Strain Name:
C57BL/6J-MtgxR1539Btlr/Mmmh
Stock Number:
039578-MU
Citation ID:
RRID:MMRRC_039578-MU
Other Names:
R1539 (G1), C57BL/6J-MtgxR1539Btlr
Major Collection:

Gene Information

Gene Symbol: Kif5a [MGI:109564] (Mus musculus (mouse))
Name: kinesin family member 5A
Synonyms: Khc, Kns, D10Bwg0738e, Kif5
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Itga6 [MGI:96605] (Mus musculus (mouse))
Name: integrin alpha 6
Synonyms: 5033401O05Rik, Cd49f
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Ptch1 [MGI:105373] (Mus musculus (mouse))
Name: patched 1
Synonyms: A230106A15Rik, wig, Ptc1, Ptc, Patched 1
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Htr2a [MGI:109521] (Mus musculus (mouse))
Name: 5-hydroxytryptamine (serotonin) receptor 2A
Synonyms: Htr2, Htr-2, 5-HT2A receptor
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Plg [MGI:97620] (Mus musculus (mouse))
Name: plasminogen
Synonyms: Pg
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Frem2 [MGI:2444465] (Mus musculus (mouse))
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, my, 6030440P17Rik, b2b1562Clo, ne
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Rilpl1 [MGI:1922945] (Mus musculus (mouse))
Name: Rab interacting lysosomal protein-like 1
Synonyms: GOSPEL, 2900002H16Rik, 6330559I19Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,498,535 bp
  • A to T, chromosome 1 at 51,799,563 bp
  • A to T, chromosome 1 at 60,083,848 bp
  • C to A, chromosome 1 at 82,711,094 bp
  • G to A, chromosome 1 at 93,137,979 bp
  • A to T, chromosome 1 at 163,962,098 bp
  • C to A, chromosome 2 at 25,273,063 bp
  • A to T, chromosome 2 at 26,472,113 bp
  • A to G, chromosome 2 at 32,378,119 bp
  • A to G, chromosome 2 at 35,088,803 bp
  • G to A, chromosome 2 at 54,857,857 bp
  • T to C, chromosome 2 at 71,838,438 bp
  • A to T, chromosome 2 at 83,880,098 bp
  • G to A, chromosome 2 at 91,938,060 bp
  • A to G, chromosome 2 at 128,782,526 bp
  • G to A, chromosome 2 at 129,118,099 bp
  • A to G, chromosome 2 at 150,975,947 bp
  • T to A, chromosome 2 at 154,223,856 bp
  • A to G, chromosome 2 at 166,926,372 bp
  • T to C, chromosome 3 at 41,604,996 bp
  • G to T, chromosome 3 at 51,247,912 bp
  • T to C, chromosome 3 at 53,654,210 bp
  • A to G, chromosome 3 at 57,646,848 bp
  • T to C, chromosome 3 at 59,097,721 bp
  • A to G, chromosome 3 at 106,486,920 bp
  • A to C, chromosome 3 at 138,223,955 bp
  • T to C, chromosome 4 at 20,054,204 bp
  • T to A, chromosome 4 at 63,379,310 bp
  • A to T, chromosome 4 at 108,021,567 bp
  • A to G, chromosome 5 at 22,060,405 bp
  • T to C, chromosome 5 at 39,614,448 bp
  • T to C, chromosome 5 at 64,926,976 bp
  • A to T, chromosome 5 at 100,883,388 bp
  • T to C, chromosome 5 at 105,274,769 bp
  • A to T, chromosome 5 at 108,191,977 bp
  • T to C, chromosome 5 at 111,100,811 bp
  • T to G, chromosome 5 at 113,952,003 bp
  • T to A, chromosome 5 at 115,574,573 bp
  • T to A, chromosome 5 at 124,515,555 bp
  • T to C, chromosome 5 at 125,109,939 bp
  • T to A, chromosome 5 at 142,094,599 bp
  • A to G, chromosome 5 at 148,335,593 bp
  • A to G, chromosome 6 at 8,591,620 bp
  • A to C, chromosome 6 at 124,191,771 bp
  • T to C, chromosome 7 at 3,110,000 bp
