Strain Name:
C57BL/6J-MtgxR1618Btlr/Mmmh
Stock Number:
039655-MU
Citation ID:
RRID:MMRRC_039655-MU
Other Names:
R1618 (G1), C57BL/6J-MtgxR1618Btlr
Major Collection:

Gene Information

Gene Symbol: Ptprk [MGI:103310] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, receptor type, K
Synonyms: PTPk, RPTPkappa
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Csnk1e [MGI:1351660] (Mus musculus (mouse))
Name: casein kinase 1, epsilon
Synonyms: CKIepsilon, tau, CK1epsilon, CKI epsilon, KC1epsilon
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Camsap3 [MGI:1916947] (Mus musculus (mouse))
Name: calmodulin regulated spectrin-associated protein family, member 3
Synonyms: Nezha, 2310057J16Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Eif4g3 [MGI:1923935] (Mus musculus (mouse))
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: 1500002J22Rik, repro8, 4930523M17Rik, G1-419-52, eIF4GII
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Tnks [MGI:1341087] (Mus musculus (mouse))
Name: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
Synonyms: D130072O21Rik, mTNKS1, TANK1, tankyrase 1, 4930554K12Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Exo1 [MGI:1349427] (Mus musculus (mouse))
Name: exonuclease 1
Synonyms: 5730442G03Rik, Msa
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Sbno1 [MGI:2384298] (Mus musculus (mouse))
Name: strawberry notch 1
Synonyms: sno, 9330180L10Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 39,300,727 bp
  • C to T, chromosome 1 at 56,637,229 bp
  • G to A, chromosome 1 at 87,127,871 bp
  • T to A, chromosome 1 at 163,024,541 bp
  • T to C, chromosome 1 at 171,246,121 bp
  • T to A, chromosome 1 at 175,901,386 bp
  • T to C, chromosome 1 at 188,814,224 bp
  • T to G, chromosome 2 at 86,508,849 bp
  • A to G, chromosome 2 at 88,865,527 bp
  • A to T, chromosome 2 at 90,898,672 bp
  • T to C, chromosome 2 at 121,138,652 bp
  • A to C, chromosome 2 at 131,140,929 bp
  • A to G, chromosome 2 at 156,178,867 bp
  • C to T, chromosome 2 at 163,863,929 bp
  • A to T, chromosome 2 at 181,424,249 bp
  • A to T, chromosome 2 at 181,718,853 bp
  • T to A, chromosome 3 at 51,247,830 bp
  • T to C, chromosome 3 at 63,948,229 bp
  • A to G, chromosome 3 at 79,508,168 bp
  • G to A, chromosome 3 at 92,825,476 bp
  • T to C, chromosome 4 at 19,364,260 bp
  • C to T, chromosome 4 at 33,230,974 bp
  • T to C, chromosome 4 at 57,032,204 bp
  • C to A, chromosome 4 at 138,206,058 bp
  • T to C, chromosome 5 at 22,060,368 bp
  • T to C, chromosome 5 at 107,799,118 bp
  • T to C, chromosome 5 at 124,404,216 bp
  • T to C, chromosome 5 at 137,383,830 bp
  • A to C, chromosome 6 at 120,786,342 bp
  • T to C, chromosome 7 at 23,541,300 bp
  • A to G, chromosome 7 at 36,771,796 bp
  • G to A, chromosome 7 at 64,240,535 bp
  • T to G, chromosome 7 at 79,835,417 bp
  • T to A, chromosome 7 at 80,875,786 bp
  • C to T, chromosome 7 at 85,875,912 bp
  • T to C, chromosome 7 at 126,701,547 bp
  • T to A, chromosome 7 at 139,652,810 bp
  • A to G, chromosome 8 at 3,598,740 bp
  • G to T, chromosome 8 at 34,875,276 bp
  • T to C, chromosome 8 at 54,639,895 bp
  • T to C, chromosome 8 at 71,710,403 bp
  • G to T, chromosome 8 at 84,029,662 bp
  • G to A, chromosome 8 at 84,726,526 bp
  • T to C, chromosome 9 at 18,469,283 bp
  • A to G, chromosome 9 at 22,350,918 bp
  • T to C, chromosome 9 at 69,760,011 bp
  • T to A, chromosome 9 at 92,266,495 bp
  • T to C, chromosome 9 at 123,829,281 bp
  • A to G, chromosome 10 at 19,849,163 bp
  • A to T, chromosome 10 at 28,493,170 bp
  • T to C, chromosome 10 at 81,276,260 bp
  • T to A, chromosome 10 at 85,146,454 bp
  • A to G, chromosome 11 at 11,488,641 bp
  • A to G, chromosome 11 at 101,628,391 bp
  • A to T, chromosome 11 at 109,949,888 bp
  • T to A, chromosome 11 at 114,864,394 bp
  • T to C, chromosome 11 at 114,870,316 bp
  • A to G, chromosome 12 at 7,985,273 bp
  • A to G, chromosome 12 at 29,827,397 bp
  • A to G, chromosome 12 at 64,967,073 bp
  • T to C, chromosome 12 at 118,015,465 bp
  • G to A, chromosome 13 at 98,257,187 bp
  • C to A, chromosome 14 at 3,964,571 bp
  • T to A, chromosome 14 at 3,964,572 bp
  • A to T, chromosome 14 at 46,811,780 bp
  • T to C, chromosome 14 at 74,813,704 bp
  • T to C, chromosome 14 at 105,498,946 bp
  • A to T, chromosome 15 at 79,424,850 bp
  • A to G, chromosome 15 at 89,390,403 bp
  • A to G, chromosome 15 at 92,220,493 bp
  • T to C, chromosome 16 at 4,996,476 bp
  • G to C, chromosome 16 at 34,879,475 bp
  • A to G, chromosome 16 at 56,259,858 bp
  • A to C, chromosome 16 at 62,813,277 bp
  • T to A, chromosome 17 at 23,818,932 bp
  • C to G, chromosome 17 at 25,208,219 bp
  • A to C, chromosome 17 at 27,116,607 bp
  • C to T, chromosome 17 at 34,647,728 bp
  • G to A, chromosome 17 at 38,275,666 bp
  • A to T, chromosome 17 at 46,525,892 bp
  • T to A, chromosome 17 at 46,663,190 bp
  • T to C, chromosome 18 at 22,516,987 bp
  • T to G, chromosome 18 at 67,788,736 bp
  • A to T, chromosome 19 at 18,877,631 bp
  • C to A, chromosome 19 at 39,643,264 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1618 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039655-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.