  • T to A, chromosome 7 at 45,794,570 bp
  • T to C, chromosome 7 at 90,452,818 bp
  • A to G, chromosome 7 at 98,593,737 bp
  • A to G, chromosome 7 at 103,051,369 bp
  • C to T, chromosome 7 at 118,529,674 bp
  • T to A, chromosome 7 at 120,540,705 bp
  • T to C, chromosome 7 at 128,252,773 bp
  • A to G, chromosome 8 at 105,014,486 bp
  • C to T, chromosome 9 at 50,764,007 bp
  • A to G, chromosome 9 at 86,587,043 bp
  • A to G, chromosome 10 at 41,485,211 bp
  • A to G, chromosome 10 at 62,108,949 bp
  • C to G, chromosome 10 at 127,665,047 bp
  • C to A, chromosome 11 at 61,673,941 bp
  • T to A, chromosome 11 at 69,226,244 bp
  • A to G, chromosome 12 at 4,870,605 bp
  • A to G, chromosome 12 at 5,084,486 bp
  • T to C, chromosome 12 at 71,951,918 bp
  • C to T, chromosome 12 at 118,018,730 bp
  • T to C, chromosome 13 at 8,886,909 bp
  • A to T, chromosome 13 at 8,940,644 bp
  • T to C, chromosome 13 at 24,439,220 bp
  • A to T, chromosome 13 at 56,523,527 bp
  • A to T, chromosome 13 at 70,791,794 bp
  • A to T, chromosome 14 at 26,816,322 bp
  • G to T, chromosome 14 at 99,079,426 bp
  • C to T, chromosome 14 at 121,546,064 bp
  • T to C, chromosome 15 at 41,869,008 bp
  • T to C, chromosome 15 at 76,702,779 bp
  • A to T, chromosome 15 at 77,777,813 bp
  • T to C, chromosome 15 at 102,093,728 bp
  • T to A, chromosome 16 at 20,189,365 bp
  • A to G, chromosome 16 at 91,659,734 bp
  • A to T, chromosome 17 at 23,175,869 bp
  • A to G, chromosome 17 at 24,439,090 bp
  • G to A, chromosome 17 at 27,434,801 bp
  • T to A, chromosome 18 at 36,005,790 bp
  • A to G, chromosome 18 at 37,896,093 bp
  • C to T, chromosome 18 at 64,545,264 bp
  • G to C, chromosome 18 at 65,350,204 bp
  • A to G, chromosome 19 at 12,120,332 bp
  • T to C, chromosome 19 at 23,979,577 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1535 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039574-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.