  • A to T, chromosome 7 at 30,972,092 bp
  • G to T, chromosome 7 at 45,725,560 bp
  • G to A, chromosome 7 at 62,378,809 bp
  • A to T, chromosome 7 at 99,871,357 bp
  • G to A, chromosome 7 at 100,890,473 bp
  • A to G, chromosome 7 at 106,891,276 bp
  • A to T, chromosome 7 at 110,049,589 bp
  • T to A, chromosome 7 at 140,419,413 bp
  • T to C, chromosome 8 at 23,093,919 bp
  • C to A, chromosome 8 at 83,670,337 bp
  • T to C, chromosome 9 at 45,930,948 bp
  • T to C, chromosome 9 at 49,037,796 bp
  • A to T, chromosome 9 at 53,809,708 bp
  • A to T, chromosome 9 at 106,952,364 bp
  • G to A, chromosome 9 at 106,996,913 bp
  • T to C, chromosome 9 at 119,127,450 bp
  • T to A, chromosome 9 at 120,424,623 bp
  • C to A, chromosome 10 at 20,479,686 bp
  • T to C, chromosome 10 at 30,771,729 bp
  • T to C, chromosome 10 at 39,532,070 bp
  • T to A, chromosome 10 at 79,491,925 bp
  • T to C, chromosome 10 at 100,496,828 bp
  • A to G, chromosome 10 at 109,767,170 bp
  • G to A, chromosome 10 at 127,237,908 bp
  • T to C, chromosome 10 at 127,584,381 bp
  • G to A, chromosome 10 at 129,570,771 bp
  • T to A, chromosome 11 at 32,533,440 bp
  • T to C, chromosome 11 at 33,297,394 bp
  • T to A, chromosome 11 at 52,120,973 bp
  • C to A, chromosome 11 at 66,037,748 bp
  • A to T, chromosome 11 at 70,169,819 bp
  • T to C, chromosome 11 at 70,253,243 bp
  • A to G, chromosome 11 at 75,771,957 bp
  • A to T, chromosome 11 at 89,441,391 bp
  • A to T, chromosome 11 at 101,771,687 bp
  • G to T, chromosome 11 at 102,155,716 bp
  • A to G, chromosome 11 at 113,652,232 bp
  • G to A, chromosome 12 at 69,741,071 bp
  • A to G, chromosome 12 at 99,912,312 bp
  • T to C, chromosome 12 at 117,931,256 bp
  • T to C, chromosome 13 at 59,715,919 bp
  • T to A, chromosome 13 at 62,371,600 bp
  • A to G, chromosome 13 at 63,541,287 bp
  • C to A, chromosome 13 at 70,605,904 bp
  • A to G, chromosome 13 at 73,944,743 bp
  • T to A, chromosome 13 at 81,503,978 bp
  • T to A, chromosome 14 at 47,245,050 bp
  • C to T, chromosome 14 at 74,645,168 bp
  • A to G, chromosome 14 at 79,062,562 bp
  • T to C, chromosome 14 at 86,656,480 bp
  • A to G, chromosome 15 at 25,987,156 bp
  • A to T, chromosome 15 at 47,820,398 bp
  • C to T, chromosome 16 at 4,876,397 bp
  • G to T, chromosome 16 at 18,584,093 bp
  • T to A, chromosome 16 at 20,642,513 bp
  • A to G, chromosome 16 at 25,884,849 bp
  • G to A, chromosome 16 at 29,958,841 bp
  • A to G, chromosome 16 at 36,495,137 bp
  • A to G, chromosome 16 at 45,757,093 bp
  • C to A, chromosome 16 at 66,784,840 bp
  • T to C, chromosome 16 at 89,158,146 bp
  • T to C, chromosome 17 at 12,411,609 bp
  • T to A, chromosome 17 at 23,717,144 bp
  • A to G, chromosome 18 at 3,433,984 bp
  • A to T, chromosome 18 at 12,043,240 bp
  • T to C, chromosome 18 at 42,149,822 bp
  • T to C, chromosome 18 at 74,219,207 bp
  • C to A, chromosome 19 at 6,246,771 bp
  • C to T, chromosome 19 at 8,965,486 bp
  • T to C, chromosome 19 at 11,896,491 bp
  • A to T, chromosome 19 at 36,733,961 bp
  • G to A, chromosome 19 at 42,767,284 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1539 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039578-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.