Strain Name:
C57BL/6J-MtgxR1584Btlr/Mmmh
Stock Number:
039621-MU
Citation ID:
RRID:MMRRC_039621-MU
Other Names:
R1584 (G1), C57BL/6J-MtgxR1584Btlr
Major Collection:

Gene Information

Gene Symbol: Figla [MGI:1349421] (Mus musculus (mouse))
Name: folliculogenesis specific basic helix-loop-helix
Synonyms: FIG alpha, bHLHc8
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Btrc [MGI:1338871] (Mus musculus (mouse))
Name: beta-transducin repeat containing protein
Synonyms: beta-TrCP, SCF b-TRCP, Slimb, Fbw1a, Beta-Trcp1
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Tln2 [MGI:1917799] (Mus musculus (mouse))
Name: talin 2
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Epha2 [MGI:95278] (Mus musculus (mouse))
Name: Eph receptor A2
Synonyms: Sek-2, Myk2, Eck, Sek2
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Dysf [MGI:1349385] (Mus musculus (mouse))
Name: dysferlin
Synonyms: 2310004N10Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Slc27a4 [MGI:1347347] (Mus musculus (mouse))
Name: solute carrier family 27 (fatty acid transporter), member 4
Synonyms: fatty acid transport protein 4, FATP4
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Lars [MGI:1913808] (Mus musculus (mouse))
Name: leucyl-tRNA synthetase
Synonyms: 2310045K21Rik, 3110009L02Rik
Chromosome: 18
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 39,986,811 bp
  • G to T, chromosome 1 at 82,289,444 bp
  • T to C, chromosome 1 at 156,686,188 bp
  • A to T, chromosome 1 at 170,208,653 bp
  • C to T, chromosome 1 at 181,185,934 bp
  • T to A, chromosome 2 at 5,803,977 bp
  • T to C, chromosome 2 at 24,925,795 bp
  • C to T, chromosome 2 at 25,500,216 bp
  • A to T, chromosome 2 at 26,431,157 bp
  • G to A, chromosome 2 at 26,668,576 bp
  • G to T, chromosome 2 at 28,003,846 bp
  • T to A, chromosome 2 at 29,811,190 bp
  • A to T, chromosome 2 at 48,909,494 bp
  • T to C, chromosome 2 at 69,833,888 bp
  • T to A, chromosome 2 at 85,975,995 bp
  • C to T, chromosome 2 at 89,752,267 bp
  • G to A, chromosome 2 at 119,520,734 bp
  • G to A, chromosome 2 at 122,003,929 bp
  • A to G, chromosome 2 at 128,877,429 bp
  • G to A, chromosome 2 at 180,662,328 bp
  • G to A, chromosome 2 at 181,236,297 bp
  • A to T, chromosome 3 at 72,932,060 bp
  • T to C, chromosome 3 at 89,052,065 bp
  • T to C, chromosome 3 at 108,080,337 bp
  • T to A, chromosome 3 at 129,319,448 bp
  • A to T, chromosome 4 at 33,174,772 bp
  • T to C, chromosome 4 at 106,622,780 bp
  • G to A, chromosome 4 at 116,996,566 bp
  • A to C, chromosome 4 at 118,107,662 bp
  • T to A, chromosome 4 at 126,031,932 bp
  • T to A, chromosome 4 at 141,322,047 bp
  • T to C, chromosome 4 at 141,985,511 bp
  • T to C, chromosome 5 at 34,694,750 bp
  • G to A, chromosome 5 at 45,580,509 bp
  • C to T, chromosome 5 at 72,302,790 bp
  • T to A, chromosome 5 at 104,518,257 bp
  • C to T, chromosome 5 at 112,354,764 bp
  • G to A, chromosome 5 at 150,552,258 bp
  • C to T, chromosome 6 at 8,537,964 bp
  • T to C, chromosome 6 at 17,657,159 bp
  • G to A, chromosome 6 at 29,155,017 bp
