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Strain Name:
C57BL/6J-MtgxR1698Btlr/Mmmh
Stock Number:
039731-MU
Citation ID:
RRID:MMRRC_039731-MU
Other Names:
R1698 (G1), C57BL/6J-MtgxR1698Btlr
Major Collection:

Strain Information

Mtmr3
Name: myotubularin related protein 3
Synonyms: FYVE-DSP1, 1700092A20Rik, ZFYVE10
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74302
HGNC: HGNC:7451
Homologene: 23662
Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, Nos-1, NO, 2310005C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
Chrna5
Name: cholinergic receptor, nicotinic, alpha polypeptide 5
Synonyms: Acra-5, Acra5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110835
VEGA: 9
HGNC: HGNC:1959
Homologene: 55485
Slc25a13
Name: solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 13
Synonyms: Ctrn, citrin
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 50799
Homologene: 22800
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Snd1
Name: staphylococcal nuclease and tudor domain containing 1
Synonyms: p100 co-activator, Tudor-SN
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56463
Homologene: 8665
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 150,565,369 bp
  • T to C, chromosome 1 at 155,953,358 bp
  • A to T, chromosome 1 at 173,203,371 bp
  • A to G, chromosome 2 at 27,103,127 bp
  • A to G, chromosome 2 at 30,377,997 bp
  • A to T, chromosome 2 at 40,851,806 bp
  • T to C, chromosome 2 at 69,702,469 bp
  • T to C, chromosome 2 at 76,942,915 bp
  • G to T, chromosome 2 at 87,191,737 bp
  • T to A, chromosome 2 at 111,292,560 bp
  • T to C, chromosome 2 at 129,017,358 bp
  • T to A, chromosome 3 at 20,138,051 bp
  • C to A, chromosome 3 at 93,148,458 bp
  • G to A, chromosome 3 at 95,118,695 bp
  • T to C, chromosome 3 at 103,885,798 bp
  • C to T, chromosome 4 at 49,651,498 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • G to T, chromosome 4 at 156,166,558 bp
  • C to T, chromosome 4 at 156,351,038 bp
  • A to G, chromosome 5 at 6,769,509 bp
  • C to A, chromosome 5 at 24,388,188 bp
  • C to T, chromosome 5 at 36,604,530 bp
  • C to T, chromosome 5 at 104,520,510 bp
  • T to C, chromosome 5 at 107,603,550 bp
  • T to C, chromosome 5 at 117,867,232 bp
  • A to C, chromosome 5 at 137,409,669 bp
  • T to C, chromosome 5 at 142,788,703 bp
  • A to G, chromosome 6 at 6,042,655 bp
  • A to G, chromosome 6 at 28,421,111 bp
  • G to T, chromosome 6 at 28,888,253 bp
  • C to T, chromosome 6 at 42,628,017 bp
  • C to T, chromosome 6 at 48,907,905 bp
  • G to A, chromosome 6 at 56,157,008 bp
  • T to C, chromosome 6 at 87,347,169 bp
  • G to A, chromosome 6 at 115,250,403 bp
  • A to C, chromosome 6 at 121,645,158 bp
  • A to T, chromosome 6 at 124,522,766 bp
  • C to T, chromosome 6 at 125,168,590 bp
  • G to A, chromosome 6 at 131,685,584 bp
  • A to G, chromosome 7 at 4,427,791 bp
  • G to A, chromosome 7 at 35,614,521 bp
  • A to C, chromosome 7 at 42,933,614 bp
  • T to C, chromosome 7 at 46,955,624 bp
  • T to A, chromosome 7 at 106,603,008 bp
  • C to T, chromosome 7 at 109,917,380 bp
  • T to C, chromosome 8 at 28,696,478 bp
  • T to A, chromosome 8 at 124,525,683 bp
  • T to A, chromosome 9 at 38,539,256 bp
  • A to G, chromosome 9 at 55,004,642 bp
  • T to C, chromosome 9 at 59,868,181 bp
  • T to C, chromosome 9 at 76,246,453 bp
  • T to C, chromosome 9 at 96,568,375 bp
  • T to C, chromosome 9 at 96,685,462 bp
  • T to A, chromosome 9 at 107,919,980 bp
  • T to A, chromosome 10 at 60,185,703 bp
  • C to T, chromosome 11 at 4,492,825 bp
  • A to G, chromosome 11 at 9,314,507 bp
  • A to T, chromosome 11 at 53,996,096 bp
  • T to C, chromosome 11 at 58,581,251 bp
  • T to A, chromosome 11 at 59,760,258 bp
  • T to C, chromosome 11 at 61,709,602 bp
  • A to G, chromosome 11 at 69,739,877 bp
  • C to T, chromosome 11 at 70,618,673 bp
  • G to A, chromosome 11 at 94,508,143 bp
  • C to A, chromosome 11 at 100,625,163 bp
  • T to A, chromosome 11 at 118,495,221 bp
  • T to C, chromosome 12 at 66,689,335 bp
  • A to T, chromosome 12 at 69,185,719 bp
  • A to G, chromosome 12 at 110,626,992 bp
  • C to T, chromosome 13 at 21,116,565 bp
  • T to G, chromosome 13 at 45,517,044 bp
  • A to T, chromosome 13 at 48,807,630 bp
  • A to T, chromosome 13 at 54,719,581 bp
  • G to A, chromosome 13 at 73,913,352 bp
  • G to A, chromosome 13 at 93,063,519 bp
  • T to A, chromosome 13 at 103,833,731 bp
  • A to G, chromosome 14 at 62,353,632 bp
  • G to A, chromosome 15 at 4,914,140 bp
  • A to T, chromosome 15 at 33,250,126 bp
  • A to G, chromosome 15 at 98,128,318 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • A to G, chromosome 16 at 17,545,646 bp
  • G to A, chromosome 16 at 38,369,491 bp
  • G to A, chromosome 16 at 56,823,570 bp
  • A to T, chromosome 16 at 64,478,059 bp
  • C to T, chromosome 17 at 74,356,160 bp
  • T to C, chromosome 18 at 12,128,929 bp
  • G to A, chromosome 18 at 84,562,157 bp
  • T to C, chromosome 19 at 4,862,207 bp
  • T to C, chromosome 19 at 25,411,317 bp
  • T to G, chromosome 19 at 46,081,431 bp
  • T to C, chromosome 19 at 56,310,016 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1698 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039731-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.