Strain Name:
C57BL/6J-MtgxR1686Btlr/Mmmh
Stock Number:
039719-MU
Citation ID:
RRID:MMRRC_039719-MU
Other Names:
R1686 (G1), C57BL/6J-MtgxR1686Btlr
Major Collection:

Gene Information

Gene Symbol: Pkd1 [MGI:97603] (Mus musculus (mouse))
Name: polycystin 1, transient receptor poteintial channel interacting
Synonyms: PC1, PC-1, polycystin-1
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Cxcl12 [MGI:103556] (Mus musculus (mouse))
Name: chemokine (C-X-C motif) ligand 12
Synonyms: Sdf1, PBSF/SDF-1, Sdf1a, Sdf1b, TPAR1, TLSF-a, TLSF-b, Scyb12, PBSF, SDF-1
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Satb1 [MGI:105084] (Mus musculus (mouse))
Name: special AT-rich sequence binding protein 1
Synonyms: 2610306G12Rik
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Lyst [MGI:107448] (Mus musculus (mouse))
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Rai14 [MGI:1922896] (Mus musculus (mouse))
Name: retinoic acid induced 14
Synonyms: Ankycorbin, 1700020L11Rik, 1700008J19Rik, Norpeg
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Gprc5a [MGI:1891250] (Mus musculus (mouse))
Name: G protein-coupled receptor, family C, group 5, member A
Synonyms: Raig1, Rai3, Gprc5a
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Gart [MGI:95654] (Mus musculus (mouse))
Name: phosphoribosylglycinamide formyltransferase
Synonyms: Gaps, Prgs
Chromosome: 16
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 24,029,806 bp
  • C to T, chromosome 1 at 88,230,680 bp
  • T to C, chromosome 1 at 88,234,612 bp
  • A to T, chromosome 1 at 132,526,311 bp
  • A to G, chromosome 1 at 135,880,890 bp
  • G to A, chromosome 1 at 173,899,403 bp
  • C to A, chromosome 1 at 179,803,792 bp
  • A to T, chromosome 2 at 20,881,848 bp
  • A to T, chromosome 2 at 27,062,334 bp
  • C to T, chromosome 2 at 80,517,942 bp
  • A to G, chromosome 2 at 118,715,687 bp
  • A to T, chromosome 2 at 120,699,492 bp
  • A to G, chromosome 2 at 121,400,042 bp
  • T to C, chromosome 2 at 130,256,116 bp
  • A to G, chromosome 2 at 146,358,000 bp
  • A to T, chromosome 2 at 180,037,422 bp
  • G to A, chromosome 3 at 63,985,654 bp
  • A to G, chromosome 3 at 75,397,108 bp
  • A to T, chromosome 3 at 75,895,136 bp
  • A to G, chromosome 3 at 85,915,849 bp
  • T to A, chromosome 3 at 88,108,356 bp
  • T to A, chromosome 3 at 96,651,825 bp
  • T to C, chromosome 3 at 134,829,493 bp
  • A to G, chromosome 4 at 34,985,910 bp
  • A to T, chromosome 4 at 43,573,869 bp
  • T to C, chromosome 4 at 96,523,777 bp
  • A to G, chromosome 4 at 118,138,377 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • T to C, chromosome 4 at 135,447,288 bp
  • T to C, chromosome 4 at 155,271,256 bp
  • A to G, chromosome 5 at 8,798,782 bp
  • C to T, chromosome 5 at 25,210,434 bp
  • A to G, chromosome 5 at 43,739,371 bp
  • A to G, chromosome 5 at 97,063,533 bp
  • T to A, chromosome 5 at 104,519,923 bp
  • G to A, chromosome 5 at 136,275,381 bp
  • A to T, chromosome 5 at 142,034,537 bp
  • A to T, chromosome 5 at 149,593,101 bp
  • A to T, chromosome 6 at 48,460,400 bp
  • A to T, chromosome 6 at 83,055,245 bp
  • A to G, chromosome 6 at 117,173,547 bp
  • A to T, chromosome 6 at 135,078,920 bp
  • T to G, chromosome 7 at 12,890,636 bp
  • T to A, chromosome 7 at 15,833,825 bp
  • T to A, chromosome 7 at 17,044,864 bp
  • T to A, chromosome 7 at 62,378,240 bp
  • C to A, chromosome 7 at 79,121,890 bp
  • C to T, chromosome 7 at 120,780,148 bp
  • C to T, chromosome 7 at 141,211,085 bp
  • A to G, chromosome 8 at 86,021,649 bp
  • A to G, chromosome 8 at 95,033,369 bp
  • G to C, chromosome 8 at 106,010,558 bp
  • A to T, chromosome 9 at 7,125,009 bp
  • T to C, chromosome 9 at 18,988,543 bp
  • G to A, chromosome 10 at 4,369,854 bp
  • A to G, chromosome 10 at 61,198,660 bp
  • T to C, chromosome 10 at 80,333,674 bp
  • A to T, chromosome 10 at 100,396,975 bp
  • G to A, chromosome 10 at 100,612,860 bp
  • T to C, chromosome 10 at 127,287,417 bp
  • A to G, chromosome 11 at 54,691,632 bp
  • A to C, chromosome 11 at 59,106,287 bp
  • T to A, chromosome 11 at 67,887,767 bp
  • T to C, chromosome 11 at 100,372,434 bp
  • A to G, chromosome 11 at 100,440,094 bp
  • G to T, chromosome 12 at 59,204,840 bp
  • A to G, chromosome 12 at 72,941,677 bp
  • T to C, chromosome 12 at 78,894,410 bp
  • A to T, chromosome 12 at 86,069,743 bp
  • T to G, chromosome 13 at 3,838,302 bp
  • T to C, chromosome 13 at 4,577,453 bp
  • C to T, chromosome 13 at 11,603,779 bp
  • T to C, chromosome 13 at 13,634,705 bp
  • T to A, chromosome 14 at 47,256,215 bp
  • T to C, chromosome 14 at 50,836,788 bp
  • T to C, chromosome 14 at 56,233,884 bp
  • T to A, chromosome 15 at 10,592,196 bp
  • T to C, chromosome 15 at 89,162,462 bp
  • G to A, chromosome 16 at 17,528,712 bp
  • A to C, chromosome 16 at 28,398,341 bp
  • G to T, chromosome 16 at 91,625,349 bp
  • T to C, chromosome 17 at 24,577,506 bp
  • A to G, chromosome 17 at 35,144,952 bp
  • T to C, chromosome 17 at 51,739,999 bp
  • C to T, chromosome 17 at 57,025,597 bp
  • T to A, chromosome 17 at 69,439,532 bp
  • G to A, chromosome 17 at 71,253,103 bp
  • T to C, chromosome 18 at 67,633,562 bp
  • A to C, chromosome 19 at 42,201,704 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1686 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039719-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.