  • C to A, chromosome 6 at 48,691,282 bp
  • G to A, chromosome 6 at 72,385,634 bp
  • A to G, chromosome 6 at 83,978,065 bp
  • A to G, chromosome 6 at 84,067,047 bp
  • A to G, chromosome 6 at 86,020,782 bp
  • G to A, chromosome 6 at 87,020,809 bp
  • C to T, chromosome 6 at 147,055,984 bp
  • T to A, chromosome 7 at 3,912,662 bp
  • T to C, chromosome 7 at 75,729,049 bp
  • A to G, chromosome 7 at 103,418,941 bp
  • A to G, chromosome 7 at 120,768,165 bp
  • A to G, chromosome 7 at 139,230,308 bp
  • A to G, chromosome 7 at 140,162,203 bp
  • C to T, chromosome 8 at 61,125,136 bp
  • A to T, chromosome 8 at 71,508,481 bp
  • T to C, chromosome 8 at 110,580,815 bp
  • G to A, chromosome 9 at 38,330,131 bp
  • G to A, chromosome 9 at 65,279,715 bp
  • T to A, chromosome 9 at 67,296,414 bp
  • T to A, chromosome 9 at 67,893,112 bp
  • A to T, chromosome 9 at 86,548,172 bp
  • G to T, chromosome 9 at 88,588,158 bp
  • A to G, chromosome 9 at 119,065,586 bp
  • A to G, chromosome 10 at 127,476,690 bp
  • T to A, chromosome 11 at 4,136,719 bp
  • T to A, chromosome 11 at 5,962,349 bp
  • T to A, chromosome 11 at 23,745,546 bp
  • C to T, chromosome 11 at 60,200,702 bp
  • C to T, chromosome 11 at 72,924,679 bp
  • C to T, chromosome 11 at 77,515,410 bp
  • G to A, chromosome 11 at 94,238,895 bp
  • C to A, chromosome 11 at 101,180,360 bp
  • A to G, chromosome 12 at 24,655,305 bp
  • C to T, chromosome 12 at 40,378,826 bp
  • A to T, chromosome 12 at 50,425,515 bp
  • A to T, chromosome 12 at 70,042,669 bp
  • A to G, chromosome 12 at 78,829,727 bp
  • G to A, chromosome 12 at 84,650,081 bp
  • A to G, chromosome 12 at 98,920,764 bp
  • T to G, chromosome 12 at 110,545,761 bp
  • A to G, chromosome 13 at 14,340,743 bp
  • A to T, chromosome 13 at 23,232,762 bp
  • A to G, chromosome 13 at 29,517,402 bp
  • G to A, chromosome 13 at 47,064,054 bp
  • A to G, chromosome 14 at 34,375,685 bp
  • A to T, chromosome 14 at 50,866,037 bp
  • T to C, chromosome 14 at 65,921,104 bp
  • T to A, chromosome 14 at 124,676,539 bp
  • G to T, chromosome 15 at 4,925,684 bp
  • T to A, chromosome 15 at 65,897,720 bp
  • C to T, chromosome 15 at 76,185,908 bp
  • T to A, chromosome 15 at 82,334,788 bp
  • T to C, chromosome 15 at 92,770,537 bp
  • T to A, chromosome 15 at 100,472,542 bp
  • G to A, chromosome 16 at 18,777,477 bp
  • G to A, chromosome 16 at 20,606,613 bp
  • G to A, chromosome 16 at 32,753,595 bp
  • G to A, chromosome 16 at 35,932,728 bp
  • A to G, chromosome 17 at 18,305,151 bp
  • G to A, chromosome 17 at 28,401,163 bp
  • C to T, chromosome 17 at 33,919,940 bp
  • A to G, chromosome 17 at 34,600,718 bp
  • A to G, chromosome 17 at 46,684,684 bp
  • G to A, chromosome 18 at 42,210,050 bp
  • G to A, chromosome 18 at 84,677,315 bp
  • T to C, chromosome 19 at 13,445,659 bp
  • T to A, chromosome 19 at 45,513,382 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1584 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039621-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.